Interaction between benzyladenine, naphthaleneacetic acid and tissue age on adventitious bud formation on leaf sections of Lachenalia hybrids

Niederwieser, J. G.; Staden, J. Van

doi:10.1016/s0254-6299(16)30890-0

Cited by 8 publications

(11 citation statements)

References 9 publications

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“…Lachenalia species appear to vary in shoot rooting response according to the type and concentration of auxin used to stimulate rooting. Niederwieser and van Staden (1992) similarly reported that Lachenalia hybrids vary in their optimal cytokinin requirements for bud stimulation. Therefore, for micropropagation of each Lachenalia genotype, it may be necessary to test several concentrations of various growth regulators for optimal shoot production and rooting.…”

Section: Resultsmentioning

confidence: 97%

“…Lachenalia species can be propagated from seed and bulb cuttings, and some from leaf cuttings (Duncan, 1988) and through tissue culture (Klesser and Nel, 1976;Nel, 1983;Niederwieser and Vcelar, 1990). The effects of explant-tissue age and orientation, sucrose, BA, and NAA on adventitious bud formation from leaf tissue have been investigated (Niederwieser and van Staden, 1992;van Staden and Drewes, 1994;van Rensburg and Vcelar, 1989). Klesser and Nel (1976) and Nel (1983) have published limited information on the in vitro rooting and greenhouse acclimatization of Lachenalia tissue-culture shoots.…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

Ault¹

1995

HortSci

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Shoot formation was obtained from Lachenalia arbuthnotiae W.F. Barker, L. bulbifera (Cyrillo) Engl., and L. purpureo-coerulea Jacq. leaf tissue explants cultured on Murashige and Skoog (MS) medium supplemented with sucrose at 30 g·liter–1, 8.87 μm BA, and 0.44 μm K-NAA. Shoots of all three species rooted on subculture to MS medium supplemented with 0.0, 4.14, or 8.29 μm K-IBA or 0.0, 4.46, or 8.92 μm K-NAA. Maximum percent rooting was ≈81% from treatment with 4.14 μm K-IBA for L. arbuthnotiae and with 8.29 μm K-IBA for L. purpureo-coerulea; it was 59% from treatment with 8.92 μm K-NAA for L. bulbifera. Rooted and nonrooted shoots were acclimatized in a greenhouse. Survival of rooted plants was 93% for L. arbuthnotiae, 95% for L. bulbifera, and 94% for L. purpureo-coerulea. Survival of nonrooted shoots was 71% for L. arbuthnotiae and 91% for L. bulbifera. Chemical names used: 6-benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthaleneacetic acid (K-NAA).

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

Ault¹

1995

HortSci

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“…The general tissue culture medium used at Roodeplaat contains 0.1 mg/l NAA and 2 mg/l BA. Factorial experiments on 6 hybrids showed that NAA was clearly critical for adventitious bud formation, apparently more so than BA (Niederwieser and Van Staden, 1992a). A n interaction exists between the genotype and the optimal concentration of NAA and BA.…”

Section: Origin Of Adventitious Budsmentioning

confidence: 94%

“…Exceptions were observed for some genotypes with a high regeneration potential in vitro (Niederwieser and Van Staden, 1990a) and in vivo (Ndou, 2000). In the study of Niederwieser and Van Staden (1992a) the effect of tissue age was shown to be statistically significant for tissue within the proximal 5 cm of leaves for 5 of the 6 hybrids used. For some of these hybrids, tissue age showed a significant interaction with the BA and/or NAA concentration.…”

Section: Origin Of Adventitious Budsmentioning

confidence: 94%

“…Tissue Age. Tissue age has a pronounced effect on the regeneration potential of leaf tissue in vitro (Niederwieser and Vcelar, 1990;N iederwieser and Van Staden, 1990a;Niederwieser and Van Staden, 1992a) and in vivo (Perrignon, 1992;Ndou, 2000). Young tissue generally has the highest regeneration ability, with a tendency to decrease as the age of the tissue increases (proximal, centre and distal parts of leaves).…”

Section: Origin Of Adventitious Budsmentioning

confidence: 99%

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Review on Adventitious Bud Formation in Lachenalia

Niederwieser¹,

Ndou²

2002

Acta Hortic.

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Lachenalia varieties are propagated vegetatively through adventitious bud formation. In this report, published and unpublished results and observations are reviewed. Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems. In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells. The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme. Tissue age affects both the number of buds formed, as well as the size of bud/bulblet. Young tissue proved to be the best source of tissue in several studies. The physiological stage of the mother plant affected bud formation on bud forming tissue. Once plants enter the post anthesis stage, the regeneration potential decreases markedly. This effect is less evident when plant growth regulators (PGR's) are used in vitro. PGR's stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown. Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases. Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.

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