“…In order to quantitatively examine the interaction of THPP with the protein, the binding constants were determined from the fluorescence intensity changes by using the following equation reported by Bhattacharya et al [32]. …”
Section: Binding Constants Of the Thpp-hsa Complexmentioning
“…Like HP-HRP interaction, PP-HRP interaction is also entropy-driven process [24]. These features are expected for binding of ligands by primarily hydrophobic process [32]. Such hydrophobic interaction possibly involves an intermediate collisional process that becomes enhanced with rise in temperature and hence the binding constant increases with the increase of temperature.…”
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