Interfertile oaks in an island environment: I. High nuclear genetic differentiation and high degree of chloroplast DNA sharing between Q. alnifolia and Q. coccifera in Cyprus. A multipopulation study

Neophytou, Charalambos; Dounavi, Aikaterini; Fink, Siegfried; Aravanopoulos, Filippos A.

doi:10.1007/s10342-010-0442-8

Cited by 23 publications

(40 citation statements)

References 60 publications

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“…pyrenaica, this study) and between two species from the section Ilex (Q. alnifolia-Q. coccifera; Neophytou et al, 2011). GOT021, on the other hand, has been detected as an outlier in the section Quercus (this study) and in the North American oaks, section Lobatae (Q. ellipsoidalis-Q.…”

Section: Confounding Effects On Neutrality Testingmentioning

confidence: 99%

A linkage disequilibrium perspective on the genetic mosaic of speciation in two hybridizing Mediterranean white oaks

et al. 2014

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We analyzed the genetic mosaic of speciation in two hybridizing Mediterranean white oaks from the Iberian Peninsula (Quercus faginea Lamb. and Quercus pyrenaica Willd.). The two species show ecological divergence in flowering phenology, leaf morphology and composition, and in their basic or acidic soil preferences. Ninety expressed sequence tag-simple sequence repeats (EST-SSRs) and eight nuclear SSRs were genotyped in 96 trees from each species. Genotyping was designed in two steps. First, we used 69 markers evenly distributed over the 12 linkage groups (LGs) of the oak linkage map to confirm the species genetic identity of the sampled genotypes, and searched for differentiation outliers. Then, we genotyped 29 additional markers from the chromosome bins containing the outliers and repeated the multilocus scans. We found one or two additional outliers within four saturated bins, thus confirming that outliers are organized into clusters. Linkage disequilibrium (LD) was extensive; even for loosely linked and for independent markers. Consequently, score tests for association between two-marker haplotypes and the 'species trait' showed a broad genomic divergence, although substantial variation across the genome and within LGs was also observed. We discuss the influence of several confounding effects on neutrality tests and review the evolutionary processes leading to extensive LD. Finally, we examine how LD analyses within regions that contain outlier clusters and quantitative trait loci can help to identify regions of divergence and/or genomic hitchhiking in the light of predictions from ecological speciation theory.

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Section: Confounding Effects On Neutrality Testingmentioning

confidence: 99%

A linkage disequilibrium perspective on the genetic mosaic of speciation in two hybridizing Mediterranean white oaks

et al. 2014

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“…Despite of a high frequency of interspecific gene flow that has been inferred from many combinations in oaks, most of the studies have been carried out in mixed stands consisting of only two parental taxa and their hybrids (e.g., Tovar-Sánchez and Oyama 2004;Valbuena-Carabana et al 2005;Albarrán-Lara et al 2010;Neophytou et al 2011;Lagache et al 2013). Nevertheless, other species complexes of simultaneous hybridization among more parental taxa have been studied in nature (Dodd and Afzal-Rafii 2004;Curtu et al 2007;Lepais et al 2009;Peñaloza-Ramírez et al 2010;Moran et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…However, the wide intraspecific variability of leaf and acorn morphology within the genera Quercus commonly limits its utility for hybridization diagnosis (Curtu et al 2007). In recent studies, valuable information to detect and evaluate the level of hybridization and introgression direction has been obtained using genetic markers such as microsatellites, a complementary tool to study hybridization and introgression in oaks (e.g., Muir et al, 2000;Valbuena-Carabana et al 2005;Curtu et al 2007;Burgarella et al 2009;Lepais et al 2009;Peñaloza-Ramírez et al 2010;Neophytou et al 2011;Lagache et al 2013). In general, hybridization has been intensively studied in white oaks (section Quercus); however, few studies have examined gene flow in red oaks (section Lobatae, Moran et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Hybridization of Quercus castanea (Fagaceae) across a red oak species gradient in Mexico

et al. 2014

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Interspecific gene flow between more than two species is a common phenomenon in oaks, which can occur simultaneously among different species, promoting the transfer of genetic material across species boundaries. However, the hybridization dynamics in multispecies hybrid zones remain unknown. In this study, we provide genetic evidence of hybridization and introgression of Quercus castanea across a natural gradient of red oak species richness. We analyzed five populations recognized morphologically as ''pure'' Q. castanea, one allopatric and four sympatric populations, where the number of red oak species associated with Q. castanea ranged from one to four. Also, one allopatric population of each red oak species that occurs in sympatry with Q. castanea was chosen as reference population (Q. crassipes, Q. laurina, Q. mexicana and Q. crassifolia). In total, six nSSRs were used in 10 and 20 individuals from each allopatric and sympatric populations, respectively. Our results showed that allopatric populations formed completely distinct genetic clusters.In sympatric populations, we found evidence of hybridization and introgression among Q. castanea and three of its associated red oak species. However, the occurrence and frequency of hybrids between Q. castanea and these species varied among stands. Our analyses provide evidence and new insights into hybridization and introgression dynamics within a Mexican red oak species complex, through a focal species, Q. castanea.

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“…Chlorotype nomenclature is introduced in the companion paper of Neophytou et al (2010a) and is followed here as well. Haplotypic diversity was calculated using the software Rarefac (Petit et al 1997b).…”

Section: Analysis Of Chloroplast Dna Diversity and Differentiationmentioning

confidence: 99%

Interfertile oaks in an island environment. II. Limited hybridization between Quercus alnifolia Poech and Q. coccifera L. in a mixed stand

et al. 2011

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Hybridization and introgression betweenQuercus alnifolia Poech and Q. coccifera L. is studied by analyzing morphological traits, nuclear and chloroplast DNA markers. The study site is a mixed stand on Troodos Mountains (Cyprus), and the analyzed material includes both adult trees and progenies of specific mother trees. Multivariate analysis of morphological traits shows that the two species can be well distinguished using simple leaf morphometric parameters. A lower genetic diversity in Q. alnifolia than in Q. coccifera and a high interspecific differentiation between the two species are supported by an analysis of nuclear and chloroplast microsatellites. The intermediacy of the four designated hybrids is verified by both leaf morphometric and genetic data. Analysis of progeny arrays provides evidence that interspecific crossings are rare. This finding is further supported by limited introgression of chloroplast genomes. Reproductive barriers (e.g. asynchronous phenology, postzygotic incompatibilities) might account for this result. A directionality of interspecific gene flow is indicated by a genetic assignment analysis of effective pollen clouds with Q. alnifolia acting as pollen donor. Differences in flowering phenology and species distribution in the stand may have influenced the direction of gene flow and the genetic differentiation among effective pollen clouds of different mother trees within species.

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Interfertile oaks in an island environment: I. High nuclear genetic differentiation and high degree of chloroplast DNA sharing between Q. alnifolia and Q. coccifera in Cyprus. A multipopulation study

Cited by 23 publications

References 60 publications

A linkage disequilibrium perspective on the genetic mosaic of speciation in two hybridizing Mediterranean white oaks

A linkage disequilibrium perspective on the genetic mosaic of speciation in two hybridizing Mediterranean white oaks

Hybridization of Quercus castanea (Fagaceae) across a red oak species gradient in Mexico

Interfertile oaks in an island environment. II. Limited hybridization between Quercus alnifolia Poech and Q. coccifera L. in a mixed stand

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