Interaction of antifungal peptide BP15 with Stemphylium vesicarium , the causal agent of brown spot of pear

Puig, Mireia; Moragrega, C.; Ruz, L.; Calderón, Claudia E.; Cazorla, Francisco M.; Seguí, Emilio Montesinos; Llorente, Isidre

doi:10.1016/j.funbio.2015.10.007

Cited by 26 publications

(21 citation statements)

References 38 publications

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“…In this study, the effect of GA treatment on the cell wall integrity of G. citri-aurantii was also clarified by the CFW staining method. CFW is a fluorescent stain that binds strongly to structures containing cellulose and chitin in the cell wall of fungi (Puig et al 2016). As revealed in Fig.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Yang

Ouyang

et al. 2019

J Appl Microbiol

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Aims This study investigated the inhibitory effect of glutaraldehyde (GA) on sour rot in citrus fruit and the underlying antifungal mechanism on mycelial growth of the causative pathogen Geotrichum citri‐aurantii. Methods and Results Glutaraldehyde exhibited a strong inhibitory effect on G. citri‐aurantii, with a minimum inhibitory and fungicidal concentration (MFC) of 1·00 µl ml−1. In addition, in vivo application of GA (1 × MFC and 5 × MFC) reduced the disease incidence of sour rot in citrus fruit by 60 and 80% respectively. Scanning electron microscopy results revealed that the morphology of G. citri‐aurantii mycelia was greatly altered by GA treatment. Propidium iodide and Calcofluor White Staining revealed that the membrane permeability, rather than the cell wall integrity, of G. citri‐aurantii mycelia was severely disrupted after the addition of GA. Massive accumulation of malonaldehyde and reactive oxygen species as well as an increase in lipoxygenase activity were observed. Conclusion These results indicate that GA may inhibit the mycelia growth of G. citri‐aurantii through a membrane damage mechanism induced by membrane peroxidation. Significance and Impact of the Study Glutaraldehyde is expected to be a novel fungicide for controlling sour rot in citrus fruit.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Yang

Ouyang

et al. 2019

J Appl Microbiol

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“…Fruit lesions gradually expand and deepen in round-shaped brown areas that can decay [3]. Infected fruits lose their commercial value and severe outbreaks can lead to fruit abscission prior to harvest [4].…”

Section: Introductionmentioning

confidence: 99%

Potential Application of Propolis Extracts to Control the Growth of Stemphylium vesicarium in “Rocha” Pear

et al. 2020

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Stemphylium vesicarium (Wallr.) E. G. Simmons is the pathogen responsible of brown spot disease in pear and has become one of the main concerns for European pear producers. In Portugal, S. vesicarium is responsible for significant yield reduction and economic losses in “Rocha” pear (Pyrus communis L. cv Rocha) production. Considering the antimicrobial potential of propolis, the high incidence of brown spot in pears and the emergence of fungicides resistance in S. vesicarium, this study aimed to evaluate the potential of Portuguese propolis as an alternative strategy to control brown spot disease in “Rocha” pear. In vitro assays showed that propolis extracts were able to inhibit up to 90% the S. vesicarium mycelial growth. In vivo assays in artificially wounded and inoculated “Rocha” pears showed that, compared to the control, the disease incidence decreased up to 25% and the lesions diameter up to 57%, in fruits treated with propolis. Moreover, propolis seems to be more efficient in reducing the disease incidence when applied after pathogen inoculation (curative assay) than when applied before pathogen inoculation (prophylactic assay). Thus, the results suggest that propolis extracts have potential to be applied as part of an integrated approach for the control of brown spot of pear.

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“…In particular, certain peptides from the CECMEL11 library (LIPPSO-CIDSAV, University of Girona, Girona, Spain), which is composed of de novo designed and synthetically produced cecropin A-melittin hybrid linear undecapeptides, have been derived from the peptide Pep3 (WKLFKKILKVL-NH 2 ) and evaluated for Stemphylium vesicarium infection control in pears ( Badosa et al, 2009 ). For example, BP15 (KKLFKKILKVL-NH 2 ) inhibited S. vesicarium growth, produced morphological alterations to germ tubes and induced cell membrane disruption ( Ferre et al, 2006 ; Puig et al, 2014 , 2016 ). BP15 also could control the infection caused by P. digitatum on citrus fruit ( Muñoz et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Control of Citrus Post-harvest Green Molds, Blue Molds, and Sour Rot by the Cecropin A-Melittin Hybrid Peptide BP21

et al. 2018

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In this study, the activity of the cecropin A-melittin hybrid peptide BP21 (Ac-FKLFKKILKVL-NH2) in controlling of citrus post-harvest green and blue molds and sour rot and its involved mechanism was studied. The minimum inhibitory concentrations of BP21 against Penicillium digitatum, Penicillium italicum, and Geotrichum candidum were 8, 8, and 4 μmol L-1, respectively. BP21 could inhibit the growth of mycelia, the scanning electron microscopy results clearly showed that the mycelia treated with BP21 shrank, formed a rough surface, became distorted and collapsed. Fluorescent staining with SYTOX Green (SG) indicated that BP21 could disintegrate membranes. Membrane permeability parameters, including extracellular conductivity, the leakage of potassium ions, and the release of cellular constituents, visibly increased as the BP21 concentration increased. Gross and irreversible damage to the cytoplasm and membranes was observed. There was a positive correlation between hemolytic activity and the concentration of BP21. These results suggest peptide BP21 could be used to control citrus post-harvest diseases.

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Interaction of antifungal peptide BP15 with Stemphylium vesicarium , the causal agent of brown spot of pear

Cited by 26 publications

References 38 publications

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Potential Application of Propolis Extracts to Control the Growth of Stemphylium vesicarium in “Rocha” Pear

Control of Citrus Post-harvest Green Molds, Blue Molds, and Sour Rot by the Cecropin A-Melittin Hybrid Peptide BP21

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