Interaction of photosynthetic bacterium, <i>Rhodopseudomonas Palustris</i>, with montmorillonite clay

Abstract

In this study, we investigate the interaction of Rhodopseudomonas Palustris (R. Palustris) with montmorillonite clay. The adsorption of bacteria on the clay surface was also determined as a function of the initial bacterial quantity, pH, temperature and ionic strength. At different initial bacterial quantities, the percentage of bacteria adsorbed ranged from 61.07% to 77.57%, and the higher percentage was determined to be 15.0×108 cfu ml -1 . In addition, the actual number of adsorbed cells was significantly correlated with the initial quantity of R. Palustris. A greater degree of R. Palustris adsorption on the montmorillonite was observed in the temperature range of 30oC to 40oC. It was also found that as the pH and ionic strength increased the percentage of bacterial adsorption on montmorillonite decreased. There were no significant differences (P > 0.05) in the enzyme activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) between the bacteria treated with and without montmorillonite. However, montmorillonite supplementation significantly increased (P < 0.05) the cell surface hydrophobicity of R. Palustris compared with the control. These results indicated that the interaction was controlled by physicochemical characteristics between cells and the mineral substrate.

Effects of polyethoxylate lauryl ether (Brij 35) addition on phenanthrene biodegradation in a soil/water system

“…In addition, a high correlation (R 2 D 0.89) has been found between strong adsorption and the clay content of soil. [39,40] Strong adsorption of Pseudomonas putida is known to occur with clay minerals, including montmorillonite, kaolinite and goethite. The soil-attached bacteria must arise by the adsorption of free-living bacterial onto the surface of soil particles in the clay/water system.…”

Natural and Synthetic Nanomaterials in Microbial Biotechnologies for Crop Production

“…Microbes, especially bacteria, affect the kinetics and course of reactions including the formation of many minerals in the lithosphere and hydrosphere of the Earth [49][50][51]. On the other hand, minerals strongly affect the survival of microorganisms, their physiological and biochemical activity [52][53].…”

Macro- to Nanoscale Heat and Mass Transfer: The Lagging Behavior

“…The transient temperature field around a thermally insulated crack in a substrate bonded to a coating is obtained using the hyperbolic heat conduction model [287,288]. The hyperbolic, non-Fourier effects for a crack in the interface of a layered medium of finite thickness with the emphasis on the solution technique for the crack tip thermal flow field associated with the Griffith and penny-shaped cracks are studied [289,290].…”