Interaction of Osteoblasts with Macroporous Scaffolds Made of PLLA/PCL Blends Modified with Collagen and Hydroxyapatite

Aydin, Halil Murat; Yang, Ying; Köhler, Thomas; Haj, Alicia J. El; Müller, Ralph; Pi̇şki̇n, Erhan

doi:10.1002/adem.200900030

Cited by 17 publications

(11 citation statements)

References 31 publications

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“…Our groups are interested in using these biodegradable polyesters to prepare tissue engineering scaffolds. [38][39][40] Therefore, we have sought SBs that are degradable and safe for medical use to polymerize LLA and e-CL in scCO 2 . In this study, we synthesized triblock copolymers of PEG, which is one of the safest water-soluble polymers used in various medical applications, and e-CL, which is one of the co-monomers used in the final polymer product.…”

Section: Introductionmentioning

confidence: 99%

Ring-opening copolymerization of L-lactide and ɛ-caprolactone in supercritical carbon dioxide using triblock oligomers of caprolactone and PEG as stabilizers

Yılmaz

Eğri

Yıldız

et al. 2011

Polym J

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In this study, the use of triblock (A-B-A) oligomers of e-caprolactone (e-CL) (A) and PEG400 (B) as stabilizers (SB) for the copolymerization of L-lactide (LLA) and e-CL in supercritical carbon dioxide (scCO 2 ) was investigated. To determine the effect of CO 2 -philic and polymer-philic segments on copolymerization, oligomers with three different average molecular weights (M w ¼2000-6000 Da) were synthesized by changing the PEG400/e-CL ratio. Copolymerizations were confirmed by 1 H-nuclear magnetic resonance (NMR), 13 C-NMR and differential scanning calorimeter data. It was possible to copolymerize LLA and e-CL in scCO 2 without any SB; however, the polymerization yields and average molecular weights were low, and significant aggregate formations were detected. Recipes featuring only 5% SB were successfully applied to reach high polymerization yields of B85% and polymers with average molecular weights greater than 20 kDa. When the polymer-philic segment of the SB increased, both the yield and molecular weight of the copolymer also increased significantly, resulting in white powdery products.

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Section: Introductionmentioning

confidence: 99%

Ring-opening copolymerization of L-lactide and ɛ-caprolactone in supercritical carbon dioxide using triblock oligomers of caprolactone and PEG as stabilizers

Yılmaz

Eğri

Yıldız

et al. 2011

Polym J

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“…with the molecular weight of 200 kDa and 40 kDa respectively [4]. The blend solution was made by dissolution the polymer in chloroform.…”

Section: Methodsmentioning

confidence: 99%

Assessment of a new biomimetic scaffold and its effects on bone formation by OCT

Yang

Aydın

Pi̇şki̇n

et al. 2009

Optics in Bone Biology and Diagnostics

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The ultimate target of bone tissue engineering is to generate functional load bearing bone. By nature, the porous volume in the trabecular bone is occupied by osseous medulla. The natural bone matrix consists of hydroxyapatite (HA) crystals precipitated along the collagen type I fibres. The mineral phase renders bone strength while collagen provides flexibility. Without mineral component, bone is very flexible and can not bear loads, whereas it is brittle in the case of mineral phase without the collagen presence. In this study, we designed and prepared a new type of scaffold which mimics the features of natural bone. The scaffold consists of three different components, a biphasic polymeric base composed of two different biodegradable polymers prepared by using dual porogen approach and bioactive agents, i.e., collagen and HA particles which are distributed throughout the matrix only in the pore surfaces. Interaction of the bioactive scaffolds possessing very high porosity and interconnected pore structures with cells were investigated in a prolonged culture period by using an osteoblastic cell line. The mineral HA particles have a slight different refractive index from the other elements such as polymeric scaffolds and cell/matrix in a tissue engineering constructs, exhibiting brighter images in OCT. Thus, OCT renders a convenient means to assess the morphology and architecture of the blank biomimetic scaffolds. This study also takes a close observation of OCT images for the cultured cell-scaffold constructs in order to assess neo-formed minerals and matrix. The OCT assessments have been compared with the results from confocal and SEM analysis.

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“…The favorable properties of these polymers include good biocompatibility, biodegradability, and ease of handling. [116][117][118] Some other very rarely studied synthetic polymers for potential biomedical sciences are poly(carbonate urethane), [119] poly(acrylic acid), [92] poly(l-lysine), [120] and elastomers. [121][122][123] 2.2.1.…”

Section: Synthetic Polymersmentioning

confidence: 99%

“…[138] PCL can be blended or co-polymerized with other polymers, such as PLA or PLGA in order to improve its physical properties. [116] GO/PCL nanocomposites were prepared in the form of rods and electrospun mats.…”

Section: Pclmentioning

confidence: 99%

Graphene Oxide/Polymer‐Based Biomaterials

2017

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Since its discovery in 2004, derivatives of graphene have been developed and heavily investigated in the field of tissue engineering. Among the most extensively studied forms of graphene, graphene oxide (GO), and GO/ polymer-based nanocomposites have attracted great attention in various forms such as films, 3D porous scaffolds, electrospun mats, hydrogels, and nacre-like structures. In this review, the most actively investigated GO/ polymer nanocomposites are presented and discussed, these nanocomposites are based on chitosan, cellulose, starch, alginate, gellan gum, poly(vinyl alcohol) (PVA), poly(acrylamide), poly(e-caprolactone) (PCL), poly(lactic acid) (PLLA), poly(lactide-co-glycolide) (PLGA), gelatin, collagen, and silk fibroin (SF). The biological and mechanical performance of such nanocomposites are comprehensively scrutinized and ongoing research questions are addressed. The analysis of the literature reveals overall the great potential of GO/ polymer nanocomposites in tissue engineering strategies and indicates also a series of challenges requiring further research efforts.

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Interaction of Osteoblasts with Macroporous Scaffolds Made of PLLA/PCL Blends Modified with Collagen and Hydroxyapatite

Cited by 17 publications

References 31 publications

Ring-opening copolymerization of L-lactide and ɛ-caprolactone in supercritical carbon dioxide using triblock oligomers of caprolactone and PEG as stabilizers

Ring-opening copolymerization of L-lactide and ɛ-caprolactone in supercritical carbon dioxide using triblock oligomers of caprolactone and PEG as stabilizers

Assessment of a new biomimetic scaffold and its effects on bone formation by OCT

Graphene Oxide/Polymer‐Based Biomaterials

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