Interactions of drugs and an oligonucleotide with charged membranes analyzed by immobilized liposome chromatography

Abstract

We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or distearoyltrimethylammoniumpropane) or detergent (sodium dodecylsulfate) interacted electrostatically in a concentration-dependent matter with the solutes. The oligonucleotide ions presumably bound to the liposomes by multipoint interactions, which was saturable. Sodium dodecylsulfate seemed to affect the drug-membrane interactions more strongly than phosphatidylserine did, probably due to different positioning in the bilayer.

Bilayer disk capillary electrophoresis: A novel method to study drug partitioning into membranes

“…As expected [3,10], cationic drugs partitioned well into PEG-stabilized disks (Table 1) with the expected linear relation between lipid load and migration time (Figs. 2 and 3).…”

“…2 and 3). The solute partitioning into disks stabilized with PEG-DSPE(5000) was generally more pronounced than the partitioning into disks stabilized with PEG-Cer(5000), which indicates the importance of electrostatic interactions [3,6,8] in addition to the hydrophobic interactions [2,28]. Furthermore, the variation in the partitioning between different disk systems appeared to depend on the position and orientation of the charged/polar groups of the drugs (Table 1).…”

“…They are commonly used as model membranes in studies of substance-membrane interactions [1][2][3] and as carriers of drugs to be released at specific targets in the body [4]. The partitioning of solutes into liposomes has been studied by several analytical techniques such as immobilized liposome chromatography (ILC) [5][6][7][8], isothermal titration calorimetry [9,10], and CE with liposomes as a pseudostationary phase (liposome EKC) [1,11] or as a stationary phase [12,13].…”

Preparation and characterization of phosphatidylcholine‐coated zirconia–magnesia stationary phase for artificial membrane chromatography

“…The other two types (immobilized liposome and artificial membrane) not only simulate structure of real membrane but also have some advantages, i.e . good stability and easy to be prepared, so that they have been used in wide fields of screening drug 9–13, studying affinity characteristics of proteins on membrane, investigating interaction between peptides (or proteins) and membrane 14–17, and separating membrane proteins 18–20, nuclei acids and isomers 1, 21.…”