Interleukin-17A as a Biomarker for Bovine Tuberculosis

Waters, W. Ray; Maggioli, Mayara F.; Palmer, Mitchell V.; Thacker, Tyler C.; McGill, Jodi L.; Vordermeier, H. Martin; Berney-Meyer, Linda; Jacobs, William R.; Larsen, Michelle H.

doi:10.1128/cvi.00637-15

Cited by 44 publications

(41 citation statements)

References 59 publications

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“…There have been several publications reporting the expression of mRNA encoding bovine IL-17A (and other IL-17 family members) as measured by RT-PCR [9, 39, 40]. There have also been publications reporting the quantification of IL-17A protein in cattle using the anti-bovIL-17A ELISA Vetset marketed by Kingfisher Biotech [10, 11, 39, 41, 42].…”

Section: Discussionmentioning

confidence: 99%

Enhancing the toolbox to study IL-17A in cattle and sheep

Wattegedera

Corripio-Miyar

Pang

et al. 2017

Vet Res

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The development of methods to detect cytokine expression by T cell subsets in ruminants is fundamental to strategic development of new livestock vaccines for prevention of infectious diseases. It has been possible to detect T cell expression of IFN-γ, IL-4 and IL-10 in ruminants for many years but methods to detect expression of IL-17A are relatively limited. To address this gap in capability we have cloned bovine and ovine IL-17A cDNAs and expressed biologically-active recombinant proteins in Chinese Hamster Ovary (CHO) cells. We used the transfected CHO cells to screen commercially-available antibodies for their ability to detect IL-17A expression intracellularly and in culture supernates. We demonstrate that an ELISA for bovine IL-17A detects native ovine IL-17A. Moreover, the constituent polyclonal antibodies (pabs) in the ELISA were used to enumerate peripheral blood mononuclear cells (PBMC) expressing IL-17A from cattle and sheep by ELISpot. We identified two monoclonal antibodies (mabs) that detect recombinant intracellular IL-17A in CHO cells by flow cytometry. One of these mabs was used to detect native intracellular IL-17A expression in PBMC in conjunction with cell surface phenotyping mabs [CD4+ve, CD8+ve and Workshop Cluster 1 (WC-1)+ve gamma-delta (γδ)] we show that distinct T cell subsets in cattle (defined as CD4+ve, CD8+ve or WC-1+ve) and sheep (defined as CD4+ve or WC-1+ve) can express IL-17A following activation. These novel techniques provide a solid basis to investigate IL-17A expression and define specific CD4+ve T cell subset activation in ruminants.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0426-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Enhancing the toolbox to study IL-17A in cattle and sheep

Wattegedera

Corripio-Miyar

Pang

et al. 2017

Vet Res

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“…Genome-wide gene expression profiling has been increasingly used to investigate pathogenetic mechanism and identify potential biomarkers for various human diseases (20,21). RNA sequencing (RNA-seq) is a widely used method to study overall transcriptional activity and has a broad coverage.…”

Section: Introductionmentioning

confidence: 99%

RNA‑seq analysis of blood of valproic acid‑responsive and non‑responsive pediatric patients with epilepsy

Wang

2019

Exp Ther Med

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Epilepsy is the most common chronic neurological disorder, affecting ~70 million individuals worldwide. However, approximately one-third of the patients are refractory to epilepsy medication. Of note, 100% of patients with genetic epilepsy who are resistant to the traditional drug, valproic acid (VPA), are also refractory to the other anti-epileptic drugs. The aim of the present study was to compare the transcriptomes in VPA responders and non-responders, to explore the mechanism of action of VPA and identify possible biomarkers to predict VPA resistance. Thus, RNA-seq was employed for transcriptomic analysis, differentially expressed genes (DEGs) were analyzed using Cuffdiff software and the DAVID database was used to infer the functions of the DEGs. A protein-protein interaction network was obtained using STRING and visualized with Cytoscape. A total of 389 DEGs between VPA-responsive and non-responsive pediatric patients were identified. Of these genes, 227 were upregulated and 162 were downregulated. The upregulated DEGs were largely associated with cytokines, chemokines and chemokine receptor-binding factors, whereas the downregulated DEGs were associated with cation channels, iron ion binding proteins, and immunoglobulin E receptors. In the pathway analysis, the toll-like receptor signaling pathway, pathways in cancer, and cytokine-cytokine receptor interaction were mostly enriched by the DEGs. Furthermore, three modules were identified by protein-protein interaction analysis, and the potential hub genes, chemokine (C-C motif) ligand 3 and 4, chemokine (C-X-C motif) ligand 9, tumor necrosis factor-α and interleukin-1β, which are known to be closely associated with epilepsy, were identified. These specific chemokines may participate in processes associated with VPA resistance and may be potential biomarkers for monitoring the efficacy of VPA.

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“…Several Th17-associated cytokine genes including IL-17A, IL-17F, IL-22, etc. have been shown to be up-regulated (in response to BPPD) in M. bovis-infected cattle [74]. Moreover, IFN-γ and IL-17A production are interlinked thus suggesting the utility of Th17-associated cytokines as potential biomarkers of protection in bovine TB.…”

Section: Immunological Correlates Of Protection Studies In Cattlementioning

confidence: 96%

“…Indeed, enhanced IL-22 and IL-17A mRNA expression in M. bovis-infected cattle following elicitation with BPPD has also been reported [82] thus proposing that evaluation of these cytokines could be useful biomarkers in bovine infection. To investigate the precise cell populations involved in production of these cytokines, Steinbach et al [83] [74,79]. These observations might be useful for further understanding the immunopathology of bovine TB and to produce more accurate immunodiagnostic reagents.…”

Section: Immunological Correlates Of Protection Studies In Cattlementioning

confidence: 99%

Cattle as Experimental Model to Study Immunopathogenesis of Tuberculosis

Mehta¹,

Dharra²,

Thakur³

2017

Mycobact Dis

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Various animal models are used to study the immunology, genetics and molecular biology of tuberculosis (TB) as well as for testing the new vaccines and drugs. Mice are widely used to study the immunology of chronic TB infection, while guinea pigs are used for aerosol TB infection and rabbits are used to study the lung cavitations. Cattle are natural host to Mycobacterium bovis infection, which act as a connecting link between the small laboratory animals and human counterparts for testing the vaccine efficacy. By using cattle as an experimental model, the disease outcome is understood through natural infection with M. bovis and a comparison can be made with M. tuberculosis infection. In this manuscript, the utility of cattle in understanding the progression of disease and the immunological correlates to evaluate the protective efficacy of vaccines are described.

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Interleukin-17A as a Biomarker for Bovine Tuberculosis

Cited by 44 publications

References 59 publications

Enhancing the toolbox to study IL-17A in cattle and sheep

Enhancing the toolbox to study IL-17A in cattle and sheep

RNA‑seq analysis of blood of valproic acid‑responsive and non‑responsive pediatric patients with epilepsy

Cattle as Experimental Model to Study Immunopathogenesis of Tuberculosis

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