Interaction of two hormones and their effect on observed rate of initiation of DNA synthesis in 3T3 cells

Asúa, Luis Jiménez de; O'Farrell, M.; Bennett, Dorothy C.; Clingan, Dorothie; Rudland, Philip S.

doi:10.1038/265151a0

Cited by 56 publications

(5 citation statements)

References 17 publications

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“…However, whole blood samples, crude platelet lysates, and tissue biopsies contain a variety of agents other than PDGF which stimulate replicative DNA synthesis and cell division in cultured fibroblasts. These include epidermal growth factor (12), prostaglandins (13), insulin (14), and somatomedins (15). Thus, there have been no unambiguous estimates of the PDGF content of common biological fluids such as blood or platelet-poor plasma.…”

mentioning

confidence: 99%

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Singh

Chaikin

Stiles

1982

The Journal of Cell Biology

105

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Competition between 125I-labeled platelet-derived growth factor (PDGF) and unlabeled PDGF forms the basis of a specific "radio-receptor assay" for quantifying PDGF in clotted blood serum. Human clotted blood serum contains 15 ng/ml of PDGF by radio-receptor assay; this corresponds to a PDGF content of approximately 7.5 x 10(-5) pg per circulating platelet, a figure which is corroborated by purification data. Clotted blood sera from mammals, lower vertebrates and marine invertebrates were screened for homologues of human PDGF by radio-receptor assay. All tested specimens from phylum Chordata contain a mitogenic agent that competes with human PDGF for receptor binding. Sera from tunicates down on the chordate line of evolution and sera from all tested animals on the arthropod line of development were negative. The phylogenetic distribution of PDGF homologue does not correlate with platelet distribution since platelets and their precursor cell--the bone marrow megacaryocyte--are unique to the mammalian hematopoietic system. One anatomical feature appearing coordinately with PDGF on the vertebrate line of development is a pressurized circulatory system. The coincidental appearance of these features may lend support to the hypothesis that PDGF plays a role in maintenance and repair of the vascular lining in vivo.

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mentioning

confidence: 99%

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Singh

Chaikin

Stiles

1982

The Journal of Cell Biology

105

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“…The effects of TGFj?l, insulin and PGF2a on 2-deoxyglucose (DOG) uptake induction [13] after 6 h are shown in Table 2. Both TGFBl (0.8 &ml) and PGF2a (300 &ml) increased glucose uptake fivefold.…”

Section: Insulin and Tgfj31 Increase Pgfz'a-induced Dog Uptakementioning

confidence: 99%

Transforming growth factor β1, insulin and prostaglandin E₁ enhance prostaglandin F_2α mitogenic action in Swiss 3T3 cells via separate events

et al. 1994

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Transforming growth factorb (TGFBl) had no mitogenic effect in Swiss 3T3 cells, but could increase prostaglandin F, (PGFZa)-induced DNA synthesis. Insulin, but not prostaglandin E, (PGEl), further enhanced PGF2cr action at low TGFpl concentrations. TGFBl also acted concertedly with the protein kinase C (PKC) activator I-oleoyl-2-acetylglycerol to induce mitogenesis. Thus, it appears that TGFBl and insulin act via separate signals, while TGFBl and PGEl might share a common pathway not involving TGF/?l-mediated prostaglandin synthesis. These results suggest that TGFBl might elicit various signalling mechanisms to enhance PGFZa-triggered events.

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“…In fact, this depression in "saturation density" is not unlike the depression in maximum NE-dependent activity at nonoptimal serum concentrations we report. Thus, it seems that in vitro synergistic action of hormones and factors are common [Barnes and Sato, 1980;Bottenstein et al, 1979;De Asua et al, 1977;Ham and Mc Keehan, 1979;Smith and Temin, 19741. Synergistic effects with serum were demonstrated in studies with a factor from beta-globulin [Holley and Kiernan, 19711, with epidermal growth factor [Carpenter and Cohen, 19781, and with vasopressin [Rozengurt et al, 19791. Besides depressed activities due to nonoptimum serum concentrations, decreasing the concentration of a nutrient such as thymidine caused a similar depression in NEdependent activity, In this experiment, the serum concentration was held at its optimum 5%, while thymidine was omitted from the medium and muscle cells were grown in the presence of 1 FCi/ml of tritiated thymidine, only 0.43% of the normal thymidine concentration.…”

Section: Effects Of Nonoptimum Serum Concentrations On Ne-dependent Amentioning

confidence: 99%

Dose‐dependent initiation of myogenesis by neurotrophic factor

Popiela

Ellis

Festoff

1982

J of Neuroscience Research

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The kinetics of primary chick muscle cells in response to partially purified neurotrophic factor (NTF) were investigated. Chick muscle cells are routinely plated in the absence of NTF but in the presence of horse serum to allow cell attachment to the substratum; a maximum number of cells attaches 5-8 hr after plating, but cells do not proliferate in the absence of NTF, or do so extremely slowly. Subsequent to a routine attachment period of 6 hr, the medium is aspirated and replaced with medium containing 2-20 micrograms/ml of NTF as well as horse serum. Dose responsiveness at elevated NTF concentrations is observed only if F12 medium, supplemented with serum, insulin, transferrin, and selenium is used. In the presence of commercial F10, although supplemented with serum, dose responsiveness is not apparent at more than 2 micrograms/ml of NTF. Upon exposure to NTF, in the presence of supplemented F12 and serum, an 8-hr lag ensues; then, thymidine is incorporated at NTF dose-dependent rates by growing cell populations. The rates of incorporation into cell populations depend on the initial number of cells plated and the amount of NTF supplied. Cell counting indicates that dose-dependent proliferation of muscle cells has occurred during the first 48 hr of exposure to NTF. The specific activity of NTF is repeatedly shown to be at least 10-fold greater than unfractionated nerve extract. Shortly after 48 hr of exposure to NTF, myoblasts characteristically begin to fuse with one another, and myogenesis is visible. These studies show that the initiation of in vitro myogenesis in primary chick muscle cells depends on the amount of NTF supplied. The work also indicates a requirement for optimum medium conditions in order to detect dose-responses to NTF.

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Interaction of two hormones and their effect on observed rate of initiation of DNA synthesis in 3T3 cells

Cited by 56 publications

References 17 publications

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Transforming growth factor β1, insulin and prostaglandin E₁ enhance prostaglandin F_2α mitogenic action in Swiss 3T3 cells via separate events

Dose‐dependent initiation of myogenesis by neurotrophic factor

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Interaction of two hormones and their effect on observed rate of initiation of DNA synthesis in 3T3 cells

Cited by 56 publications

References 17 publications

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.

Transforming growth factor β1, insulin and prostaglandin E1 enhance prostaglandin F2α mitogenic action in Swiss 3T3 cells via separate events

Dose‐dependent initiation of myogenesis by neurotrophic factor

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Transforming growth factor β1, insulin and prostaglandin E₁ enhance prostaglandin F_2α mitogenic action in Swiss 3T3 cells via separate events