Interaction mechanism between TiO<sub>2</sub> nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

Tereshchenko, Alla; Smyntyna, V.; Ramanavičius, Arūnas

doi:10.1039/c8ra07347c

Cited by 24 publications

(32 citation statements)

References 37 publications

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“…The incubation of gp51&BSA/ZnO-NR/glass and gp51&BSA/PAH/ ZnO-NR/glass based structures in 'BLV-positive' aliquots resulted in the decrease of PL due to interaction of immobilized gp51 proteins with anti-BLV antibodies. Form shifts of PL-spectra maximums we can conclude that the dominating effect on photoluminescent centres of ZnO-NRs based on electrostatic interaction between ZnO-NRs and immobilized gp51 in similar way as it was determined in the case of interaction between TiO 2 and gp51 [36].…”

Section: Testing Of Optical Immunosensor Based On Zno-nrssupporting

confidence: 66%

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Viter

Savchuk

Стародуб

et al. 2019

Sensors and Actuators B: Chemical

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Bovine leukaemia virus (BLV) proteins gp51, which are serving as antigens for specific antibodies against BLV proteins (anti-gp51), were applied as biological recognition part in the design of immunosensor devoted for the determination of anti-gp51. The efficiency of the immobilization of BLV proteins gp51 on ZnO nanorod (ZnO-NR) modified glass (ZnO-NR/glass) surface was evaluated. The formation of antigen-antibody complex on the ZnO/glass modified by the BLV proteins gp51 (gp51/ZnO-NR/glass) was investigated by the determination of changes in ZnO photoluminescence. The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was evaluated. Bovine serum albumin (BSA) was applied for the modification of sensing gp51 layer in order to form gp51&BSA layer with advanced selectivity. Polyallylamine hydrochloride (PAH) was applied in order to improve the immobilization of gp51 and BSA based sensing layer (gp51&BSA) on the surface of ZnO-NR/glass. PAH was applied during the formation of gp51&BSA/PAH/ZnO-NR/glass structure. Some aspects of the mechanism of interaction between biomolecules (gp51, BSA and anti-gp51) and ZnO-NR during the preparation and action of gp51&BSA/ZnO-NR/glass-and gp51&BSA/PAH/ZnO-NR/glass-based immunosensors have been discussed.

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Section: Testing Of Optical Immunosensor Based On Zno-nrssupporting

confidence: 66%

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Viter

Savchuk

Стародуб

et al. 2019

Sensors and Actuators B: Chemical

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“…The discovery of the latter effect facilitated the exploitation of catalytic TiO 2 properties in the design of sensors for gases and gaseous materials. Due to remarkable properties, many different TiO 2 -based structures have found applications in various technological areas including biosensors [4,5] and chemical sensors [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

TiO2-x/TiO2-Structure Based ‘Self-Heated’ Sensor for the Determination of Some Reducing Gases

Ramanavičius

Tereshchenko

Karpicz

et al. 2019

Sensors

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In this research we report the gas-sensing properties of TiO2-x/TiO2-based hetero-structure, which was ‘self-heated’ by current that at constant potential passed through the structure. Amperometric measurements were applied for the evaluation of sensor response towards ethanol, methanol, n-propanol and acetone gases/vapours. The sensitivity towards these gases was based on electrical resistance changes, which were determined by amperometric measurements of current at fixed voltage applied between Pt-based contacts/electrodes deposited on the TiO2-x/TiO2-based layer. X-ray diffraction (XRD) analysis revealed the formation of TiO2-x/TiO2-based hetero-structure, which is mainly based on Ti3O5/TiO2 formed during the hydro-thermal oxidation-based sensing-layer preparation process. Additionally, photoluminescence and time-resolved photoluminescence decay kinetics-based signals of this sensing structure revealed the presence of TiO2 mainly in the anatase phase in the TiO2-x/TiO2-based hetero-structure, which was formed at 400 °C annealing temperature. The evaluation of TiO2-x/TiO2-based gas-sensing layer was performed at several different temperatures (25 °C, 72 °C, 150 °C, 180 °C) and at these temperatures different sensitivity to the aforementioned gaseous materials was determined.

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“…Various metal oxides (TiO 2 , ZnO, SnO 2 , WO 3 , etc.) are widely used for numerous technological purposes including application in analytical signal transduction systems [ 2 , 3 ]. Nanostructured metal oxides are especially attractive in sensors due to their electrochemical properties and their large chemically active surface.…”

Section: Introductionmentioning

confidence: 99%

Tuning the Photo-Luminescence Properties of WO3 Layers by the Adjustment of Layer Formation Conditions

et al. 2020

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In this research we have applied sol-gel synthesis for the deposition of tungsten (VI) oxide (WO3) layers using two different reductants (ethanol and propanol) and applying different dipping times. WO3 samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR), photoluminescence (PL) and time-resolved photoluminescence decay methods. Photoelectrochemical (PEC) behaviour of synthesized coatings was investigated using cyclic voltammetry in the dark and under illumination. Formation of different structures in differently prepared samples was revealed and significant differences in the PL spectra and PEC performance of the samples were observed. The results showed that reductant used in the synthesis and dipping time strongly influenced photo-electrochemical properties of the coatings. Correlation between the morphology, PL and PEC behaviour has been explained.

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Interaction mechanism between TiO₂ nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

Abstract: In this research a mechanism of interaction between a semiconducting TiO2 layer and bovine leukemia virus protein gp51, applied in the design of photoluminescence-based immunosensors, is proposed and discussed.

Cited by 24 publications

References 37 publications

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

TiO2-x/TiO2-Structure Based ‘Self-Heated’ Sensor for the Determination of Some Reducing Gases

Tuning the Photo-Luminescence Properties of WO3 Layers by the Adjustment of Layer Formation Conditions

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Interaction mechanism between TiO2 nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

Abstract: In this research a mechanism of interaction between a semiconducting TiO2 layer and bovine leukemia virus protein gp51, applied in the design of photoluminescence-based immunosensors, is proposed and discussed.

Cited by 24 publications

References 37 publications

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

TiO2-x/TiO2-Structure Based ‘Self-Heated’ Sensor for the Determination of Some Reducing Gases

Tuning the Photo-Luminescence Properties of WO3 Layers by the Adjustment of Layer Formation Conditions

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Interaction mechanism between TiO₂ nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors