Interleukin-8 expression by mammary gland endothelial and epithelial cells following experimental mastitis infection with E. coli

McClenahan, David; Krueger, Rebecca; Lee, Haa-Yung; Thomas, Chester B.; Kehrli, Marcus E.; Czuprynski, Charles J.

doi:10.1016/j.cimid.2006.03.001

Cited by 23 publications

(12 citation statements)

References 36 publications

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“…Possibly, in these sows there may be abundant and regulated release of anti-inflammatory mediators [40,53], limiting the production of the inflammatory cytokines. However, in accordance with our results, previous studies in cows have shown a significant increase in the proinflammatory cytokines at the level of both mRNA [33,37] and protein [6,11,26,32,45,48,54] in either mammary tissues or milk collected from the infected glands following intramammary inoculation with E. coli. The results here indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.…”

Section: Discussionsupporting

confidence: 93%

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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-Twelve healthy primiparous sows received intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period preceding parturition. Mammary gland biopsy samples were taken immediately before inoculation (0 h) and from the inoculated and the contralateral noninoculated glands 24 h after inoculation. The analyses of interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) by immunohistochemistry revealed that the production of these proinflammatory cytokines significantly increased in the inoculated mammary glands of sows that developed clinical signs of mastitis (affected group, n = 4) 24 h after inoculation. This was also true for IL-8 in the inoculated mammary glands of sows that did not develop clinical signs of mastitis (nonaffected group, n = 8). Sows that developed clinical signs of mastitis displayed significantly lower constitutive production of IL-1β than did sows that remained clinically healthy. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.cytokine / immunohistochemistry / mastitis / pig / E. coli

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Section: Discussionsupporting

confidence: 93%

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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“…Following in vitro culture with LPS or E. coli , several cytokines and chemokines are produced by bovine mammary epithelial cells, as shown at the mRNA and protein level (Boudjellab et al ., 1998; Okada et al ., 1999; Wellnitz and Kerr, 2004; Pareek et al ., 2005; Strandberg et al ., 2005; Lahouassa et al ., 2007). In mammary epithelial cells from cows experimentally infected with E. coli , these inflammatory proteins are increased (McClenahan et al ., 2006) and secreted in milk as well (Riollet et al ., 2000). In addition, NF‐κB activation seemed to be essential for the production of bactericidal peptides following in vitro culture of bovine and murine mammary epithelial cells with E. coli (Yang et al ., 2006).…”

Section: Discussionmentioning

confidence: 99%

In vivo imaging of NF-κB activity during Escherichia coli-induced mammary gland infection

et al. 2008

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SummaryIn mammary gland infections, the contribution of NF-kB is not well defined, and was therefore investigated following intramammary inoculation of Escherichia coli. Non-invasive real-time in vivo imaging of the transcription factor activation was performed in mammary glands of transgenic mice expressing luciferase under the control of NF-kB. Bacterial inoculation resulted in a major increase in luminescence as compared with control glands. This activation was confirmed by immunohistochemical nuclear staining of the NF-kB p65 subunit in mammary epithelium of infected glands, while nuclear p50 was not detected. The systemic response to the intramammary inoculation of Escherichia coli was also studied. NF-kB activation in the liver increased over time, and a relatively mild but longer-lasting response was observed as compared with the acute hepatic response of mice receiving lipopolysaccharide. This systemic reaction was confirmed by increased circulating levels of the acute phase protein serum amyloid A, tumour necrosis factor-a and interleukin-6. In addition, high concentrations of both cytokines in the mammary gland inoculated with bacteria showed that the infection was also well established at the local level. These results indicate that in vivo monitoring of NF-kB activation is an attractive novel approach to study mammary gland inflammation, and that this transcription factor is imperative in the early stages of the host immune response towards coliform intramammary infections, both at the local and systemic level.

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“…Both epithelial and endothelial cells contribute to the production of IL-8 during Escherichia coli infection. In cows experimentally infected with E. coli via injection in the teat canal, MECs showed increased levels of IL-8 mRNA until 24 h post infection, whereas endothelial cells showed increased levels of IL-8 mRNA 24 h after infection, resulting in sustained IL-8 level in tissue [89]. Studies on bovine mammary endothelial cells demonstrated that in early reaction to E. coli infection vascular-derived PAF seems to play a prominent role [90].…”

Section: Function Of Endothelial Cells In Immune Response To Infectiomentioning

confidence: 99%

Stromal-Epithelial Interactions during Mammary Gland Development

Dzięgelewska¹,

Gajewska²

2019

Stromal Cells - Structure, Function, and Therapeutic Implications

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Mammary gland is an organ, which undergoes the majority of its development in the postnatal life of mammals. The complex structure of the mammary gland comprises epithelial and myoepithelial cells forming the parenchymal tissue and adipocytes, fibroblasts, vascular endothelial cells, and infiltrating immune cell composing the stromal compartment. During puberty and in adulthood, circulating hormones released from the pituitary and ovaries regulate the rate of development and functional differentiation of the mammary epithelium. In addition, growing body of evidence shows that interactions between the stromal and parenchymal compartments of the mammary gland play a crucial role in mammogenesis. This regulation takes place on a paracrine level, by locally synthesized growth factors, adipokines, and cytokines, as well as via direct cell-cell interactions. This chapter summarizes the current knowledge about the complex nature of interactions between the mammary epithelium and stroma during mammary gland development in different mammalian species.

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Interleukin-8 expression by mammary gland endothelial and epithelial cells following experimental mastitis infection with E. coli

Cited by 23 publications

References 36 publications

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

In vivo imaging of NF-κB activity during Escherichia coli-induced mammary gland infection

Stromal-Epithelial Interactions during Mammary Gland Development

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