Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis

Li, Rugang; Rimmer, Roger; Buchwaldt, L.; Sharpe, Andrew; Séguin-Swartz, G.; Coutu, Cathy; Hegedus, Dwayne D.

doi:10.1016/j.fgb.2004.03.001

Cited by 70 publications

(58 citation statements)

References 65 publications

(65 reference statements)

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“…The expressed sequence tag (EST) sequences obtained were compared to GenBank dbEST database and non-redundant protein database for a homology search, and analyses were conducted using the BLAST program of the National Center of Biotechnology Information (NCBI; http:// www.ncbi.nlm.nih.gov). The evaluation rule of significant sequences was E-value less than 10 -5 and identities more than 40% (Li et al, 2004). The function of ESTs was classified according to the method of Bevan et al (1998).…”

Section: Sequencing and Bioinformatic Analysismentioning

confidence: 99%

Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308

Alam

Zhai

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Powdery mildew, caused by Blumeria graminis f. sp tritici (Bgt) is one of the devastating diseases of wheat and causes yield losses in temperate wheat growing regions. A wheat line, N0308 with resistance to powdery mildew was used in this study. A suppression subtractive hybridization cDNA library was constructed from the wheat leaves inoculated by Bgt at the two-leaf stage. The differentially expressed genes in response to Bgt infection in wheat were identified, and a total of 175 positive clones from the library were sequenced, and 90 expressed sequence tags (ESTs) were subjected to clustering, BLAST alignment, functional annotation, and classification into different categories. By comparing the EST sequences among the SSH-cDNA libraries, we analyzed gene expression patterns of 7 ESTs associated with the resistance reaction of powdery mildew by using semi-quantitative reverse transcription-polymerase chain reaction. The expression of 5 genes (sulfatase, pathogenesis-related protein 17, betacarbonic anhydrase 2, thioredoxin h-like protein, and coronatineinsensitive) transcripts was induced, and the transcript levels of these genes were the highest at 72 h after Bgt infection, while those of 2 genes (violaxanthin de-epoxidase and gag-pol-polyprotein) were the highest level at 12 and 18 h post-infection, respectively. These findings suggest that these genes are induced at an early stage of infection and are transcriptionally activated for the host defense response.

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Section: Sequencing and Bioinformatic Analysismentioning

confidence: 99%

Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308

Alam

Zhai

et al. 2014

Genet. Mol. Res.

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“…Based on the expressed sequence tag (EST) approach proteases have been identified from S. sclerotiorum libraries. cDNAs encoding an acid protease and two aspartic proteases were identified in the infected Brassica napus stem library (Li et al, 2004). Also, cDNAs encoding an aspartyl protease was found only in the infection cushions and a serine protease in libraries prepared from mycelia grown on agar.…”

Section: The Effect Of Ph and Inhibitors On Protease Activitymentioning

confidence: 99%

Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection

Bueno

Oliveira²,

Andrade³

et al. 2012

Genet. Mol. Res.

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“…Recently, molecular biological approaches such as differential screening, expressed sequencing tags (EST), and DNA microarray have been used to identify inducible genes in response to various stresses from many plant tissues, including nonwoody plants (Arabidopsis, tomato, Brassica) [18][19][20] and woody plants (poplar, grapefruit). 21,22) In tea leaves (C. sinensis), Chen et al 23) performed EST analysis of spring tender shoots.…”

mentioning

confidence: 99%

Chemical Profiling and Gene Expression Profiling during the Manufacturing Process of Taiwan Oolong Tea “Oriental Beauty”

Cho

Mizutani

Shimizu

et al. 2007

Bioscience, Biotechnology, and Biochemistry

101

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Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis

Cited by 70 publications

References 65 publications

Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308

Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308

Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection

Chemical Profiling and Gene Expression Profiling during the Manufacturing Process of Taiwan Oolong Tea “Oriental Beauty”

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