Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphogenetic protein-1

Müller, Wernér E.G.; Binder, Michael; Lintig, Johannes von; Guo, Yugene; Wang, Xiaohong; Kaandorp, Jaap A.; Wiens, Matthias; Schröder, Heinz C.

doi:10.1016/j.bbagen.2011.09.006

Cited by 29 publications

(35 citation statements)

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“…These strains were subsequently additionally transformed with the S. domuncula SDrBCO gene inserted into pBAD-TOPO . As a positive control, the carotinoid synthesizing bacteria were transformed with SDCDO [16]. The bacteria, lacking the sponge oxygenase genes, either SDrBCO or SDCDO , termed E. coli (−) , turned to a yellowish (for E. coli ( β-car+ ) ) or reddish color (for E. coli ( lyco+ ) ) due to the accumulation of β-carotene or lycopene, respectively (Figure 7).…”

Section: Resultsmentioning

confidence: 99%

“…In the present study a novel strategy has been detected in sponges by which a toxic peptide remains functionally inactive for the host producer, by the interaction of the peptide with a low molecular bioactive metabolite. Following our earlier finding that the demosponge S. domuncula is rich in retinoids [15], we elucidated recently the basic pathway of the retinoids in this species [16]. The retinoid β-carotene is produced by bacteria that are symbiotically associated with this sponge.…”

Section: Introductionmentioning

confidence: 97%

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Differential Expression of the Demosponge (Suberites domuncula) Carotenoid Oxygenases in Response to Light: Protection Mechanism Against the Self-Produced Toxic Protein (Suberitine)

et al. 2012

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Abstract:The demosponge Suberites domuncula has been described to contain high levels of a proteinaceous toxin, Suberitine, that displays haemolytic activityIn the present study this 7-8 kDa polypeptide has been isolated and was shown to exhibit also cytotoxic effects on cells of the same species. Addition of retinal, a recently identified metabolite of β-carotene that is abundantly present in S. domuncula was found to reduce both the haemolytic and the cell toxic activity of Suberitine at a molar ratio of 1:1. Spectroscopic analyses revealed that the interaction between β-carotene and Suberitine can be ascribed to a reversible energy transfer reaction. The enzyme that synthesises retinal in the sponge system is the β,β-carotene-15,15′-dioxygenase [carotene dioxygenase]. In order to clarify if this enzyme is the only β-carotene-metabolizing enzyme a further oxygenase had been identified and cloned, the (related) carotenoid oxygenase. In contrast to the dioxygenase, the carotenoid oxygenase could not degrade β-carotene or lycopene in Escherichia coli strains that produced these two carotenoids; therefore it had been termed related-carotenoid oxygenase. Exposure of primmorphs to light of different wavelengths from the visible OPEN ACCESSMar. Drugs 2012, 10 178 spectrum resulted after 3 days in a strong upregulation of the dioxygenase in those 3D-cell aggregates that had been incubated with β-carotene. The strongest effect is seen with blue light at a maximum around 490 nm. It is concluded that the toxin Suberitine is non-covalently modified by retinal, the cleavage product from β-carotene via the enzyme carotene dioxygenase, a light inducible oxygenase. Hence, this study highlights that in S. domuncula the bioactive metabolite, retinal, has the property to detoxify its homologous toxin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Differential Expression of the Demosponge (Suberites domuncula) Carotenoid Oxygenases in Response to Light: Protection Mechanism Against the Self-Produced Toxic Protein (Suberitine)

et al. 2012

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“…In addition, acetylenic carotenoid sulfates termed bastaxanthins, and other related compounds thought to be fucoxanthin metabolites derived from microalgae [69] , have been isolated from the marine sponges Ianthella basta [52] and Prianos osiros [70] . Recent molecular studies aimed at elucidating the main elements of the retinoid metabolic pathway in the demosponge S. domuncula revealed that the retinoid precursor b-carotene, which is enzymatically cleaved via the enzyme b-b-carotene 15,15'-dioxygenease to generate retinal, is generated by bacteria that form a symbiotic association with this sponge [71,72] .…”

