Interesterification of milkfat with soybean oil catalysed by Rhizopus oryzae lipase immobilised on SiO₂‐PVA on packed bed reactor

Abstract

The potential of the lipase from Rhizopus oryzae immobilised on SiO 2 -PVA to catalyse the interesterification of the milkfat with soybean oil in a packed bed reactor running on continuous mode was evaluated. The reactor operated continuously for 35 days at 45°C, and during 12 days, no significant decrease in the initial lipase activity was verified. Interesterification yields were in the range from 35 to 38%wt, which gave an interesterified product having 59% lower consistency in relation to non-interesterified blend. Results showed the potential of the lipase from Rhizopus oryzae to mediate the interesterification of milkfat with soybean oil in packed bed reactor, attaining a more spreadable product under a cool temperature. The biocatalyst operational stability was assessed and an inactivation profile was found to follow the Arrhenius model, revealing values of 34 days and 0.034 day )1 , for half-life and a deactivation coefficient, respectively.

Continuous enzymatic interesterification of milkfat with soybean oil produces a highly spreadable product rich in polyunsaturated fatty acids

“…A parameter of fundamental importance, when working with processes that involve immobilized enzymes, is the operational stability of the biocatalyst , that can be measured by the half‐life time.…”

“…The high operational stability of this non‐commercial immobilized preparation is rather promising for the industrial application of lipase‐catalyzed interesterification in a fluidized‐bed reactor. These results may be attributed to the immobilizing procedure applied to this lipase that is a method developed by our research group . The carrier silica‐polyvinyl alcohol (SiO 2 ‐ PVA) was prepared by the sol–gel technique, using a simple but effective procedure which gave an immobilized lipase preparation with good activity and stability that has been successfully used in different processes .…”

Performance of packed bed reactor on the enzymatic interesterification of milk fat with soybean oil to yield structure lipids

“…The recovered immobilised lipase was then washed with tert-butanol to remove any substrate or product retained in the matrix. Hydrolytic activity was determined by the olive oil emulsion method according to the methodology described by Paula, Nunes, Santos, and De Castro (2011). The inactivation constant (kd) and half-life (t 1/2 ) for the immobilised lipase were calculated as described by Costa-Silva, Teixeira, Carvalho, Mendes, and De Castro (2014).…”

Reestruturação da gordura de leite por interesterificação enzimática empregando lipase imobilizada: otimização das condições reacionais e operacionais

“…Deste modo, pode-se observara a dessorção do biocatalisador, uma vez que sua ocorrência está relacionada às alterações conformacionais da proteína e às condições hidrodinâmicas do sistema reacional FERNANDES, 2003). Em estudo recente (PAULA et al, 2011) FOGLER, 2009).…”