In this article, a comprehensive uncertainty analysis will be given of a popular 2D particle measuring technique called Interferometric Laser Imaging for Droplet Sizing (ILIDS). This includes the first estimates on the influence of droplet non-sphericity. Next to this, the uncertainty on the frequency determination and the calibration procedures were also investigated. To this end, the two most common calibration procedures were experimentally compared. This showed that it often dominates the total uncertainty. Design guidelines are then derived concerning observation angle, out-focus disk size and calibration procedure which could lead to a combined uncertainty below 1.5%.