Integrated aerial and destructive phenotyping differentiates chilling stress tolerance during early seedling growth in sorghum

Chiluwal, Anuj; Bheemanahalli, Raju; Perumal, Ramasamy; Asebedo, Antonio R.; Bashir, Elfadil; Lamsal, Abhishes; Šebela, David; Shetty, Nithin J.

doi:10.1016/j.fcr.2018.07.011

Cited by 29 publications

(32 citation statements)

References 32 publications

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“…In the field, early planting imposed significant chilling stress on sorghum plants, resulting in an overall 70-90% decrease in shoot biomass and leaf area compared to regular planting. A similar trend was found for sorghum in the growth chamber temperature treatments in the current and previous studies (Chiluwal et al 2018, Moghimi et al 2019, Ostmeyer et al 2020).…”

Section: Discussionsupporting

confidence: 90%

Evidence for microbiome-dependent chilling tolerance in sorghum

Erlandson

Bheemanahalli

et al. 2021

Preprint

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Early season chilling stress is a major constraint on sorghum production in temperate climates. Chilling-tolerant sorghum is an active area of development, but the potential for early season microbial-enhanced chilling tolerance in sorghum has not yet been explored. In this study, we characterized traits of field-grown sorghum accessions in response to chilling and non-chilling temperatures, then we characterized the effects of chilling temperatures and microbial inoculation on sorghum accession traits in a growth chamber experiment. By comparing sorghum trait responses under chilling stress with and without soil microbial inoculation, we were able to detect a potential microbe-dependent sorghum response to chilling stress. Four sorghum genotypes show a negative response to chilling stress with vs. without microbial inoculation, while five sorghum accessions show increased shoot biomass or/ and leaf area under chilling stress when inoculated with a soil microbiome.

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Section: Discussionsupporting

confidence: 90%

Evidence for microbiome-dependent chilling tolerance in sorghum

Erlandson

Bheemanahalli

et al. 2021

Preprint

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“…In this study, napiergrass showed tremendous ability to regrowth, even after its harvest in October when day/night temperatures had dropped considerably compared with those in the summer. Reduced photosynthesis under low temperature in perennial grasses and other crops have been reported in previous studies (Chiluwal, 2018;Chiluwal et al, 2018aChiluwal et al, , 2018b. Hence, vigorous regrowth observed in this experiment cannot be explained by ongoing photosynthesis, as the temperature regime during the regrowth period was not optimum for plants to photosynthesize at the highest rates, and the day length had shortened due to the fall season.…”

Section: Treatmentsupporting

confidence: 70%

Napiergrass Has Dual Use as Biofuel Feedstock and Animal Fodder

et al. 2019

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Core Ideas Napiergrass can produce higher biomass yield in marginal lands in the southern United States.Napiergrass can be grown as a dual source for feedstocks (bioenergy) and animal fodder.Napiergrass produces regrowth during late fall after its matured biomass harvest.Napiergrass regrowth meets the requirement for good‐quality animal fodder.Napiergrass can provide benefits to farmers following mixed crop and animal farming. Napiergrass (Pennisetum purpureum Schumach) has attracted much attention as an efficient feedstock for biofuel production in sub‐tropical climate such as the southern United States because of its perennial nature and high biomass yield, with low inputs on marginal lands. In this study, we examined if napiergrass can be harvested advantageously both for biofuel feedstock as well as fodder usage. Three harvest treatments, i.e., T1, double harvest per year, first in July (T1H1) and second in November (T1H2); T2, double harvest per year, first in October (T2H1) and second in November (T2H2); and T3, single regular harvest in November (T3), were tested for biomass yield. Plant samples from all five harvests were analyzed for quality parameters to ascertain fodder suitability. Averaged across the study period, highest dry matter annual biomass yield was recorded for T3 (41.6 Mg DM ha−1) followed by T2 (37.2 Mg DM ha−1) and T1 (35.3 Mg DM ha−1). The regrowth after October harvest (T2H2) was found suitable for fodder usage and resulted in a fresh biomass yield of 7.9 Mg ha−1 that contained 213 g kg−1 crude protein (CP), 155 g kg−1 ash, 509 g kg−1 neutral detergent fiber (NDF), and 290 g kg−1 acid detergent fiber (ADF). This study showed that napiergrass can be grown for producing biomass for biofuel production as well as a quality fodder during the late fall season in the southern United States. The results would be of special significance to farmers who follow mixed crop and animal farming.

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“…Ten seeds were placed in each petri dish containing the germination paper moistened with sterile distilled water. The Petri dishes were incubated in an incufridge (RevSci, Minnesota, USA) maintained at 15 °C to impose cold stress, and at 30 °C for control treatment 21 . The germinated seeds were collected five days after germination was initiated and immediately frozen in liquid nitrogen and stored at − 80 °C for RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber

Vennapusa

Somayanda

Doherty

et al. 2020

Sci Rep

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Using existing protocols, RNA extracted from seeds rich in starch often results in poor quality RNA, making it inappropriate for downstream applications. Though some methods are proposed for extracting RNA from plant tissue rich in starch and other polysaccharides, they invariably yield less and poor quality RNA. In order to obtain high yield and quality RNA from seeds and other plant tissues including roots a modified SDS-LiCl method was compared with existing methods, including TRIZOL kit (Invitrogen), Plant RNeasy mini kit (Qiagen), Furtado (2014) method, and CTAB-LiCl method. Modifications in the extraction buffer and solutions used for RNA precipitation resulted in a robust method for extracting RNA in seeds and roots, where extracting quality RNA is challenging. The modified SDS-LiCl method revealed intense RNA bands through gel electrophoresis and a nanodrop spectrophotometer detected ratios of ≥ 2 and 1.8 for A260/A230 and A260/A280, respectively. The absence of starch co-precipitation during RNA extraction resulted in enhanced yield and quality of RNA with RIN values of 7–9, quantified using a bioanalyzer. The high-quality RNA obtained was demonstrated to be suitable for downstream applications, such as cDNA synthesis, gene amplification, and RT-qPCR. The method was also effective in extracting RNA from seeds of other cereals including field-grown sorghum and corn. The modified SDS-LiCl method is a robust and highly reproducible RNA extraction method for plant tissues rich in starch and other secondary metabolites. The modified SDS-LiCl method successfully extracted high yield and quality RNA from mature, developing, and germinated seeds, leaves, and roots exposed to different abiotic stresses.

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Integrated aerial and destructive phenotyping differentiates chilling stress tolerance during early seedling growth in sorghum

Cited by 29 publications

References 32 publications

Evidence for microbiome-dependent chilling tolerance in sorghum

Evidence for microbiome-dependent chilling tolerance in sorghum

Napiergrass Has Dual Use as Biofuel Feedstock and Animal Fodder

A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber

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