Intercellular transfer of miR-126-3p by endothelial microparticles reduces vascular smooth muscle cell proliferation and limits neointima formation by inhibiting LRP6

Jansen, Felix; Stumpf, Tobias; Proebsting, Sebastian; Franklin, Bernardo S.; Wenzel, Daniela; Pfeifer, Philipp; Flender, Anna; Schmitz, Theresa; Yang, Xiaoyan; Fleischmann, Bernd K.; Nickenig, Georg; Werner, Nikos

doi:10.1016/j.yjmcc.2016.12.005

Cited by 114 publications

(88 citation statements)

References 42 publications

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“…Vascular SMC proliferation and migration from the medial space is often an early response to pathological cues. Patients with coronary artery disease exhibit an inverse relationship between percutaneous coronary intervention rate and the expression of microRNA-126 in circulating EVs [8]. Intravenous injection of endothelial-derived EVs loaded with microRNA-126 prevents SMC proliferation, migration, and neointima formation in a mouse model of vascular injury.…”

Section: Extracellular Vesicles As Mediators Of Intracellular Communimentioning

confidence: 99%

Extracellular vesicles in cardiovascular homeostasis and disease

Hutcheson

Aikawa

2018

Current Opinion in Cardiology

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Purpose of review Extracellular vesicles (EVs) have emerged as one of the most important means through which cells interact with each other and the extracellular environment, but EV research remains challenging due to their small size and limited amount of material required for traditional molecular biology assays. The advent of new technologies and standards in the field, however, have led to increased mechanistic insight into EV function. Herein, the latest studies on the role of EVs in cardiovascular physiology and pathology are discussed. Recent findings EVs help control cardiovascular homeostasis and remodeling by mediating communication between cells and directing alterations in the extracellular matrix to respond to changes in the environment. The message carried from the parent cell to extracellular space can be intended for both local (within the same tissue) and distal (downstream of blood flow) targets. Pathological cargo loaded within EVs could further result in various diseases. On the other hand, new studies indicate that injection of EVs obtained from cultured cells into diseased tissues can promote restoration of normal tissue function. Summary EVs are an integral part of cell and tissue function, and harnessing the properties inherent to EVs may provide a therapeutic strategy to promote tissue regeneration.

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Section: Extracellular Vesicles As Mediators Of Intracellular Communimentioning

confidence: 99%

Extracellular vesicles in cardiovascular homeostasis and disease

Hutcheson

Aikawa

2018

Current Opinion in Cardiology

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“…From the results of cell culture on the bilayered electrospun membranes (Fig. ), it can be indicated that miR‐145 hardly influenced the physiological characteristics of VECs, and miR‐126 introduction reduced the VSMC proliferation rate, which could be explained by inhibiting LRP6 . However, miR‐126 and miR‐145 presented no obvious synergy effect in this study.…”

Section: Discussionmentioning

confidence: 54%

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Cui

Wen

Zhou

et al. 2018

J Biomedical Materials Res

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Clinical utility of small‐diameter vascular grafts is still challenging in blood vessel regeneration owing to thrombosis and intimal hyperplasia. To cope with the issues, modulation of gene expression via microRNAs (miRNAs) could be a feasible approach by rational regulating physiological activities of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). Our previous studies demonstrated that individually loaded miRNA‐126 (miR‐126) or miRNA‐145 (miR‐145) in the electrospun membranes showed the tendency to promote vascular regeneration. In this work, the bilayered electrospun graft in 1.5‐mm diameter was developed by emulsion electrospinning to dual‐load miR‐126 and miR‐145 for target regulation of both VECs and VSMCs, respectively. Accelerated release of miR‐126 was achieved by introducing poly(ethylene glycol) in the inner electrospun poly(ethylene glycol)‐b‐poly(l‐lactide‐co‐caprolactone) ultrafine fibrous membrane, reaching 61.3 ± 1.2% of the cumulative release in the initial 10 days, whereas the outer electrospun poly(l‐lactide‐co‐glycolide) membrane composed of microfibers fulfilled prolonged release of miR‐145 for about 56 days. In vivo tests suggested that dual‐loading with miR‐126 and miR‐145 in the bilayered electrospun membranes could modulate both VECs and VSMCs for rapid endothelialization and hyperplasia inhibition as well. It is reasonably expected that dual target‐delivery of miR‐126 and miR‐145 in the electrospun vascular grafts has effective potential for small‐diameter vascular regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 371–382, 2019.

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“…The human umbilical vein EC (HUVEC, Lonza, UK) were cultivated and maintained in EndoGRO‐VEGF medium (SCME002; Merck‐Millipore, Billerica, MA) and 1% penicillin–streptomycin (Cellgro) under standard cell culture condition in an incubator at 37°C, 5% CO 2 humidified atmosphere and 95% air (Jansen et al, ). The cell passages of three to five were used at a confluency of 70–80% (Jansen et al, ). The HUVEC were rinsed with PBS and detached using Tryple E Express (Gibco).…”

Section: Methodsmentioning

confidence: 99%

Effects of different polyaniline emeraldine compositions in electrodepositing ginsenoside encapsulated poly(lactic‐co‐glycolic acid) microcapsules coating: Physicochemical characterization and in vitro evaluation

Lukman

Saidin

2020

J Biomedical Materials Res

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Even though drug‐eluting stent (DES) has prominently reduced restenosis, however, its complication of delayed endothelialization has caused chronic side effect. A coating of ginseng‐based biodegradable polymer could address this issue due to its specific therapeutic values. However, deposition of this type of stable coating on metallic implant often scarce. Therefore, in this study, different polyaniline (PANI) emeraldine compositions were adopted to electrodeposit ginsenoside encapsulated poly(lactic‐co‐glycolic acid) microcapsules coating. The coating surfaces were analyzed using attenuated total reflectance‐Fourier transform infrared spectroscopy, X‐ray photoelectron spectroscopy, scanning electron microscopy, contact angle, and atomic force microscopy instruments. A month coating stability was then investigated with an evaluation of in vitro human umbilical vein endothelial cell analyses consisted of cytotoxicity and cells attachment assessments. The 1.5 mg PANI emeraldine has assisted the formation of stable, uniform, and rounded microcapsules coating with appropriate wettability and roughness. Less than 1.5 mg PANI emeraldine was not enough to drive the formation of microcapsules coating while greater than 1.5 mg caused the deposition of melted microcapsules. The similar coating also has promoted greater cells proliferation and attachment compared to other coating variation. Therefore, the utilization of electrodeposition to deposit a drug‐based polymer coating could be implemented to develop DES, in accordance to stent implantation which ultimately aims for enrich endothelialization.

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Intercellular transfer of miR-126-3p by endothelial microparticles reduces vascular smooth muscle cell proliferation and limits neointima formation by inhibiting LRP6

Cited by 114 publications

References 42 publications

Extracellular vesicles in cardiovascular homeostasis and disease

Extracellular vesicles in cardiovascular homeostasis and disease

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Effects of different polyaniline emeraldine compositions in electrodepositing ginsenoside encapsulated poly(lactic‐co‐glycolic acid) microcapsules coating: Physicochemical characterization and in vitro evaluation

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