Interference with <i>Aggregatibacter actinomycetemcomitans</i>: colonization of epithelial cells under hydrodynamic conditions

Sliepen, I.; Essche, Mark Van; Loozen, Gitte; Eldere, Johan Van; Quirynen, Marc; Teughels, Wim

doi:10.1111/j.1399-302x.2009.00531.x

Cited by 23 publications

(20 citation statements)

References 31 publications

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“…S. mitis has been described as a bacterium with an ecological control function in the oral cavity. Precisely, S. mitis could inhibit A. actinomycetemcomitans colonization [41]–[45]. Our results support this observation and associate the inhibitory effects of S. mitis to both the initial step as well as the ensuing multiplication ability of A. actinomycetemcomitans .…”

Section: Discussionsupporting

confidence: 85%

Setup of an In Vitro Test System for Basic Studies on Biofilm Behavior of Mixed-Species Cultures with Dental and Periodontal Pathogens

et al. 2010

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BackgroundCaries and periodontitis are important human diseases associated with formation of multi-species biofilms. The involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. This study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. The setup allows a rapid screening for effects in the mutual species interaction. Furthermore, it is easy to handle, inexpensive, and reproducible.Methods Streptococcus mitis, S. salivarius and S. sanguinis, typical inhabitants of the healthy oral cavity, S. mutans as main carriogenic species, and Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra, S. intermedius and Aggregatibacter actinomycetemcomitans as periodontitis-associated bacteria, were investigated for their biofilm forming ability. Different liquid growth media were evaluated. Safranin-staining allowed monitoring of biofilm formation under the chosen conditions. Viable counts and microscopy permitted investigation of biofilm behavior in mixed-species and transwell setups.Findings S. mitis, F. nucleatum, P. gingivalis and P. micra failed to form biofilm structures. S. mutans, S. sanguinis, S. intermedius and S. salivarius established abundant biofilm masses in CDM/sucrose. A. actinomycetemcomitans formed patchy monolayers. For in depth analysis S. mitis, S. mutans and A. actinomycetemcomitans were chosen, because i) they are representatives of the physiological-, cariogenic and periodontitis-associated bacterial flora, respectively and ii) their difference in their biofilm forming ability. Microscopic analysis confirmed the results of safranin staining. Investigation of two species combinations of S. mitis with either S. mutans or A. actinomycetemcomitans revealed bacterial interactions influencing biofilm mass, biofilm structure and cell viability.ConclusionsThis setup shows safranin staining, microscopic analysis and viable counts together are crucial for basic examination and evaluation of biofilms. Our experiment generated meaningful results, exemplified by the noted S. mitis influence, and allows a fast decision about the most important bacterial interactions which should be investigated in depth.

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Section: Discussionsupporting

confidence: 85%

Setup of an In Vitro Test System for Basic Studies on Biofilm Behavior of Mixed-Species Cultures with Dental and Periodontal Pathogens

et al. 2010

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“…Using different cell lines (gingival, bronchial, pharyngeal, and intestinal epithelial mucosa), it has been shown that NF-B activation and IL-8 production were reduced after stimulation with flagellin from pathogens such as Yersinia enterolitica, Aggregatibacter actinomycetemcomitans, Pseudomonas aeruginosa, and Salmonella enterica subsp. enterica serovar Typhimurium (20,25,27,28). Although here we described anti-inflammatory properties of S. salivarius on stimulated IECs, we also showed that the immunomodulation properties of these strains were different on immunocompetent PBMCs.…”

Section: Discussionmentioning

confidence: 53%

“…The S. salivarius TOVE-R strain has been reported to be a successful antagonist of virulent streptococci involved in tooth decay or pharyngitis, such as Streptococcus mutans, Streptococcus sobrinus, and Streptococcus pyogenes (19), or pathogens involved in periodontitis (20,21). The inhibitory activity toward S. pyogenes and Streptococcus pneumoniae has been attributed to bacteriocin production (22)(23)(24).…”

mentioning

confidence: 99%

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Kaci

Goudercourt

Dennin

et al. 2014

Appl Environ Microbiol

161

122

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f Streptococcus salivarius is one of the first colonizers of the human oral cavity and gut after birth and therefore may contribute to the establishment of immune homeostasis and regulation of host inflammatory responses. The anti-inflammatory potential of S. salivarius was first evaluated in vitro on human intestinal epithelial cells and human peripheral blood mononuclear cells. We show that live S. salivarius strains inhibited in vitro the activation of the NF-B pathway on intestinal epithelial cells. We also demonstrate that the live S. salivarius JIM8772 strain significantly inhibited inflammation in severe and moderate colitis mouse models. These in vitro and in vivo anti-inflammatory properties were not found with heat-killed S. salivarius, suggesting a protective response exclusively with metabolically active bacteria.

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“…sanguinis may decrease the release of IL-8 by the epithelial cells stimulated with Fusobacterium nucleatum and A. actinomycetemcomitans [62][63][64].…”

Section: Probiotic Effect On Immune Responsesmentioning

confidence: 99%

Probiotics and Periodontal Diseases

Morales¹,

Bravo²,

Bedoya³

et al. 2017

Insights Into Various Aspects of Oral Health

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Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Probiotics have been used to directly modify the resident oral microbiome and proposed to modulate immune responses. In dentistry, probiotics have been employed as useful adjuncts for the reduction of caries development, suppressing oral Candida infection and controlling halitosis. Plaque-induced gingivitis is a gingival inflammation caused by the adherent bacterial biofilm around teeth. Gingivitis and periodontitis are considered to be a continuum of the same inflammatory process, although many gingivitis lesions do not progress to periodontitis. Periodontitis is an inflammatory process that affects the attachment structures of teeth. It constitutes a second cause of tooth loss worldwide. Conventional treatment modalities of periodontal disease include non-surgical and/or surgical management, with an emphasis on mechanical debridement. However, mechanical debridement as a sole therapy is not always effective to improve clinical parameters. A growing number of studies support probiotic therapy to prevent or treat gingivitis and periodontitis. Oral administration of probiotics is an effective adjunct in reducing pathogenic bacteria and improving clinical signs of disease. Probiotics may serve as adjunct or replacement therapy substitute antibiotics in managing human periodontal infections in future.

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Interference with Aggregatibacter actinomycetemcomitans: colonization of epithelial cells under hydrodynamic conditions

Abstract: These data confirm the possibility of applying beneficial bacteria in periodontal treatment.

Cited by 23 publications

References 31 publications

Setup of an In Vitro Test System for Basic Studies on Biofilm Behavior of Mixed-Species Cultures with Dental and Periodontal Pathogens

Setup of an In Vitro Test System for Basic Studies on Biofilm Behavior of Mixed-Species Cultures with Dental and Periodontal Pathogens

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Probiotics and Periodontal Diseases

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