Interaction of hyperhydricity‐preventing<i>pseudomonas</i>sp. with oregano (<i>origanum vulgare</i>) and selection of high phenolics and rosmarinic acid‐producing clonal lines

Eguchi, Yukiyo; Curtis, Otis F.; Shetty, Kalidas

doi:10.1080/08905439609549913

Cited by 39 publications

(27 citation statements)

References 33 publications

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“…It is also reported that RA biosynthesis was reduced after yeast elicitor treatment in Ti transformed Salvia miltiorrhiza cell suspension culture (Chen and Chen 2000) and after jasmonic acid treatment in hairy roots of Ocimum basilicum (Bais et al 2002). Similar results are noticed after Pseudomonas treatment of Thymus vulgaris (Kwok and Shetty 1996), Origanum vulgare (Eguchi et al 1996) and Rosmarinus officinalis (Yang et al 1997). Clones, tolerant to Pseudomonas, show reduction in RA accumulation, probably because of redirection of metabolism toward lignin synthesis (Yang et al 1997).…”

Section: Effects Of Elicitorssupporting

confidence: 76%

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

Bauer¹,

Kiseljak²,

Jelaska³

2009

Biologia plant.

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The leaves of axenically grown Coleus blumei were inoculated with Agrobacterium rhizogenes strain A4 and hairy root were established. PCR and Southern hybridization analysis confirmed transgenic nature of hairy root clones. Cultures of normal roots, induced by α-naphthaleneacetic acid on leaf explants, and hairy roots were evaluated for growth and rosmarinic acid content. Significantly better growth and up to 2.8 higher amount of rosmarinic acid was detected by HPLC analysis in hairy root clones. Methyl jasmonate stimulated rosmarinic acid accumulation in 6 out of 11 tested clones, while yeast extract induced RA accumulation in two and diminished it in 5 out of 11 tested hairy root clones.

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Section: Effects Of Elicitorssupporting

confidence: 76%

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

Bauer¹,

Kiseljak²,

Jelaska³

2009

Biologia plant.

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“…The in vitro shoot cultures were then vegetatively generated from a single seed originating phenotype under sterile conditions. The individual excised shoots of the clonal line were sub cultured on Murashige and Skoog (MS) hormone free medium (Murashige and Skoog 1962), containing MS basal salt mixture, Nitsch and Nitsch vitamins, 3% sucrose, and 0.3% gellan gum (Phytagel®) (Eguchi et al 1996). Different concentrations of NaCl (0, 10, 25, 50, 75, 100 mM) were added to the basal medium.…”

Section: Isolation Of Clonal Linementioning

confidence: 99%

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

et al. 2016

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Plants defense responses to abiotic stresses, including salinity stress, involve stimulation of defense related pathways such as biosynthesis of secondary metabolites and induction of endogenous antioxidant enzyme responses. In the present study, a single seed origin clonal line of Swertia chirayita inoculated with Lactobacillus plantarum (LP) was grown under different salinity levels. Control had no LP inoculation. S. chirayita inoculated with LP showed higher accumulation of proline, low proline dehydrogenase activity, up-regulation of pentose phosphate pathway, down-regulation of succinate dehydrogenase activity (Krebs cycle) and low total phenolic content with increased salt concentrations. In comparison, S. chirayita without LP adopted a different biochemical mechanism to counter salt stress (NaCl) by up-regulating both pentose phosphate pathway and Krebs cycle along with stimulation of phenolic biosynthesis. Guaiacol peroxidase (GPX) activity increased with and without LP treatment in response to increasing concentrations of salt. These results indicate that S. chirayita inoculated with LP exhibits a greater salinity stress tolerance than S. chirayita without LP by adopting a more energy efficient defense responses and potentially efficiently partitioning carbon flux between primary and secondary metabolism to counter salt induced oxidative stress.

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“…These two isomers are potent antimicrobial agents, having both antifungal and antibacterial activity in laboratory bioassays (Karamanoli et al, 2000;Vokou, 2007;Kadoglidou et al, 2011). Plants from two different oregano lines, GrO and O-5, were used, kindly provided by Ecopharm Hellas S.A. and the research team of Professor Kalidas Shetty, respectively; O-5 is a phenolic-rich oregano clone originated from a single heterozygous seed (Eguchi et al, 1996;Shetty et al, 1996).…”

Section: Plant Materialsmentioning

confidence: 99%

Are Leaf Glandular Trichomes of Oregano Hospitable Habitats for Bacterial Growth?

et al. 2012

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Phyllospheric bacteria were isolated from microsites around essential-oil-containing glands of two oregano (Origanum vulgare subsp. hirtum) lines. These bacteria, 20 isolates in total, were subjected to bioassays to examine their growth potential in the presence of essential oils at different concentrations. Although there were qualitative and quantitative differences in the essential oil composition between the two oregano lines, no differences were recorded in their antibacterial activity. In disk diffusion bioassays, four of the isolated strains could grow almost unrestrained in the presence of oregano oil, another five proved very sensitive, and the remaining 11 showed intermediate sensitivity. The strain least inhibited by oregano essential oil was further identified by complete16s rRNA gene sequencing as Pseudomonas putida. It was capable of forming biofilms even in the presence of oregano oil at high concentrations. Resistance of P. putida to oregano oil was further elaborated by microwell dilution bioassays, and its topology on oregano leaves was studied by electron microscopy. When inoculated on intact oregano plants, P. putida was able not only to colonize sites adjacent to essential oil-containing glands, but even to grow intracellularly. This is the first time that such prolific bacterial growth inside the glands has been visually observed. Results of this study further revealed that several bacteria can be established on oregano leaves, suggesting that these bacteria have attributes that allow them to tolerate or benefit from oregano secondary metabolites.

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Interaction of hyperhydricity‐preventingpseudomonassp. with oregano (origanum vulgare) and selection of high phenolics and rosmarinic acid‐producing clonal lines

Cited by 39 publications

References 33 publications

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

Are Leaf Glandular Trichomes of Oregano Hospitable Habitats for Bacterial Growth?

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