Intermediate Filament Interactions in Neurons

Young, Kevin G.; Kothary, Rashmi

doi:10.1007/978-1-4419-6787-9_17

Cited by 2 publications

(2 citation statements)

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“…First, most developmental patterns only transiently reveal a specific set of zones; embryonic cluster markers are either suppressed or eventually mark all Purkinje cells (calbindin, engrailed1/2 , L7/Pcp2 , White and Sillitoe, 2012). Second, early postnatal “bridge” markers such neurogranin (Larouche et al, 2006) and phospholipase Cβ4 (PLCβ4; Marzban et al, 2007; Young and Kothary, 2011) do not reveal zones in the vermis and hemispheres throughout postnatal development, making it hard to examine how each stage of development is ultimately linked to the adult pattern of zones. Third, the onset of adult stripe patterns occurs only after postnatal day (P) 15 (Armstrong et al, 2001; Apps and Hawkes, 2009), which typically leaves a gap between when cluster and bridge makers delineate zones and when the well-understood framework of zebrin-like patterns are observed.…”

Section: Introductionmentioning

confidence: 99%

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Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression

White¹,

Sillitoe²

2013

Front. Neuroanat.

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The cerebellum is organized into parasagittal zones that control sensory-motor behavior. Although the architecture of adult zones is well understood, very little is known about how zones emerge during development. Understanding the process of zone formation is an essential step toward unraveling how circuits are constructed to support specific behaviors. Therefore, we focused this study on postnatal development to determine the spatial and temporal changes that establish zonal patterns during circuit formation. We used a combination of wholemount and tissue section immunohistochemistry in mice to show that the cytoskeletal protein neurofilament heavy chain (NFH) is a robust marker for postnatal cerebellar zonal patterning. The patterned expression of NFH is initiated shortly after birth, and compared to the domains of several known zonal markers such as zebrin II, HSP25, neurogranin, and phospholipase Cβ4 (PLCβ4), NFH does not exhibit transient expression patterns that are typically remodeled between stages, and the adult zones do not emerge after a period of uniform expression in all lobules. Instead, we found that throughout postnatal development NFH gradually reveals distinct zones in each cerebellar lobule. The boundaries of individual NFH zones sharpen over time, as zones are refined during the second and third weeks after birth. Double labeling with neurogranin and PLCβ4 further revealed that although the postnatal expression of NFH is spatially and temporally unique, its pattern of zones respects a fundamental and well-known molecular topography in the cerebellum. The dynamics of NFH expression support the hypothesis that adult circuits are derived from an embryonic map that is refined into zones during the first 3-weeks of life.

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Section: Introductionmentioning

confidence: 99%

“…The neurofilaments are a sub-family of proteins that comprise part of a larger family of intermediate cytoskeletal proteins (Young and Kothary, 2011). The three members are named according to their sizes: neurofilament light (NFL, 68 kDa), neurofilament medium (NFM, 160 kDa), and NFH (205 kDa) (Perrot et al, 2008).…”

Section: Introductionmentioning

confidence: 99%