Performance of packed bed reactor on the enzymatic interesterification of milk fat with soybean oil to yield structure lipids

Abstract

a b s t r a c tThe interesterification reaction of milk fat with soybean oil mediated by Rhizopus oryzae lipase immobilised on a hybrid organic-inorganic support of polysiloxane-polyvinyl alcohol (SiO 2 -PVA) was assessed in a packed bed reactor running in continuous flow (45 C, blend of milk fat and soybean oil 65:35%, w/ w). The reactor performance was quantified for different flow rates corresponding to space times (0.5 and 4 h). For each condition, the influence of the space time on the consistency, free fatty acid and solid fat content (SFC, %) was determined. Percentages of reduction on consistency (71.2%) were obtained in relation to the initial blend (1431 ± 42 gf cm À2 ) and the SFC values (SFC 10 C ¼ 39.6%; SFC 20 C ¼ 20.5% and SFC 35 C ¼ 0.2%) were within the ranges considered ideal for spreads with satisfactory spreadability. The immobilised lipase was stable with respect to its catalytic characteristics, exhibiting a half-life of 39 days.

Enzymatic synthesis of structured lipids from grape seed (Vitis vinifera L.) oil in associated packed bed reactors

“…Residence time distribution is very important to study fluid velocity patterns and determine the best flow rate for continuous reactors [27,41,50]. A research conducted at the same laboratory of the present study [50] evaluated the performance of a packed bed reactor in the enzymatic interesterification of milk fat with soybean oil for the production of structured lipids and observed a difference of less than 20% between theoretical and experimental results.…”

Produção de sucedâneo da gordura do leite humano por interesterificação enzimática: uma revisão

“…Muitos trabalhos que não apresentaram os dois termos principais buscados, também servem como base de referência para o estudo da produção de HFMS, pois fazem a avaliação das variáveis que interferem no processo de interesterificação Research, Society andDevelopment, v. 10, n. 3, e36210313469, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i3.13469 enzimática, como monitoramento do reator e teste de estabilidade dos produtos e de eficiência das enzimas por exemplo (De Paula et al, 2018;Cai et al, 2015;Kotani et al, 2015;Zhao et al, 2019;.…”

“…De Paula et al, (2018) por exemplo, avaliaram a eficiência do reator de leito fixo (PBR) através da interesterificação de gordura do leite com óleo de soja, em meios isentos de solventes, catalisada por uma preparação de lipase a partir de R. oryzae imobilizado em um suporte híbrido orgânico-inorgânico de álcool polisiloxano-polivinílico (SiO -PVA) funcionando em modo contínuo. O uso de biocatalisadores em processos em escala laboratorial tem sido realizado em diferentes configurações de reatores, mas o processo contínuo é mais vantajoso em comparação com o lote.…”

Synthesis, physicochemical properties, and health aspects of structured lipids: A review

“…Second, it is also important to select suitable reactors. Compared to stirring batch reactors which are mostly used in scientific researches, continuous fixed bed reactors are more common in an industrial setting for large‐scale production of SLs with a superior efficacy (De Paula, Nunes, De Castro, & Dos Santos, ; Kim & Akoh, ; Wang et al., ). Xiao et al.…”

“…Second, it is also important to select suitable reactors. Compared to stirring batch reactors which are mostly used in scientific researches, continuous fixed bed reactors are more common in an industrial setting for large-scale production of SLs with a superior efficacy (De Paula, Nunes, De Castro, & Dos Santos, 2018;Kim & Akoh, 2006;Wang et al, 2016 showing excellent reusability. Xie and Zang (2017) F I G U R E 2 (a) Illustration of the construction of Fe 3 O 4 @SiO 2 @{CHI/HA} 3 -based on layer-by-layer self-assembly technique and its application for the immobilization of lipase and synthesis of OPO (Cai et al, 2019); (b) Illustration of a continuous flow bioreactor based on immobilized lipase using cellulose acetate monoliths as the support for production of phytosterol esters (Xiao et al, 2018) lipase using a hierarchically porous cellulose monolith as the support, based on which a monolithic continuous flow bioreactor (MCFB) was constructed to produce phytosterol esters from phytosterol and oleic acid ( Figure 2b).…”