Integrated Functional Omics Analysis of Flavonoid-Related Metabolism in <i>AtMYB12</i> Transcript Factor Overexpressed Tomato

Meng, Xuanlin; Zhao, Xingchen; Ding, Xiangyu; Li, Yang; Cao, Guodong; Zhang, Chu; Su, Xiuli; Liu, Yuanchen; Chen, Xiangfeng; Guo, Jinggong; Cai, Zongwei

doi:10.1021/acs.jafc.0c01894

Cited by 20 publications

(14 citation statements)

References 38 publications

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“…Since phenylpropanoid biosynthesis is dominated by a series of genes, the use of transcription factors activates or suppresses metabolism-related genes, providing effective ways to engineer plants enriched with valuable secondary metabolites [ 2 ]. Although studies revealed that, as an MYB transcription factor, AtMYB12 allows positive regulation of flavonoid biosynthesis, the stability and effectiveness of the AtMYB12 expression in fruit varies widely among proponents [ 30 , 51 ]. In the current study, AtMYB12 was driven by four fruit-specific promoters, downstream regulatory gene expression, and phenylpropanoid accumulation.…”

Section: Introductionmentioning

confidence: 99%

Different Fruit-Specific Promoters Drive AtMYB12 Expression to Improve Phenylpropanoid Accumulation in Tomato

et al. 2022

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Tomato is an economically crucial vegetable/fruit crop globally. Tomato is rich in nutrition and plays an essential role in a healthy human diet. Phenylpropanoid, a critical compound in tomatoes, reduces common degenerative and chronic diseases risk caused by oxidative stress. As an MYB transcription factor, ATMYB12 can increase phenylpropanoid content by activating phenylpropanoid synthesis related genes, such as PAL, C4H, 4CL, CHS. However, the heterologous expression of AtMYB12 in tomatoes can be altered through transgenic technologies, such as unstable expression vectors and promoters with different efficiency. In the current study, the efficiency of other fruit-specific promoters, namely E8S, 2A12, E4, and PG, were compared and screened, and we determined that the expression efficiency of AtMYB12 was driven by the E8S promoter was the highest. As a result, the expression of phenylpropanoid synthesis related genes was regulated by AtMYB12, and the phenylpropanoid accumulation in transgenic tomato fruits increased 16 times. Additionally, the total antioxidant capacity of fruits was measured through Trolox equivalent antioxidant capacity (TEAC) assay, which was increased by 2.4 times in E8S transgenic lines. TEAC was positively correlated with phenylpropanoid content. Since phenylpropanoid plays a crucial role in the human diet, expressing AtMYB12 with stable and effective fruit-specific promoter E8S could improve tomato’s phenylpropanoid and nutrition content and quality. Our results can provide genetic resources for the subsequent improvement of tomato varieties and quality, which is significant for human health.

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Section: Introductionmentioning

confidence: 99%

Different Fruit-Specific Promoters Drive AtMYB12 Expression to Improve Phenylpropanoid Accumulation in Tomato

et al. 2022

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“…In addition, regular analyses, such as growth inhibition, photosynthesis, oxidative stress, and even ultrastructure damage, cannot globally reveal the phenomena and mechanisms of the persistence and recovery of toxicity. Multiomic analysis (e.g., transcriptomics and metabolomics) is an underutilized analysis method that can provide whole profiles of molecular responses; 16,17 thus, this may be a powerful tool for research on the persistence and recovery of environmental toxicity. Diatoms are single-celled plants, mainly photosynthetic eukaryotes, that account for approximately 40% of primary productivity in oceans.…”

Section: ■ Introductionmentioning

confidence: 99%

Persistence and Recovery of ZIF-8 and ZIF-67 Phytotoxicity

Zhang

2021

Environ. Sci. Technol.

