Integrin-mediated osteoblastic adhesion on a porous manganese-incorporated TiO2 coating prepared by plasma electrolytic oxidation

Abstract

This study was conducted to evaluate the bioactivity of manganese-incorporated TiO2 (Mn-TiO2) coating prepared on titanium (Ti) plate by plasma electrolytic oxidation (PEO) technique in Ca-, P- and Mn-containing electrolytes. The surface topography, phase and element compositions of the coatings were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectrometry (EDS), respectively. The adhesion of osteoblast-like MG63 cells onto Ti, TiO2 and Mn-TiO2 surfaces was evaluated, and the signal transduction pathway involved was confirmed by the sequential expression of the genes for integrins β1, β3, α1 and α3, focal adhesion kinase (FAK), and the extracellular regulated kinases (ERKs), including ERK1 and ERK2. The results obtained indicated that Mn was successfully incorporated into the porous nanostructured TiO2 coating, and did not alter the surface topography or the phase composition of the coating. The adhesion of the MG63 cells onto the Mn-incorporated TiO2 coating was significantly enhanced compared with that on the Mn-free TiO2 coating and the pure Ti plates. In addition, the enhanced cell adhesion on the Mn-TiO2 coatings may have been mediated by the binding of the integrin subunits, β1 and α1, and the subsequent signal transduction pathway, involving FAK and ERK2. The study indicated that the novel Mn-TiO2 coating has potential for orthopedic implant applications, and that further investigations are required.

A Comparative Study of the Sintering Behavior of Pure and Manganese-Substituted Hydroxyapatite

“…When manganese is substituted into the HA lattice (MnHA) the MnHA has the attractive feature of improving cellular adhesion [20,21,22,23,24]. Manganese (Mn) is an essential trace metal found in all tissues and is required for normal amino acid, lipid, protein, and carbohydrate metabolism [25,26].…”

“…In improving the bioactivity of a material it is critical for the material to support cell attachment and the extracellular matrix which contains bioactive factors to induce regeneration [ 19 ]. When manganese is substituted into the HA lattice (MnHA) the MnHA has the attractive feature of improving cellular adhesion [ 20 , 21 , 22 , 23 , 24 ]. Manganese (Mn) is an essential trace metal found in all tissues and is required for normal amino acid, lipid, protein, and carbohydrate metabolism [ 25 , 26 ].…”

Metal ions: the unfading stars of bone regeneration—from bone metabolism regulation to biomaterial applications

“…177 Earlier, Lüthen et al 178 and Park et al 179 observed no significant positive regulation of osteoblasts by Mn 2+ in vitro. However, a manganese coating constructed by Zhang et al 61 promoted osteoblast adhesion and demonstrated an enhanced osteogenic effect. They proposed that the mechanism involves Mn 2+ binding to integrin subunits β 1 and α 1 and the subsequent mediation of the ERK and focal adhesion kinase (FAK) signaling pathways by Mn 2+ .…”

Hybrid functionalized coatings on Metallic Biomaterials for Tissue Engineering

“…The presence of biological elements in the PEO coating has been reported to improve the bioactivity, resulting in enhanced cell adhesion, proliferation and osteoblastic differentiation [269,270]. In addition, several studies on bioactive element-enriched PEO coatings observed that cell proliferation is rather on par with non-modified Ti and even inferior to that on tissue culture polystyrene (TCPS), because other osteogenesis processes such as cell differentiation (conversion of one type of cells into a more specialized one), including collagen generation (building matrix of the bone), are facilitated instead [257,[271][272][273][274]. In those cases, surface microenvironment features trigger genetic pathways of cell differentiation (with specialized matrix synthesis) instead of the onset of mitosis and subsequent cell proliferation.…”