Intensified nitrogen removal by heterotrophic nitrification aerobic denitrification bacteria in two pilot-scale tidal flow constructed wetlands: Influence of influent C/N ratios and tidal strategies

Tan, Xiaodong; Yang, Yunxia; Li, Xing; Zhou, Zhiwei; Liu, Changjian; Liu, Yongwang; Yin, Wenchao; Fan, Xiao-Yan

doi:10.1016/j.biortech.2020.122803

Cited by 94 publications

(30 citation statements)

References 40 publications

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“…The denitrification process in the CN20 system was mainly performed by aerobic denitrifying bacteria ( Dechloromonas , Rhodobacter , Flavobacterium , and Zoogloea ). Dechloromonas exhibited an excellent aerobic denitrification ability in tidal flow constructed wetland and was the dominant denitrifying and phosphorus-removing bacteria in the single-sludge sequencing batch reactor ( Xu et al, 2018 ; Tan et al, 2020 ). Rhodobacter demonstrated enhancement in the single reactor for nitrogen removal under aerobic conditions ( Chen et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…The amount of Zoogloea , which is correlated with biofloc formation ( Weissbrodt et al, 2013 ) and facilitates aerobic denitrification ( Huang et al, 2015 ; Pishgar et al, 2019 ), was increased in R20. In addition, Dechloromonas , Flavobacterium , and Zoogloea also displayed heterotrophic nitrification ability; therefore, it can conduct heterotrophic nitrification and aerobic denitrification ( Huang et al, 2015 ; Pishgar et al, 2019 ; Tan et al, 2020 ). These results indicated the coexistence of autotrophic nitrification and heterotrophic nitrification in the CN20 system.…”

Section: Resultsmentioning

confidence: 99%

“…Given that high oxygen concentration (>5.0 mg L –1 ) in aquaculture systems significantly inhibits the activity of traditional anaerobic denitrification enzymes, denitrification was assumed to occur only in the inner anoxic region of the bioflocs ( Nootong et al, 2011 ; Hu et al, 2013 ). However, a novel group of aerobic denitrifying bacteria, which can perform denitrification under aerobic condition, has been recently discovered in tidal flow constructed wetlands ( Tan et al, 2020 ), sequencing batch biofilm reactor ( Wang et al, 2017 ; Pan et al, 2020 ), airlift reactor ( Ruan et al, 2016 ), and sequencing batch reactor ( Alzate Marin et al, 2016 ) for wastewater treatment. Many aerobic denitrifiers are also capable of heterotrophic nitrification ( Wang et al, 2017 ; Tan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, a novel group of aerobic denitrifying bacteria, which can perform denitrification under aerobic condition, has been recently discovered in tidal flow constructed wetlands ( Tan et al, 2020 ), sequencing batch biofilm reactor ( Wang et al, 2017 ; Pan et al, 2020 ), airlift reactor ( Ruan et al, 2016 ), and sequencing batch reactor ( Alzate Marin et al, 2016 ) for wastewater treatment. Many aerobic denitrifiers are also capable of heterotrophic nitrification ( Wang et al, 2017 ; Tan et al, 2020 ). However, the complex microbial process of nitrogen removal in biofloc systems was still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

et al. 2020

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In this study, the effect of aerobic denitrification on nitrogen removal was investigated using two zero-discharge biofloc-based recirculating aquaculture systems with representative carbon-to-nitrogen (C/N) ratios of 15 (CN15) and 20 (CN20). Aquaculture wastewater, residual feed, and fish feces were treated in an aerated suspended growth reactor (SGR, dissolved oxygen > 5.0 mg L –1 ). Low toxic NH 3 (<0.1 mg L –1 ) and NO 2 – -N (<0.5 mg L –1 ) concentrations and high NO 3 – -N (83.3%) and NO 2 – -N (100%) removal efficiencies were achieved in the fish tank and SGR of CN20, respectively. The nitrogen mass balances indicated that the gaseous nitrogen loss accounted for 72–75% of the nitrogen input. Illumina sequencing and quantitative polymerase chain reaction revealed that increasing the C/N ratio significantly increased the amount of aerobic denitrifying bacteria ( Dechloromonas , Rhodobacter , Flavobacterium , and Zoogloea ) and aerobic denitrifying functional genes ( napA , nirK , and nosZ ). Autotrophic Nitrosomonas was the dominant nitrifying bacteria in the CN15 system, and autotrophic ( Nitrosomonas ) and heterotrophic nitrifiers coexisted in the CN20 system. Moreover, the functional prediction analysis showed that the carbohydrate, energy, and amino acid metabolisms in the SGR of the latter increased. In conclusion, aerobic denitrification should widely exist in biofloc systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

