Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons

Omelchenko, Anton; Menon, Harita; Donofrio, Sarah G; Kumar, Gaurav; Chapman, Heidi M; Roshal, Joshua; Martinez-Montes, Eduardo R.; Wang, Tiffany L.; Spaller, Mark R.; Firestein, Bonnie L.

doi:10.1007/s12035-020-01895-5

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…Research suggests that as the neuron matures, increasing levels of PSD-95 provide an environment that is more friendly to the development of new spines than new branches, and dramatic neuronal morphology changes become more infrequent [ 37 ]. In addition, overexpression of PSD-95 increases spine maturation [ 26 ] which collectively supports a role for PSD-95 during multiple phases of neuronal development, all of which could be affected by ethanol-induced expression changes, depending on the exposure window [ 38 ].…”

Section: Discussionmentioning

confidence: 90%

“…We also know that spines can be differentially regulated by neuronal activity; specifically, dendritic shaft filopodia elongate in response to focal glutamate application [ 48 ], which is particularly relevant for the striatum. Alterations in the number and shape of spines contribute to aberrant synaptic and neural signaling, since the distribution pattern of synapses and the properties of each synapse will determine the connections in and out of each MSN [ 38 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum

Clabough

Ingersoll

Reekes

et al. 2021

IJMS

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Fetal alcohol spectrum disorders are caused by the disruption of normal brain development in utero. The severity and range of symptoms is dictated by both the dosage and timing of ethanol administration, and the resulting developmental processes that are impacted. In order to investigate the effects of an acute, high-dose intoxication event on the development of medium spiny neurons (MSNs) in the striatum, mice were injected with ethanol on P6, and neuronal morphology was assessed after 24 h, or at 1 month or 5 months of age. Data indicate an immediate increase in MSN dendritic length and branching, a rapid decrease in spine number, and increased levels of the synaptic protein PSD-95 as a consequence of this neonatal exposure to ethanol, but these differences do not persist into adulthood. These results demonstrate a rapid neuronal response to ethanol exposure and characterize the dynamic nature of neuronal architecture in the MSNs. Although differences in neuronal branching and spine density induced by ethanol resolve with time, early changes in the caudate/putamen region have a potential impact on the execution of complex motor skills, as well as aspects of long-term learning and addictive behavior.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum

Clabough

Ingersoll

Reekes

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…NMDARs are implicated in the structural regulation of spines, suggesting that the MO-mediated STEP downregulation which helps maintain the surface expression of GluN2B might contribute to the improved dendritic spine density in the MO-treated APP/PS1 mice. Moreover, PSD95 was reported to regulate dendritic arborization and spine number by interacting with cysteine-rich PDZ-binding protein and neuronal cytoskeleton [ 126 ]. Interestingly, the decreased PSD95 in APP/PS1 mice was rescued by MO treatment further implying the role of MO in maintaining dendritic spine density.…”

Section: Discussionmentioning

confidence: 99%

Moringa Oleifera Alleviates Aβ Burden and Improves Synaptic Plasticity and Cognitive Impairments in APP/PS1 Mice

et al. 2022

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Alzheimer’s disease is a global public health problem and the most common form of dementia. Due to the failure of many single therapies targeting the two hallmarks, Aβ and Tau, and the multifactorial etiology of AD, there is now more and more interest in nutraceutical agents with multiple effects such as Moringa oleifera (MO) that have strong anti-oxidative, anti-inflammatory, anticholinesterase, and neuroprotective virtues. In this study, we treated APP/PS1 mice with a methanolic extract of MO for four months and evaluated its effect on AD-related pathology in these mice using a multitude of behavioral, biochemical, and histochemical tests. Our data revealed that MO improved behavioral deficits such as anxiety-like behavior and hyperactivity and cognitive, learning, and memory impairments. MO treatment abrogated the Aβ burden to wild-type control mice levels via decreasing BACE1 and AEP and upregulating IDE, NEP, and LRP1 protein levels. Moreover, MO improved synaptic plasticity by improving the decreased GluN2B phosphorylation, the synapse-related proteins PSD95 and synapsin1 levels, the quantity and quality of dendritic spines, and neurodegeneration in the treated mice. MO is a nutraceutical agent with promising therapeutic potential that can be used in the management of AD and other neurodegenerative diseases.

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“…SZS also regulates the expression of some cell junction related proteins, such as cysteine-rich PDZ-binding protein (Cript), signal-induced proliferation-associated 1-like protein 1 (Sipa1l1), and C-terminal-binding protein 2 (Ctbp2). Cript directly binds to microtubules, linking the microtubule cytoskeleton and PSD-95 family scaffold proteins, and increases the number of dendritic spines 29 . Studies have suggested that the loss of dendritic spines may contribute to memory, learning, and behavioral deficits in patients with neurodegenerative diseases 30 .…”

Section: Discussionmentioning

confidence: 99%

Semen Ziziphi Spinosae attenuates blood–brain barrier dysfunction induced by lipopolysaccharide by targeting the FAK-DOCK180-Rac1-WAVE2-Arp3 signaling pathway

et al. 2022

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Semen Ziziphi Spinosae (SZS) has been extensively used in the daily diet as a functional food for neuroprotective health-benefit in China for many years. However, the neuroprotective mechanism of SZS associated with blood–brain barrier (BBB) integrity remains unexplored. The present study suggests SZS could protect against lipopolysaccharide (LPS)-induced BBB dysfunction. Proteomics indicate that 135 proteins in rat brain are significantly altered by SZS. These differentially expressed proteins are mainly clustered into cell–cell adhesion and adherens junctions, which are closely related with BBB integrity. SZS reversed LPS-induces BBB breakdown by activating the FAK-DOCK180-Rac1-WAVE2-Arp3 pathway. Molecular docking between signaling pathway proteins and identified SZS components in rat plasma reveals that 6”‘-feruloylspinosin, spinosin, and swertisin strongly binds to signaling proteins at multiple amino acid sites. These novel findings suggest a health benefit of SZS in prevention of cerebral diseases and contributes to the further application of SZS as a functional food.

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Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons

Cited by 10 publications

References 46 publications

Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum

Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum

Moringa Oleifera Alleviates Aβ Burden and Improves Synaptic Plasticity and Cognitive Impairments in APP/PS1 Mice

Semen Ziziphi Spinosae attenuates blood–brain barrier dysfunction induced by lipopolysaccharide by targeting the FAK-DOCK180-Rac1-WAVE2-Arp3 signaling pathway

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