Section: Carotenoids (C 40 )mentioning

confidence: 99%

Novel natural product discovery from obligate symbiotic organisms of marine sponges

Monaco

Quinlan

2018

J Nat Agents Mol Ther

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A rich source for the discovery of novel, pharmacologically active natural products have been the terrestrial plants and microbes, accounting for about 85% of the approved natural products in pharmaceutical use, and about 60% of approved pharmaceuticals and new drug applications submission annually. Discovery in the marine environment has lagged due to the difficulty of exploration in this ecological niche. Such exploration began in the 1950's, after technological advances such as scuba diving which allowed collection of marine organisms, primarily to a depth of about 15 m, which was the limit of that technology. Natural products from filter feeding marine invertebrates and in particular, sponges, have proven to be a rich source of structurally unique pharmacologically active compounds, with over 16,000 molecules isolated thus far and a continuing pace of discovery at hundreds of novel bioactive molecules per year. All classes of pharmaceuticals have been represented in this discovery process, including antiprotozoals, pesticides, TGF-beta inhibitors, cationic channel blockers, anticancer, cytotoxic, antiviral, anti-inflammatory and antibacterial compounds. Important biosynthetic pathways found in sponges which give rise to these compounds include the terpenoid, fatty acid, polyketoid, quinone reductase, alkaloid, isoprenoid, and non-ribosomal protein synthase pathways as summarized in this article.

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“…In addition, acetylenic carotenoid sulfates termed bastaxanthins, and other related compounds thought to be fucoxanthin metabolites derived from microalgae, have been isolated from the marine sponges Ianthella basta [98] and Prianos osiros [70]. Recent molecular studies aimed at elucidating the main elements of the retinoid metabolic pathway in the demosponge S. domuncula revealed that the retinoid precursor β-carotene, which is enzymatically cleaved via the enzyme β-β-carotene 15,15'-dioxygenease to generate retinal, is generated by bacteria that form a symbiotic association with this sponge [71,72].…”

Section: Cc-by-nd 40 International License Peer-reviewed) Is the Autmentioning

confidence: 99%

Novel Natural Product Discovery from Marine Sponges and their Obligate Symbiotic Organisms

Monaco

Quinlan

2014

Preprint

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Discovery of novel natural products is an accepted method for the elucidation of pharmacologically active molecules and drug leads. Best known sources for such discovery have been terrestrial plants and microbes, accounting for about 85% of the approved natural products in pharmaceutical use (1), and about 60% of approved pharmaceuticals and new drug applications annually (2). Discovery in the marine environment has lagged due to the difficulty of exploration in this ecological niche. Exploration began in earnest in the 1950's, after technological advances such as scuba diving allowed collection of marine organisms, primarily at a depth to about 15m. Natural products from filter feeding marine invertebrates and in particular, sponges, have proven to be a rich source of structurally unique pharmacologically active compounds, with over 16,000 molecules isolated thus far (3, 1) and a continuing pace of discovery at hundreds of novel bioactive molecules per year. All classes of pharmaceuticals have been represented in this discovery process, including antiprotazoals, pesticides, TGF-beta inhibitors, cationic channel blockers, anticancer, cytotoxic, antiviral, anti-inflammatory and antibacterial compounds. Important biosynthetic pathways found in sponges which give rise to these compounds include the terpenoid (4), fatty acid, polyketoid, quinone reductase, alkaloid, isoprenoid (5), and non-ribosomal protein synthase pathways.

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Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphogenetic protein-1

Cited by 29 publications

References 96 publications

Differential Expression of the Demosponge (Suberites domuncula) Carotenoid Oxygenases in Response to Light: Protection Mechanism Against the Self-Produced Toxic Protein (Suberitine)

Differential Expression of the Demosponge (Suberites domuncula) Carotenoid Oxygenases in Response to Light: Protection Mechanism Against the Self-Produced Toxic Protein (Suberitine)

Novel natural product discovery from obligate symbiotic organisms of marine sponges

Novel Natural Product Discovery from Marine Sponges and their Obligate Symbiotic Organisms

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