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Zeolitic imidazolate frameworks (ZIFs) have been developed quickly and have attracted considerable attention for use in the detection and removal of various pollutants. Understanding the environmental risks of ZIFs is a prerequisite to their safe application by industry and new chemical registration by governments; however, the persistence and recovery of toxicity induced by ZIFs remain largely unclear. This study finds that typical ZIFs (e.g., ZIF-8 and ZIF-67) at a concentration of 0.01−1 mg/L induce significant algal growth inhibition, plasmolysis, membrane permeability, chloroplast damage, and chlorophyll biosynthesis, and the above alterations are recoverable. Unexpectedly, a persistent decrease in reactive oxygen species (ROS) is observed due to the quenching of hydroxyl free radicals. The adverse effects of ZIF-8 are weak and easily alleviated compared with those of ZIF-67. ZIF-8 is internalized mainly by caveolae-mediated endocytosis, while ZIF-67 is internalized mainly by clathrin-mediated endocytosis. Omics studies reveal that the downregulation of mRNA associated with oxidative phosphorylation and the inhibition of chlorophyll and adenosine triphosphate (ATP) synthesis in mitochondria are related to the persistence of phytotoxicity. These findings highlight the phenomena and mechanisms of the persistence and recovery of phytotoxicity, indicating the need to reconsider the environmental risk assessments of ZIFs.

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“…Moreover, it has been reported that the heterologous expression of IbMYB1a positively controls the expression of multiple anthocyanin biosynthetic genes, and results in enhanced-anthocyanin synthesis in tobacco [ 52 ]. Multiple omics analyses have revealed that the main biosynthetic genes PAL , CHS , CHI , F3H , and FLS are upregulated in the flavonoid biosynthesis pathway and resulted in the accumulation of flavonol and flavonoid derivatives in AtMYB12 overexpressed tomato [ 53 ]. Anthocyanin accumulation was enhanced by the overexpression of PAP1/AtMYB75 in transgenic canola ( Brassica napus ) and hop ( Humulus lupulus L.), by regulating the transcription level of flavonoid biosynthesis-related structural genes [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of Three Transcription Factors Differently Regulated Astragalosides Metabolic Biosynthesis in Astragalus membranaceus Hairy Roots

Kim

Park

2022

Plants

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Astragalus membranaceus has been used as a highly popular Chinese herbal medicine for centuries. Triterpenoids, namely astragalosides I, II, III, and IV, represent the main active compounds in this plant species. Transcription factors have a powerful effect on metabolite biosynthesis in plants. We investigated the effect of the Arabidopsis MYB12, production of anthocyanin pigment 1 (PAP1), and maize leaf color (LC) transcription factors in regulating the synthesis of astragaloside metabolites in A. membranaceus. Overexpression of these transcription factors in hairy roots differentially up-regulated these active compounds. Specifically, the overexpression of LC resulted in the accumulation of astragalosides I–IV. The content of astragalosides I and IV were, in particular, more highly accumulated. Overexpression of MYB12 increased the accumulation of astragaloside I in transgenic hairy roots, followed by astragaloside IV, and overexpression of PAP1 resulted in the increased synthesis of astragalosides I and IV. In addition, we found that overexpression of PAP1 together with LC increased astragaloside III levels. At the transcriptional level, several key genes of the mevalonate biosynthetic pathway, especially HMGR1, HMGR2, and HMGR3, were up-regulated differentially in response to these transcription factors, resulting in astragaloside synthesis in the hairy roots of A. membranaceus. Overall, our results indicated that heterologous expression of Arabidopsis MYB12, PAP1, and maize LC differentially affected triterpenoids biosynthesis, leading to the increased biosynthesis of active compounds in A. membranaceus.

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Integrated Functional Omics Analysis of Flavonoid-Related Metabolism in AtMYB12 Transcript Factor Overexpressed Tomato

Cited by 20 publications

References 38 publications

Different Fruit-Specific Promoters Drive AtMYB12 Expression to Improve Phenylpropanoid Accumulation in Tomato

Different Fruit-Specific Promoters Drive AtMYB12 Expression to Improve Phenylpropanoid Accumulation in Tomato

Persistence and Recovery of ZIF-8 and ZIF-67 Phytotoxicity

Heterologous Expression of Three Transcription Factors Differently Regulated Astragalosides Metabolic Biosynthesis in Astragalus membranaceus Hairy Roots

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