Interspecies comparison of probiotics isolated from different animals

Abstract

Aim:The aim of the current study was to isolate and identify naturally occurring probiotic Lactobacillus species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in probiotics on the species level.Materials and Methods:A total of 44 fecal and milk samples were collected under aseptic conditions from cattle, buffalo, camel, sheep, goats, and fish. The samples were cultured, and the isolated strains were confirmed biochemically and molecularly using 16S rRNA multiplex polymerase chain reaction (PCR) analysis following DNA extraction from the bacterial isolates.Results:A total of 31 isolates identified as lactobacilli were isolated from cattle milk, goat feces, sheep feces, fish feces, buffalo milk, camel milk, and goats’ milk. Lactobacillus species were identified based on the size of the PCR product. The results showed that different species were different in their lactobacilli content. At the same time, there were some differences between individuals of the same species.Conclusion:The diversity of probiotic strains isolated from different animal species implies different types of benefits to the host. Although it would be both money - and time-consuming research, discovering the benefit of each of these strains may provide very important information for the health of both human and animal. Furthermore, transferring these beneficial effects either to individuals within the same species or between different species would be of great importance.

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

“…Most probiotic are bacteria, but there are also few non-bacteria microorganisms like yeast that belong to the probiotic family [7]. As an important member of lactic acid bacteria, Lactobacillus has become one of the most commonly used probiotic species among all probiotic species [8]. Health promotion by lactobacilli, which are generally regarded as safe (GRAS), makes them particularly interesting as a zootechnical additive [9,10].…”

Characterization and Functional Test of Canine Probiotics

“…Interestingly, the bacterial strains exhibited overall superior effects compared to LGG in the canine macrophage cell line (DH82) than in the murine macrophage cell line (RAW264.7). Host preference for probiotics have been studied for their beneficial effects on the host by effectively balancing the microbial environment of the gut and enhancing nutrient digestibility, growth, and immune status ( Ripamonti et al, 2011 ; Chiang et al, 2015 ; Cui et al, 2017 ; Dowarah et al, 2017 , 2018 ; Abdou et al, 2018 ). However, direct comparative studies based on host differences are still limited.…”

Recent Advances in Camel Milk Processing

“…Some of those strains were isolated in raw camel milk [ 79 ]. They belong to the genus Lactobacillus ( L. acidophilus, L. rhamnosus, L. gasseri, and L. delbrueckii ) [ 80 ]. Other strains belonging to the same genus ( L. plantarum, L. pentosus , and Lactococcus lactis ) were reported by Yateem et al [ 81 ].…”