Intestinal colonization of genotoxic Escherichia coli strains encoding colibactin and cytotoxic necrotizing factor in small mammal pets

Fabian, Niora; Mannion, Anthony; Feng, Yan; Madden, Carolyn M.; Fox, James G.

doi:10.1016/j.vetmic.2019.108506

Cited by 13 publications

(18 citation statements)

References 43 publications

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“…Cells treated with cyclomodulin-negative E. coli strains were indistinguishable from those treated with nonpathogenic K12 strain. These results were consistent with findings from previous studies showing cytotoxic effects of pks +, cnf + and cdt + E. coli in vitro 11 , 26 , 28 , 29 , 33 .…”

Section: Discussionsupporting

confidence: 93%

“…Syntenic alignments were confirmed for the pks island in all four predicted marmoset E. coli isolates with E. coli strains IHE3034 and NC101. (Supplementary Figure S5) Isolate S4 was found to have a putative hybrid gene for clbJ-K , similar to a gene sequence found previously in E. coli isolates from laboratory and pet rats 28 , 33 .…”

Section: Resultssupporting

confidence: 82%

“…Pathogenic E. coli strains typically belong to phylogroups B2 or D, whereas those in phylogroups A and B1 are usually considered commensals 37 . Ninety-eight percent of the cyclomodulin-encoding E. coli isolated from laboratory marmosets belong to phylogenetic group B2, which is consistent with strains isolated from humans and other species 11 , 26 – 28 , 33 , 38 . The distribution of phylogroups was significantly different in Colony B, which had 61% prevalence of the non-pathogenic phylogroup A, whereas the prevalence of phylogroup A in Colony A was only 3%, and it was not isolated in Colony C. Potentially pathogenic E. coli classified in phylogroup B2 had a 44% prevalence overall, and was associated with the presence of virulence factor genes pks , cnf and hlyA , especially in Colonies A and C. Marmoset colony B had a significantly lower prevalence of pks + (6%) and cnf + (0%) E. coli isolates than the other two colonies.…”

Section: Discussionsupporting

confidence: 74%

“…Isolate S4 exhibited cytotoxicity to HeLa cells similar to other pks + E. coli isolates tested (Supplementary Figure S4), suggesting the hybrid gene produces functional colibactin. Similar clbJ-K hybrid genes were found in E. coli isolates from laboratory and pet rats; and these isolates caused cytotoxicity and DNA damage in HeLa cells following in vitro infection 28 , 33 .…”

Section: Discussionmentioning

confidence: 60%

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Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

McCoy

Mannion

Feng

et al. 2021

Sci Rep

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Cyclomodulins are virulence factors that modulate cellular differentiation, apoptosis, and proliferation. These include colibactin (pks), cytotoxic necrotizing factor (cnf), and cytolethal distending toxin (cdt). Pathogenic pks+, cnf+, and cdt+ E. coli strains are associated with inflammatory bowel disease (IBD) and colorectal cancer in humans and animals. Captive marmosets are frequently afflicted with IBD-like disease, and its association with cyclomodulins is unknown. Cyclomodulin-encoding E. coli rectal isolates were characterized using PCR-based assays in healthy and clinically affected marmosets originating from three different captive sources. 139 E. coli isolates were cultured from 122 of 143 marmosets. The pks gene was detected in 56 isolates (40%), cnf in 47 isolates (34%), and cdt in 1 isolate (0.7%). The prevalences of pks+ and cnf+ E. coli isolates were significantly different between the three marmoset colonies. 98% of cyclomodulin-positive E. coli belonged to phylogenetic group B2. Representative isolates demonstrated cyclomodulin cytotoxicity, and serotyping and whole genome sequencing were consistent with pathogenic E. coli strains. However, the presence of pks+, cnf+, or cdt+ E. coli did not correlate with clinical gastrointestinal disease in marmosets. Cyclomodulin-encoding E. coli colonize laboratory common marmosets in a manner dependent on the source, potentially impacting reproducibility in marmoset models.

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Section: Discussionsupporting

confidence: 93%

Section: Resultssupporting

confidence: 82%

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 60%

See 2 more Smart Citations

Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

McCoy

Mannion

Feng

et al. 2021

Sci Rep

Self Cite

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“…Escherichia coli is a gram-negative, rod-shaped bacteria present in the guts of various animals, including mammals ( Jang et al, 2017 ). Most strains of E. coli are non-pathogenic but can cause opportunistic infections ( De Souza et al, 2019 ), and some highly pathogenic strains cause various intestinal diseases and sepsis in humans and animals ( Fabian et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Kim¹,

Kim²,

Kim³

et al. 2020

Food Sci Anim Resour

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Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae . One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4°C and 55°C, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.

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Characterization of genotoxin-encoding Escherichia coli isolated from specific-pathogen free cats with impaired fertility

Mannion

McGee

Feng

et al. 2022

Veterinary Microbiology

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Intestinal colonization of genotoxic Escherichia coli strains encoding colibactin and cytotoxic necrotizing factor in small mammal pets

Cited by 13 publications

References 43 publications

Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli

Characterization of genotoxin-encoding Escherichia coli isolated from specific-pathogen free cats with impaired fertility

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