Intermittent antibiotic treatment accelerated the development of colitis in IL-10 knockout mice

Li, Shiyu; Jin, Yusong; Fu, Wei; Cox, Abigail; Lee, Dale; Reddivari, Lavanya

doi:10.1016/j.biopha.2021.112486

Cited by 6 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To gain insights into the host response after vertically transmitted dysbiosis, we performed histology and a broad cytokine panel on murine colons and serum respectively (Supplementary Table S1). As seen in previous studies, we expected dysbiotic mice with induced colitis to display more severe clinical signs of inflammation compared with control mice with induced colitis, which highlights the importance of a diverse gut community on immune regulation (36)(37)(38). Surprisingly, we observed that offspring from dysbiotic dams and control mice displayed similar low grade colon inflammation with signs of neutrophil infiltration and slight surface damage at week 24, due to the chemically induced colitis from DSS (Figure 1A).…”

Section: Dysbiotic Mice Showed Minimal Differences From Control Mice ...supporting

confidence: 82%

Eubacterium rectaledetoxification mechanism increases resilience of the gut environment

Richie,

Wiechman,

Ingold

et al. 2024

Preprint

View full text Add to dashboard Cite

Lachnospiraceae members were highly detected in dysbiotic IL-10 KO mice that displayed similar physiological outcomes as control mice. Lachnospiraceae is a highly diverse family of microbes that have been shown to display both commensal and pathogenic characteristics in the colon environment. We investigated the impact of genetic variation in five Lachnospiraceae strains on lowering cellular inflammation and ROS levels. Cell free spent media (CFSM) fromEubacterium rectaleresulted in lowered ROS, and nitric oxide levels in stressed colon cells. We demonstrated through an array of multi-omics and molecular techniques that glutathione (GSH) biosynthesized byE. rectalewas able to alleviate host ROS damage. We further showed downregulation of cell stress and immune response genes by host RNA sequencing, which is evidence thatE. rectalemicrobial products promote recovery and alleviate ROS stress.

show abstract

Section: Dysbiotic Mice Showed Minimal Differences From Control Mice ...supporting

confidence: 82%

Eubacterium rectaledetoxification mechanism increases resilience of the gut environment

Richie,

Wiechman,

Ingold

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Mechanisms IL-10 knockout mice is an animal model used for the study of IBD; these animals spontaneously develop enterocolitis within 5–8 weeks of birth due to an uncontrolled immune response to resident intestinal flora [ 178 , 179 ]. People who have an IL-10 gene polymorphism also have an increased risk of developing colitis [ 180 ].…”

Section: Non-classical Vitamin D Actionsmentioning

confidence: 99%

The Role of Vitamin D in Health and Disease: A Narrative Review on the Mechanisms Linking Vitamin D with Disease and the Effects of Supplementation

Rebelos

Tentolouris

Jude

2023

Drugs

View full text Add to dashboard Cite

Vitamin D insufficiency or deficiency (VDD) is a very prevalent condition in the general population. Vitamin D is necessary for optimal bone mineralization, but apart from the bone effects, preclinical and observational studies have suggested that vitamin D may have pleiotropic actions, whereas VDD has been linked to several diseases and higher all-cause mortality. Thus, supplementing vitamin D has been considered a safe and inexpensive approach to generate better health outcomes—and especially so in frail populations. Whereas it is generally accepted that prescribing of vitamin D in VDD subjects has demonstrable health benefits, most randomized clinical trials, although with design constraints, assessing the effects of vitamin D supplementation on a variety of diseases have failed to demonstrate any positive effects of vitamin D supplementation. In this narrative review, we first describe mechanisms through which vitamin D may exert an important role in the pathophysiology of the discussed disorder, and then provide studies that have addressed the impact of VDD and of vitamin D supplementation on each disorder, focusing especially on randomized clinical trials and meta-analyses. Despite there already being vast literature on the pleiotropic actions of vitamin D, future research approaches that consider and circumvent the inherent difficulties in studying the effects of vitamin D supplementation on health outcomes are needed to assess the potential beneficial effects of vitamin D. The evaluation of the whole vitamin D endocrine system, rather than only of 25-hydroxyvitamin D levels before and after treatment, use of adequate and physiologic vitamin D dosing, grouping based on the achieved vitamin D levels rather than the amount of vitamin D supplementation subjects may receive, and sufficiently long follow-up are some of the aspects that need to be carefully considered in future studies.

show abstract

“…Administration of Lactobacillus species from birth prevented the development of enterocolitis in IL-10-deficient mice [ 121 ] and administration of an 8-strain probiotic to IL-10-deficient mice with established inflammation improved disease by normalising the epithelial barrier [ 122 ]. Although antibiotic treatment provides an attractive candidate for the treatment of colitis in patients with IL-10 deficiency, a recent study in IL-10-deficient animals demonstrated that intermittent antibiotic exposure accelerated the development of severe colitis in male mice [ 123 ].…”

Section: Spontaneous Colitismentioning

confidence: 99%

Inflammatory Bowel Disease: A Review of Pre-Clinical Murine Models of Human Disease

Katsandegwaza

Horsnell

Smith

2022

IJMS

View full text Add to dashboard Cite

Crohn’s disease (CD) and ulcerative colitis (UC) are both highly inflammatory diseases of the gastrointestinal tract, collectively known as inflammatory bowel disease (IBD). Although the cause of IBD is still unclear, several experimental IBD murine models have enabled researchers to make great inroads into understanding human IBD pathology. Here, we discuss the current pre-clinical experimental murine models for human IBD, including the chemical-induced trinitrobenzene sulfonic acid (TNBS) model, oxazolone and dextran sulphate sodium (DSS) models, the gene-deficient I-kappa-B kinase gamma (Iκκ-γ) and interleukin(IL)-10 models, and the CD4+ T-cell transfer model. We offer a comprehensive review of how these models have been used to dissect the etiopathogenesis of disease, alongside their limitations. Furthermore, the way in which this knowledge has led to the translation of experimental findings into novel clinical therapeutics is also discussed.

show abstract

Intermittent antibiotic treatment accelerated the development of colitis in IL-10 knockout mice

Cited by 6 publications

References 53 publications

Eubacterium rectaledetoxification mechanism increases resilience of the gut environment

Eubacterium rectaledetoxification mechanism increases resilience of the gut environment

The Role of Vitamin D in Health and Disease: A Narrative Review on the Mechanisms Linking Vitamin D with Disease and the Effects of Supplementation

Inflammatory Bowel Disease: A Review of Pre-Clinical Murine Models of Human Disease

Contact Info

Product

Resources

About