et al. 2020

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“…The V3–V4 region of 16S rRNA was amplified using universal primers 341 F (5′-CCTACGGGNGGCWGCAG-3′) and 805 R (5′-GACTACHVGGGTATCTAATCC-3′) [ 36 , 37 ]. This primer pair has been chosen because they are the most effective to cover different bacterial groups [ 38 ] and are also commonly used in recent studies on the CW microbiome [ 39 , 40 , 41 , 42 , 43 ]. We added this explanation to the MS (L. XXX).…”

Section: Methodsmentioning

confidence: 99%

Spatial Changes in Microbial Communities along Different Functional Zones of a Free-Water Surface Wetland

et al. 2020

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Constructed wetlands (CWs) are complicated ecosystems that include vegetation, sediments, and the associated microbiome mediating numerous processes in wastewater treatment. CWs have various functional zones where contrasting biochemical processes occur. Since these zones are characterized by different particle-size composition, physicochemical conditions, and vegetation, one can expect the presence of distinct microbiomes across different CW zones. Here, we investigated spatial changes in microbiomes along different functional zones of a free-water surface wetland located in Moscow, Russia. The microbiome structure was analyzed using Illumina MiSeq amplicon sequencing. We also determined particle diameter and surface area of sediments, as well as chemical composition of organic pollutants in different CW zones. Specific organic particle aggregates similar to activated sludge flocs were identified in the sediments. The highest accumulation of hydrocarbons was found in the zones with predominant sedimentation of fine fractions. Phytofilters had the highest rate of organic pollutants decomposition and predominance of Smithella, Ignavibacterium, and Methanothrix. The sedimentation tank had lower microbial diversity, and higher relative abundances of Parcubacteria, Proteiniclasticum, and Macellibacteroides, as well as higher predicted abundances of genes related to methanogenesis and methanotrophy. Thus, spatial changes in microbiomes of constructed wetlands can be associated with different types of wastewater treatment processes.

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Replacing starch with resistant starch (Laminaria japonica) improves water quality, nitrogen and phosphorus budget and microbial community in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)

Wang

Zuo

Wang

et al. 2023

Water Environment Research

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It is essential to increase the use of carbohydrates as an energy source and improve protein synthesis and utilization to reduce ammonia nitrogen emissions. A 60‐day cultural experiment was conducted to assess the impact of resistant starch (kelp meal, Laminaria japonica) replacing starch on water quality, nitrogen and phosphorus budget and microbial community of hybrid snakehead. Approximately 1350 experimental fish (11.4 ± 0.15 g) were randomly divided into control group (C, 20% starch) and four resistant starch groups: low replacement group (LR, 15% starch), medium replacement group (MR, 10% starch), high replacement group (HR, 5% starch) and full replacement group (FR, 0% starch). The crude protein and crude fat content of hybrid snakehead fish fed with the FR diet had the most significant improvement (P < 0.05). However, resistant starch also increased the effectiveness of nitrogen and phosphorus utilization in hybrid snakeheads, which decreased the proportion of total nitrogen and total phosphorus in tail water. The minimum nitrogen and phosphorus emission rate was when the starch level was 6.1%. Denitrifying microbes including Gemmobacter, Rhodobacter, Emticicia and Bosea have become much more prevalent in group FR (P < 0.05). In general, replacing starch with resistant starch can enhance the rate at which nitrogen and phosphorus are used in feeding, lessening water pollution and altering environmental microbial composition. Practitioner Points Resistant starch (RS) improves whole fish nutritional content. Resistant starch improves dietary nitrogen and phosphorus utilization. Resistant starch acts as a carbon source and encourages the colonization of denitrifying bacteria in water.

show abstract

Intensified nitrogen removal by heterotrophic nitrification aerobic denitrification bacteria in two pilot-scale tidal flow constructed wetlands: Influence of influent C/N ratios and tidal strategies

Cited by 94 publications

References 40 publications

Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

Spatial Changes in Microbial Communities along Different Functional Zones of a Free-Water Surface Wetland

Replacing starch with resistant starch (Laminaria japonica) improves water quality, nitrogen and phosphorus budget and microbial community in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)

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