Integrated bio-fluorescence sensor

Thrush, E.; Levi, Ofer; Ha, Wonill; Wang, Ke; Smith, Stephen J.; Harris, James S.

doi:10.1016/s0021-9673(03)01361-x

Cited by 60 publications

(38 citation statements)

References 27 publications

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“…Some ways to address this in future designs of the sensor might be to adapt an integrated design with rotational symmetry. 20 Another approach is to incorporate positional sensing into the device itself. This might help avoid any unknown positional changes of the sensor during long-term sensing in one subject.…”

Section: Discussionmentioning

confidence: 99%

“…19 We have developed a miniature semiconductor-based fluorescent sensor containing a vertical-cavity surface-emitting laser (VCSEL), gallium arsenide PIN diode, and a fluorescence emission filter. The sensor is potentially applicable to in vitro diagnostics, 20 in vivo exogenously induced fluorescence, 19 and deep tissue fluorescence via implantation. 21 The sensors have a high sensitivity (5 nM in vitro, and autofluorescence-limited to 50 nM in vivo), 19 and because they are produced with standard semiconductor fabrication techniques, the sensors are scalable for both larger pixel formats and lower-cost manufacturing.…”

Section: Introductionmentioning

confidence: 99%

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Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

Parashurama

O’Sullivan²,

Zerda

et al. 2012

J. Biomed. Opt

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Abstract. Molecular optical imaging is a widespread technique for interrogating molecular events in living subjects. However, current approaches preclude long-term, continuous measurements in awake, mobile subjects, a strategy crucial in several medical conditions. Consequently, we designed a novel, lightweight miniature biosensor for in vivo continuous optical sensing. The biosensor contains an enclosed vertical-cavity surface-emitting semiconductor laser and an adjacent pair of near-infrared optically filtered detectors. We employed two sensors (dual sensing) to simultaneously interrogate normal and diseased tumor sites. Having established the sensors are precise with phantom and in vivo studies, we performed dual, continuous sensing in tumor (human glioblastoma cells) bearing mice using the targeted molecular probe cRGD-Cy5.5, which targets α V β 3 cell surface integrins in both tumor neovasculature and tumor. The sensors capture the dynamic time-activity curve of the targeted molecular probe. The average tumor to background ratio after signal calibration for cRGD-Cy5.5 injection is approximately 2.43 AE 0.95 at 1 h and 3.64 AE 1.38 at 2 h (N ¼ 5 mice), consistent with data obtained with a cooled charge coupled device camera. We conclude that our novel, portable, precise biosensor can be used to evaluate both kinetics and steady state levels of molecular probes in various disease applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

Parashurama

O’Sullivan²,

Zerda

et al. 2012

J. Biomed. Opt

Self Cite

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“…Vertical cavity surface emitting lasers (VCSELs) have been applied by Thrush et al [33] for near-infrared fluorescence detection of fluorophores spun onto a poly- (methyl methacrylate) (PMMA) substrate. The excitation wavelength was 773 nm and the limit of detection for the dye IR-800 was 105 molecules/mm 2 [33].…”

Section: Lasersmentioning

confidence: 99%

“…The excitation wavelength was 773 nm and the limit of detection for the dye IR-800 was 105 molecules/mm 2 [33]. The VCSEL fabrication process was extended, so that the substrate also included a high pass filter and a photodiode for detection, thereby providing self-alignment between all optical components within a pixel area of less than 100 mm [33]. The filter consisted of several dielectric layers that form the top of the VCSEL.…”

Section: Lasersmentioning

confidence: 99%

Recent developments in detection for microfluidic systems

2004

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Microfluidic systems have become more and more important in the field of analytical chemistry. Detection methods on these microsystems are essential for the identification and quantification of chemical species that are being analyzed. This review concentrates on the latest developments of optical detection methods and mass spectrometry in conjunction with microfluidic systems. Electrochemical methods are discussed in another review in the same issue of this journal. Within the optical detection section, topics such as multiplexed detection and the use of waveguides are discussed. Within the discussion of mass spectrometry, the main focus is on electrospray emitters as interfaces between microsystem and spectrometer. Apart from optical detection and mass spectrometry, other techniques such as flame ionization and nuclear magnetic resonance are also mentioned.

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“…For over a decade an assay system based on fluorescence has been considered as one of the most commonly used and well established analytical methods, especially for applications such as high-throughput genomic and proteomic analysis, drug screening tests and real-time PCR (Polymer Chain Reaction) [1][2][3][4][5][6][7][8][9][10]. Recently, simplifying the fluorescence detection system has become more crucial because of the advent of micro total analysis systems (μTAS) many advantages in terms of speed, throughput and costs [11].…”

Section: Introductionmentioning

confidence: 99%

Miniaturized Fluorometer Based on Total Internal Reflector and Condensing Mirror

Jang

Yoo

2013

Journal of the Optical Society of Korea

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A miniaturized fluorescence detection system based on total internal reflection (TIR) configuration, which is applicable to detecting the presence of biological materials labeled with fluorescence dye in micro total analysis systems (μTAS), is proposed. In conventional fluorescence testing and analysis devices, interference between the excitation light beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection system based on TIR configuration that allows the excitation light beam and the emitted light to be spatially perpendicular to each other so as to minimize the interference where fluorescence emission is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being comparable to that in earlier studies.

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Integrated bio-fluorescence sensor

Cited by 60 publications

References 27 publications

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

Recent developments in detection for microfluidic systems

Miniaturized Fluorometer Based on Total Internal Reflector and Condensing Mirror

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