-A recently proposed self-consistent approach to the analysis of thermospheric and ionospheric long-term trends has been applied to Rome ionosonde summer noontime observations for the period. This approach includes: (i) a method to extract ionospheric parameter long-term variations; (ii) a method to retrieve from observed f o F 1 neutral composition (O, O 2 , N 2 ), exospheric temperature, Tex and the total solar EUV flux with l < 1050 Å; and (iii) a combined analysis of the ionospheric and thermospheric parameter long-term variations using the theory of ionospheric F-layer formation. Atomic oxygen, [O] period which is mainly due to atomic oxygen decrease after ∼1990. A linear trend in (dh m F 2 ) 11y estimated over the period is very small and insignificant reflecting the absence of any significant trend in neutral temperature. The retrieved neutral gas density, r atomic oxygen, [O] and exospheric temperature, Tex long-term variations are controlled by solar and geomagnetic activity, i.e. they have a natural origin. The residual trends estimated over the period of ∼5 solar cycles are very small (<0.5% per decade) and statistically.