Interspecific competition between Xanthopimpla stemmator Thunberg and Dentichasmias busseolae Heinrich (Hymenoptera: Ichneumonidae), pupal parasitoids attacking Chilo partellus (Lepidoptera: Crambidae) in East Africa

Muli, Benjamin K.; Schulthess, Fritz; Maranga, Rosebella; Kutima, Hellen; Jiang, Nanqing

doi:10.1016/j.biocontrol.2005.09.013

Cited by 11 publications

(4 citation statements)

References 25 publications

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“…1993; Heinz & Nelson 1996; Urbaneja et al . 2003; De Moraes & Mescher 2005; Batchelor, Hardy & Barrera 2006; Muli et al . 2006).…”

Section: Discussionunclassified

“…Detection of multiparasitism can provide information on competition between parasitoids that utilize the same host species. Interspecific competition for host resources can be detrimental to achieving biological control of a pest species (Levesque et al 1993;Heinz & Nelson 1996;Urbaneja et al 2003;De Moraes & Mescher 2005;Batchelor, Hardy & Barrera 2006;Muli et al 2006). In the current study, multiparasitism was low and there appeared to be little interspecific competition between the Peristenus species investigated.…”

Section: Discussionmentioning

confidence: 99%

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A large‐scale comparison of conventional and molecular methods for the evaluation of host–parasitoid associations in non‐target risk‐assessment studies

Gariépy

Kuhlmann

Gillott

et al. 2008

Journal of Applied Ecology

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Summary1. Accurate identification of natural enemies is the cornerstone of biological control, and methods that can separate closely related species are essential in ecological studies of parasitoids. Conventionally, host rearing and dissection are used to define the ecological host range of candidate biological control agents and assess host-specificity of parasitoids. However, molecular methods may be more suitable for the evaluation of host-parasitoid associations. 2. To demonstrate the utility of molecular diagnostics in ecological host-range studies on parasitoids of Lygus plant bugs, host rearing, dissection and multiplex polymerase chain reaction (PCR) analysis were used to estimate parasitism levels and parasitoid species composition (genus Peristenus ) in more than 26 000 field-collected target and non-target Miridae. 3. Parasitism levels estimated by conventional and molecular methods were similar but molecular analysis can detect parasitoids earlier than dissection and rearing. Parasitoid pupal mortality prevented the identification of more than 30% of individuals reared from non-target host material; however, paired samples analysed with the multiplex assay allowed the identity of these parasitoids to be inferred. Molecular methods can provide different, and generally more complete, parasitoid species composition information because the results are not confounded by the host and parasitoid mortality encountered in rearing. However, detection of a parasitoid in a host does not necessarily indicate survival to the adult stage. Further, molecular identification of parasitoid species may be restricted to those species for which PCR primers are available. 4. Synthesis and applications . For molecular diagnostic techniques to gain widespread adoption in ecological studies on natural enemy host range, they must provide information that is equivalent (or superior) to information obtained by conventional methods. Based on a large-scale case study, associations between Peristenus spp. and their mirid hosts were used to demonstrate the utility of molecular diagnostics in studies on parasitoid ecological host range; however, this approach can be extended to pre-release risk-assessment studies on other candidate biological control agents. Beyond agent identification, molecular diagnostics can facilitate and expedite pre-and post-release studies on the ecological host range of parasitoids, potential non-target effects, host-parasitoid associations and trophic interactions.

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“…1993; Heinz & Nelson 1996; Urbaneja et al . 2003; De Moraes & Mescher 2005; Batchelor, Hardy & Barrera 2006; Muli et al . 2006).…”

Section: Discussionunclassified

Section: Discussionmentioning

confidence: 99%

A large‐scale comparison of conventional and molecular methods for the evaluation of host–parasitoid associations in non‐target risk‐assessment studies

Gariépy

Kuhlmann

Gillott

et al. 2008

Journal of Applied Ecology

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“…2003; Batchelor et al. 2006; Muli et al. 2006), a better understanding of multiparasitism could provide insight into the level of interspecific competition in the field.…”

Section: Molecular Diagnostics In Parasitoid Researchmentioning

confidence: 99%

“…Accurate measurement of multiparasitism is important as it could provide information on parasitoid competition for a given host species. As interspecific competition for host resources can be detrimental to achieving biological control of a pest species (Heinz and Nelson 1996;Urbaneja et al 2003;Batchelor et al 2006;Muli et al 2006), a better understanding of multiparasitism could provide insight into the level of interspecific competition in the field.…”

Section: Braconidaementioning

confidence: 99%

Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

Gariépy

Kuhlmann

Gillott

et al. 2007

J Applied Entomology

174

144

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Abstract:The polymerase chain reaction (PCR) revolutionized the field of diagnostics, and today it has routine applications in medical, veterinary, forensic and botanical sciences. The fields of biological control and insect pest management have generally been slow to adopt PCR-based diagnostics in comparison with other fields of science. However, there has been increasing interest in the use of molecular diagnostic tools in arthropod biological control. In applied entomology, molecular techniques have generally been used for insect identification and systematics; however, PCR-based techniques are increasingly becoming recognized as valuable tools in ecological studies. Here, we review research that has used PCR-based techniques for parasitoid and predator/prey identification and detection, and place these studies in the context of their contributions to biological control of arthropods. The status and future directions of diagnostic molecular markers in applied entomology and insect pest management are also discussed.

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2014

The Braconid and Ichneumonid Parasitoid Wasps

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Interspecific competition between Xanthopimpla stemmator Thunberg and Dentichasmias busseolae Heinrich (Hymenoptera: Ichneumonidae), pupal parasitoids attacking Chilo partellus (Lepidoptera: Crambidae) in East Africa

Cited by 11 publications

References 25 publications

A large‐scale comparison of conventional and molecular methods for the evaluation of host–parasitoid associations in non‐target risk‐assessment studies

A large‐scale comparison of conventional and molecular methods for the evaluation of host–parasitoid associations in non‐target risk‐assessment studies

Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

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