Interaction of neoplastic cells with glass surface under flow conditions

Doroszewski, J; Skierski, Janusz; Przaa̧dka, L.

doi:10.1016/0014-4827(77)90099-4

Cited by 36 publications

(9 citation statements)

References 9 publications

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“…HNSCC causes high morbidity due to its location, both through loss of function and disfigurement. Current treatments also commonly contribute to morbidity; extensive surgical resection can adversely affect speech and swallowing even when local cure is achieved, and the complications of radiotherapy such as xerostomia can be life-long [7,8].…”

Section: Introductionmentioning

confidence: 99%

Development of Microfluidic-based Analytical Methodology for Studying the Effects of Chemotherapy Agents on Cancer Tissue

Sylvester¹,

Hattersley²,

Stafford³

et al. 2013

Current Analytical Chemistry

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Whilst a multitude of techniques have been employed to study the biology of tumour tissue and its response to chemotherapeutic reagents, most current methodologies do not capture the sophistication of the in vivo environment. Microfluidics however offers the ability to maintain and interrogate primary tissue samples in an environment with biomimetic flow characteristics. In this study head and neck squamous cell carcinoma (HNSCC) tumour biopsies have been used to investigate the performance of a microfluidic device for generating clinically-useful information. The response of fresh and cryogenically-frozen primary HNSCC or metastatic lymph node samples to chemotherapy drugs (cisplatin, 5-flurouracil or docetaxel), alone and in combination, were monitored for both proliferation (water-soluble tetrazolium salt metabolism) and cell death biomarker release (lactate dehydrogenase, LDH) "off-chip". The frozen tissue showed no significant difference in terms of either proliferation or LDH release in comparison with the matched fresh samples. Administration of all drugs caused cell death, in a dose-response manner, with the combination showing the greatest amount of cytotoxicity particularly at days 8 and 9; correlating well with published clinical data. The system described here offers an innovative method for studying the tumour microenvironment in vitro and, through incorporation of relevant analytical modules, provides the basis of a pre-clinical device that can be used to define personalised treatment regimens.

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Section: Introductionmentioning

confidence: 99%

Development of Microfluidic-based Analytical Methodology for Studying the Effects of Chemotherapy Agents on Cancer Tissue

Sylvester¹,

Hattersley²,

Stafford³

et al. 2013

Current Analytical Chemistry

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“…Not only it is, perhaps, physiologically correct to culture cells in a flow, but it allows us to investigate phenomena (e.g., the rolling motion of leukocytes [22] or the migration of granulocytes [23]) whose mechanisms are still poorly understood. A number of flow chambers have been developed through the years, including parallel-plate flow chambers and stagnation-point flow chambers, that have been used to study, among others, cell adhesion, bacterial adhesion, microsphere deposition, and receptor-ligand bonds [18], [19], [21], [22], [24]- [28]; in a parallel-plate flow chamber, the motion of a sphere in laminar flow can be predicted with high approximation, and cell trajectories, speed, and adhesion can be monitored.…”

Section: Introductionmentioning

confidence: 99%

A Parallel-Plate Flow Chamber to Study Initial Cell Adhesion on a Nanofeatured Surface

Martines

Mcghee²,

Wilkinson³

et al. 2004

IEEE Trans.on Nanobioscience

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Abstract-Cells in the human body come across many types of information, which they respond to. Both material chemistry and topography of the surface where they adhere have an effect on cell shape, proliferation, migration, and gene expression. It is possible to create surfaces with topography at the nanometric scale to allow observation of cell-topography interactions. Previous work has shown that 100-nm-diameter pits on a 300-nm pitch can have a marked effect in reducing the adhesion of rat fibroblasts in static cultures. In the present study, a flow of cell suspension was used to investigate cell adhesion onto nanopits in dynamic conditions, by means of a parallel-plate flow chamber. A flow chamber with inner nanotopography has been designed, which allows real-time observation of the flow over the nanopits. A nanopitted pattern was successfully embossed into polymethylmethacrylate to meet the required shape of the chamber. Dynamic cell adhesion after 1 h has been quantified and compared on flat and nanopitted polymethylmethacrylate substrates. The nanopits were seen to be significantly less adhesive than the flat substrates ( 0 001), which is coherent with previous observations of static cultures.

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“…Quantitative adhesion assays have been developed to apply controlled detachment forces through centrifugation (21), hydrodynamic fluid flow (22)(23)(24)(25)(26)(27)(28)(29)(30), or micromanipulation (31)(32)(33). Although these methods have provided measurements of adhesion strength, the functional dependence of adhesion strength on receptor-ligand parameters remains unknown.…”

mentioning

confidence: 99%

Force Required to Break α5β1Integrin-Fibronectin Bonds in Intact Adherent Cells Is Sensitive to Integrin Activation State

Garcı́a

Huber

Boettiger

1998

Journal of Biological Chemistry

142

173

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Interaction of neoplastic cells with glass surface under flow conditions

Cited by 36 publications

References 9 publications

Development of Microfluidic-based Analytical Methodology for Studying the Effects of Chemotherapy Agents on Cancer Tissue

Development of Microfluidic-based Analytical Methodology for Studying the Effects of Chemotherapy Agents on Cancer Tissue

A Parallel-Plate Flow Chamber to Study Initial Cell Adhesion on a Nanofeatured Surface

Force Required to Break α5β1Integrin-Fibronectin Bonds in Intact Adherent Cells Is Sensitive to Integrin Activation State

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