Interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and human matrix metalloproteinase 7 (MMP-7) as examined by MMP-7 activity and ANS fluorescence

Samukange, Vimbai; Yasukawa, Kiyoshi; Inouye, Kuniyo

doi:10.1093/jb/mvs025

Cited by 11 publications

(5 citation statements)

References 42 publications

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“…MMP-7/matrilysin is an enzyme that degrades collagens I, III, IV and V, fibronectin, vitronectin, laminin and elastin [47] and is expressed in variety of cancers including colon, prostate, esophageal and HNSCCs [48,49,50,51,52]. Gene expression analyses also show that MMP-7 is significantly overexpressed in almost all HNSCC datasets (Figure 2 and Table 1, Table 2, Table 3, Table 4 and Table 5).…”

Section: Roles Of Mmps In Hnsccsmentioning

confidence: 99%

Matrix Metalloproteinases: The Gene Expression Signatures of Head and Neck Cancer Progression

Iizuka

Ishimaru

Kudo

2014

Cancers

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Extracellular matrix degradation by matrix metalloproteinases (MMPs) plays a pivotal role in cancer progression by promoting motility, invasion and angiogenesis. Studies have shown that MMP expression is increased in head and neck squamous cell carcinomas (HNSCCs), one of the most common cancers in the world, and contributes to poor outcome. In this review, we examine the expression pattern of MMPs in HNSCC by microarray datasets and summarize the current knowledge of MMPs, specifically MMP-1, -3, -7 -10, -12, -13, 14 and -19, that are highly expressed in HNSCCs and involved cancer invasion and angiogenesis.

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Section: Roles Of Mmps In Hnsccsmentioning

confidence: 99%

Matrix Metalloproteinases: The Gene Expression Signatures of Head and Neck Cancer Progression

Iizuka

Ishimaru

Kudo

2014

Cancers

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“…MMP7 is a Wnt-targeting gene that has been detected in several cancers, such as prostate, colon, stomach, lung, and breast [13-17] and degrades components of the extracellular matrix (ECM), including collagens (I, III, IV, and V), fibronectin, vitronectin, laminin, and elastin [18]. In the oral region, Chuang et al demonstrated that MMP7 is closely related to invasion in OSCCs of buccal mucosa [19].…”

Section: Introductionmentioning

confidence: 99%

Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation

et al. 2013

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BackgroundGlutamate decarboxylase 1 (GAD1), a rate-limiting enzyme in the production of γ-aminobutyric acid (GABA), is found in the GABAergic neurons of the central nervous system. Little is known about the relevance of GAD1 to oral squamous cell carcinoma (OSCC). We investigated the expression status of GAD1 and its functional mechanisms in OSCCs.MethodsWe evaluated GAD1 mRNA and protein expressions in OSCC-derived cells using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunoblotting analyses. To assess the critical functions of GAD1, i.e., cellular proliferation, invasiveness, and migration, OSCC-derived cells were treated with the shRNA and specific GAD1 inhibitor, 3-mercaptopropionic acid (3-MPA). GAD1 expression in 80 patients with primary OSCCs was analyzed and compared to the clinicopathological behaviors of OSCC.ResultsqRT-PCR and immunoblotting analyses detected frequent up-regulation of GAD1 in OSCC-derived cells compared to human normal oral keratinocytes. Suppression of nuclear localization of β-catenin and MMP7 secretion was observed in GAD1 knockdown and 3-MPA-treated cells. We also found low cellular invasiveness and migratory abilities in GAD1 knockdown and 3-MPA-treated cells. In the clinical samples, GAD1 expression in the primary OSCCs was significantly (P < 0.05) higher than in normal counterparts and was correlated significantly (P < 0.05) with regional lymph node metastasis.ConclusionsOur data showed that up-regulation of GAD1 was a characteristic event in OSCCs and that GAD1 was correlated with cellular invasiveness and migration by regulating β-catenin translocation and MMP7 activation. GAD1 might play an important role in controlling tumoral invasiveness and metastasis in oral cancer.

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“…MMP-7 is the smallest of the matrix metalloproteinases (MMPs) in size, with 28 kDa latent pro-form, and the mature form being 19 kDa [ 14 ]. MMP-7 is composed of a five-stranded β-sheet and three α-helices, with zinc-containing active domain, and other zinc and calcium ions necessary for structural stability [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Elevated matrix metalloproteinase-7 expression promotes metastasis in human lung carcinoma

Han

et al. 2015

World J Surg Onc

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BackgroundMatrix metalloproteinase 7 (MMP-7) promotes tumor invasion and metastasis in several cancers. However, its role in lung cancer progression is understudied. In this study, we investigated the correlation between MMP-7 expression and lung cancer pathology.MethodsWe searched the databases PubMed, Embase, Web of Science, Cochrane Library, CISCOM, CINAHL, China BioMedicine (CBM) and China National Knowledge Infrastructure (CNKI) for scientific literature relevant to MMP-7 and lung cancer. Carefully selected studies were pooled and ORs with 95% CI were calculated. Subgroup analyses and publication bias were analyzed to understand the retrieved data in greater detail. Version 12.0 STATA software was used for statistical analysis.ResultsWe retrieved a total of 121 studies through database searches. Finally, 14 cohort studies satisfied our inclusion/exclusion criteria, and these 14 studies, published between 2004 and 2012, were selected for meta-analysis to understand the influence of MMP-7 expression in lung cancer progression. Our results showed consistent differences in MMP-7 expression when comparisons were made between TNM I-II versus III-IV (OR = 1.82, 95% CI: 1.19 to 2.78, P = 0.006); histologic grade 1 to 2 versus 3 to 4 (OR = 1.67, 95% CI: 1.14 to 2.42, P = 0.008); and lymph node-negative versus lymph node-positive samples (OR = 2.81, 95% CI: 1.73 to 4.58, P <0.001), with significantly higher MMP-7 expression levels found in the more advanced stages. Subgroup analysis showed that age was not the factor influencing the associations between histologic grade, LN metastasis and MMP-7 expression in lung cancer patients, as both under 60 and over 60 age groups showed strong correlations (all P <0.05). However, when TNM staging was analyzed for its association with MMP-7 expression, only patients under age 60 showed a statistically significant correlation.ConclusionsOur meta-analysis results revealed that MMP-7 overexpression is associated with advanced TNM and histological grades, and is linked to aggressive LN metastasis in lung cancer patients; thus MMP-7 is a useful biomarker to assess the disease status in lung cancers.

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Interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and human matrix metalloproteinase 7 (MMP-7) as examined by MMP-7 activity and ANS fluorescence

Cited by 11 publications

References 42 publications

Matrix Metalloproteinases: The Gene Expression Signatures of Head and Neck Cancer Progression

Matrix Metalloproteinases: The Gene Expression Signatures of Head and Neck Cancer Progression

Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation

Elevated matrix metalloproteinase-7 expression promotes metastasis in human lung carcinoma

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