Interaction of 2,4-dichlorophenoxyacetic acid (2,4-D) with cell and model membranes

Suwalsky, Mario; Benites, Maritza; Villena, Fernando Pardo-Manuel de; Aguilar, Felipe; Sotomayor, Carlos P.

doi:10.1016/s0005-2736(96)00173-3

Cited by 52 publications

(25 citation statements)

References 23 publications

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“…2). Previous investigations performed by Suwalsky et al (1996) as well as by Bukowska and Zatorska (2003) proved that 2,4-D-Na induced changes in erythrocytes morphology. Suwalsky et al (1996) used scanning electron microscopy and revealed that normal red blood cells were converted into echinocytes, probably due to a massive insertion of the 2,4-D-Na into the outer lipid monolayer of the plasma membrane.…”

Section: Discussionmentioning

confidence: 85%

“…The analysis of anisotropy value for ANS showed that the phenoxyherbicides examined at their highest concentration (2 mM) caused an increase in erythrocytes membrane fluidity (Table 1), which was surely connected with their impact on protein-lipid interactions. Suwalsky et al (1996) studied the effect of 2,4-D on dimyristophosphatidylcholine large unilamellar vesicles (DMPC LUV). They observed that 2,4-D was incorporated into DMPC LUV bilayer, deeply penetrated hydrocarbonchains region, and provoked its structural disorder.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, discussed compounds induced lipid and protein peroxidation in red blood cells (Duchnowicz et al 2005;Bukowska et al 2008). The studies conducted by Suwalsky et al (1996) and Bukowska (2004) also illustrated that phenoxyherbicides induced morphological changes in human erythrocytes, which resulted in a formation of echinocytes.…”

Section: Introductionmentioning

confidence: 99%

“…To explain the mechanism of phenoxyherbicides action, we used high concentrations of these substances (ranging from 0.5 to 2 mM), which may penetrate human cells only as a result of acute poisoning. The chosen doses corresponded to the amounts of 2,4-D, 2,4-5-T and MCPA, which induced changes of various biochemical parameters studied in investigations of other authors (Suwalsky et al 1996;Kaioumova et al 2001;Duchnowicz et al 2002Duchnowicz et al , 2005Tuschl & Schwab 2003;Maire et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Comparison of the effect of phenoxyherbicides on human erythrocyte membrane (in vitro)

et al. 2011

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The molecular mechanisms of phenoxyherbicides action in animals have been insufficiently studied. Now, we have investigated the interaction of sodium salts of phenoxyherbicides, e.g., 2,4-dichlorophenoxyacetic acid (2,4-D-Na), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T-Na) and 4-chloro-2-methylphenoxyacetic acid (MCPA-Na) with human erythrocytes. In this study, we evaluated the effect of these compounds on erythrocyte membrane fluidity as well as changes in membrane proteins content. It was observed that all of the compounds studied altered membrane fluidity, changed the size and shape of the erythrocytes and provoked echinocytes formation. It was also revealed that 2,4-D-Na and 2,4,5-T-Na changed the content of erythrocyte membrane proteins mainly by a decrease in the level of spectrin and low molecular weight proteins. The comparison of the action of phenoxyherbicides examined showed that 2,4,5-T-Na caused the greatest changes in the erythrocytes membrane, whereas MCPA-Na induced the lowest alterations in the incubated cells. It must be noted that changes of the investigated parameters were observed only at presence of significant concentrations of these compounds that may penetrate human organism only as a result of acute poisoning.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of the effect of phenoxyherbicides on human erythrocyte membrane (in vitro)

et al. 2011

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“…Toxic influence of 2,4-D may provoke disturbances in bilayer phospholipid structure that plays an important role in the correct function of cell membrane. Phenoxyherbicides interact with proteins and lipids of erythrocyte membrane (Suwalsky and Berites, 1996). Indeed, Janik and Wolf (1992) have demonstrated the inhibitory effect of chlorinated compounds on the Ca-ATPase which indicates a toxic effect to human erythrocytes functions.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Oxidative Stress Induced by the 2,4-Dichlorophenoxyacetic Herbicide

Tayeb¹,

Nakbi²,

Chaieb³

et al. 2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid

Teixeira¹,

Santos²,

Fernandes³

et al. 2005

Proteomics

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The intensive use of herbicides may give rise to a number of toxicological problems in non-target organisms and has led to the emergence of resistant weeds. To gain insights into the mechanisms of adaptation to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), we have identified variations in protein expression level in the eukaryotic experimental model Saccharomyces cerevisiae exposed to herbicide aggression, based on two-dimensional gel electrophoresis. We show results suggesting that during the adaptation period preceding the resumption of inhibited exponential growth under herbicide stress, the antioxidant enzyme Ahp1p and the heat shock proteins Hsp12p and Ssb2p (or Ssb1p) are present in higher amounts. The increased level of other enzymes involved in protein (Cdc48p) and mRNA (Dcp1p) degradation, in carbohydrate metabolism (Eno1p, Eno2p and Glk1p) and in vacuolar H(+)-ATPase (V-ATPase) function (Vma1p and Vma2p, two subunits of the peripheral catalytic sector) was also registered. V-ATPase is involved in the homeostasis of intracellular pH and in the compartmentalization of amino acids and other metabolites in the vacuole. The increased expression of amino acid biosynthetic enzymes (Arg1p, Aro3p, Aro8p, Gdh1p, His4p, Ilv3p and Met6p), also suggested by comparative analysis of the proteome, was correlated with the reduction of amino acid concentration registered in both the vacuole and the cytosol of 2,4-D-stressed cells, possibly due to the disturbance of vacuolar and plasma membrane functions by the lipophilic acid herbicide.

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Interaction of 2,4-dichlorophenoxyacetic acid (2,4-D) with cell and model membranes

Cited by 52 publications

References 23 publications

Comparison of the effect of phenoxyherbicides on human erythrocyte membrane (in vitro)

Comparison of the effect of phenoxyherbicides on human erythrocyte membrane (in vitro)

Oxidative Stress Induced by the 2,4-Dichlorophenoxyacetic Herbicide

A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid

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