Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA

Wood, Margaret M.; Rajeev, Lara; Gardner, Jeffrey F.

doi:10.1128/jb.01011-13

Cited by 6 publications

(4 citation statements)

References 44 publications

(74 reference statements)

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“…For prophages and ICEs encoding a tyrosine integrase, a recombination directionality factor (RDF) encoded by the element, also known as excisionase, generally helps to reverse the direction of the recombination towards excision [ 57 ]. Such RDFs have also been identified (or proposed) for most IMEs encoding tyrosine integrases [ 26 , 38 , 41 , 58 , 59 , 60 ]. Besides a RDF and a tyrosine integrase, an additional protein encoded by the IME Tn 4555 is required for its optimal excision from Bacteroides fragilis genome [ 59 ].…”

Section: Maintenance Of Imesmentioning

confidence: 99%

“…All these IMEs are able to integrate in the chromosome of their natural hosts in the absence of the helper elements. However, none of them is able to excise in their absence since the main regulators of the helper elements are required for the expression of the RDF genes carried by the IMEs [ 25 , 60 ]. For example, the SXT-encoded transcriptional activators SetCD trigger the expression of genes encoding the integrase and the RDF of MGI Vfl Ind1, promoting its site-specific excision [ 58 ].…”

Section: Maintenance Of Imesmentioning

confidence: 99%

See 1 more Smart Citation

The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems

Guédon

Libante

Coluzzi

et al. 2017

Genes

105

112

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Conjugation is a key mechanism of bacterial evolution that involves mobile genetic elements. Recent findings indicated that the main actors of conjugative transfer are not the well-known conjugative or mobilizable plasmids but are the integrated elements. This paper reviews current knowledge on “integrative and mobilizable elements” (IMEs) that have recently been shown to be highly diverse and highly widespread but are still rarely described. IMEs encode their own excision and integration and use the conjugation machinery of unrelated co-resident conjugative element for their own transfer. Recent studies revealed a much more complex and much more diverse lifecycle than initially thought. Besides their main transmission as integrated elements, IMEs probably use plasmid-like strategies to ensure their maintenance after excision. Their interaction with conjugative elements reveals not only harmless hitchhikers but also hunters that use conjugative elements as target for their integration or harmful parasites that subvert the conjugative apparatus of incoming elements to invade cells that harbor them. IMEs carry genes conferring various functions, such as resistance to antibiotics, that can enhance the fitness of their hosts and that contribute to their maintenance in bacterial populations. Taken as a whole, IMEs are probably major contributors to bacterial evolution.

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Section: Maintenance Of Imesmentioning

confidence: 99%

Section: Maintenance Of Imesmentioning

confidence: 99%

The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems

Guédon

Libante

Coluzzi

et al. 2017

Genes

105

112

View full text Add to dashboard Cite

show abstract

“…The intN1 was identified as an integrase of the non-replicative Bacteroides unit 1 (NBU1) (Rajeev et al, 2006, 2007). The excision of NBU1 is a complex process and needs other proteins that are involved in transposon interaction with intN1 (Wood et al, 2013). In contrast, the excision of the 10K GI did not need GI encoded proteins.…”

Section: Discussionmentioning

confidence: 99%

Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

et al. 2019

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Riemerella anatipestifer is a Gram-negative, pathogenic bacterium, which is harmful to poultry. However, the genomic islands (GIs) in R. anatipestifer are not well-studied. In this study, a 10K genomic island was predicted by the bioinformatics analysis of R. anatipestifer ATCC 11845, which is widely found in other R. anatipestifer genomes. We had first reported the genomic island integration and excision function in R. anatipestifer. We successfully constructed the integration plasmid by using the integrase and 53 bp attP elements. The 10K GI was found integrated at the 53 bp attB located in the Arg-tRNA of the R. anatipestifer RA-YM chromosome. We identified an integrase that helped in the precise integration and excision in R. anatipestifer and elucidated the molecular mechanism of the 10K genomic island integration and excision. Furthermore, we provided a new method for the gene expression and construction of complementary strain.

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“…Footprinting reactions were performed as described previously (17). Footprinting primers (attDOT top and bottom strands) were purchased from Integrated DNA Technologies, with 6-carboxyfluorescein (6-FAM) at the 5= end.…”

Section: Media and Antibioticsmentioning

confidence: 99%

The Bacteroides thetaiotaomicron Protein Bacteroides Host Factor A Participates in Integration of the Integrative Conjugative Element CTnDOT into the Chromosome

Ringwald

Gardner

2015

J Bacteriol

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CTnDOT is a conjugative transposon found in Bacteroides species. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroides host factor A), that participates in integrative recombination. BHFa is the first host factor identified for a site-specific recombination reaction in the CTnDOT family of integrative and conjugative elements. Based on the amino acid sequence of BHFa, the ability to bind specifically to 4 sites in the attDOT DNA, and its activity in the integration reaction, BHFa is a member of the IHF/HU family of nucleoid-associated proteins. Other DNA bending proteins that bind DNA nonspecifically can substitute for BHFa in the integration reaction. IMPORTANCEBacteroides species are normal members of the human colonic microbiota. These species can harbor and spread self-transmissible genetic elements (integrative conjugative elements [ICEs]) that contain antibiotic resistance genes. This work describes the role of a protein, BHFa, and its importance in the integration reaction required for the element CTnDOT to persist in Bacteroides host cells. Bacteroides species are Gram-negative, obligate anaerobes that are part of the normal microbiota in the human colon (1). When the gut is punctured, Bacteroides can act as an opportunistic pathogen that may form abscesses in other regions of the body. Treatment of the abscesses is complicated by widespread resistance to tetracycline and erythromycin carried by integrative conjugative elements (ICEs) (also called conjugative transposons [CTns]) found in Bacteroides. These elements are capable of transferring between diverse organisms by conjugation and integrate into the host bacterium's chromosome. Due to this integration, ICEs are maintained stably, even in the absence of selective pressure. As a result, the frequency of antibiotic resistance in Bacteroides has increased dramatically over the last 30 years (2). ICEs also carry genes to regulate and carry out their own transfer. Furthermore, some ICEs can mobilize coresident genetic elements that could not otherwise transfer (3). Because of their benefit to the bacterial host and their ability to transfer among organisms, ICEs are widespread in both Gram-positive and -negative bacterial populations (2).CTnDOT is a well-characterized ICE found in Bacteroides species. It carries the ermF and tetQ genes that encode resistance to erythromycin and tetracycline, respectively. Exposure to tetracycline induces the excision and transfer of CTnDOT. CTnDOT integration and excision require an integrase, IntDOT, and a hostencoded protein factor. IntDOT is a tyrosine recombinase and is in the same family of enzymes as Int, Flp, XerC, XerD, and Cre (4). IntDOT contains five of the six conserved amino acid residues that form the catalytic sites of tyrosine recombinases (5, 6). These enzymes perform strand exchanges by a site-...

show abstract

Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA

Cited by 6 publications

References 44 publications

The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems

The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems

Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

The Bacteroides thetaiotaomicron Protein Bacteroides Host Factor A Participates in Integration of the Integrative Conjugative Element CTnDOT into the Chromosome

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