Intestinal Health Benefits of the Water-Soluble Carbohydrate Concentrate of Wild Grape (Vitis thunbergii) in Hamsters

Huang, Ya-Ling; Chu, Hui-Fang; Dai, Fan-Jhen; Yu, Tzu-Yi; Chau, Chi‐Fai

doi:10.1021/jf300942p

Cited by 16 publications

(9 citation statements)

References 21 publications

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“…According to the methods described by Huang et al [ 28 ] with slight modifications, fecal samples were first homogenized with cold saline (0.9% w / v ) at a ratio of 1:10 ( w / v ). After centrifugation at 1006 g for 10 min, 2 mL of the supernatant fraction was acidified with 10 μL of isocaporic acid (internal standard) and 20 μL of 50% ( w / v ) sulfuric acid.…”

Section: Methodsmentioning

confidence: 99%

Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters

Kao

Chang

et al. 2018

Molecules

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This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats. Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp. in the large intestine. As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (−16%), total visceral fat (−17.4%), non-visceral fat levels (−20.3%), and total body fat (−19.2%). Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (−14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%). A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.

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Section: Methodsmentioning

confidence: 99%

Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters

Kao

Chang

et al. 2018

Molecules

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“…Short-chain fatty acids (SCFAs) analysis of feces. SCFAs were quantified according to the previously published description [28]. Briefly, fresh fecal samples were collected and homogenized with cold saline (NaCl 0.9%, w/v) at a ratio of 1:10 (w/v) prior to centrifugation for 10 min at 1,000 g. Collected supernatants were acidified with 20 μL of 50% (w/v) sulfuric acid containing isocaporic acid as an internal standard.…”

Section: Pro-inflammatory Cytokines Analysismentioning

confidence: 99%

Amelioration of 5-fluorouracil-induced intestinal mucositis by Streptococcus thermophilus ST4 in a mouse model

Shen

Chen

Chu³

et al. 2021

PLoS ONE

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Intestinal mucositis is a commonly encountered toxic side effect in patients undergoing 5-fluorouracil (5-FU)-based chemotherapy. Numerous studies have shown that probiotics enable improving chemotherapy-induced intestinal mucositis, but the beneficial effects of probiotics differ depending on the strain. Therefore, in the present studies we suggest that S. thermophilus ST4 separated from raw milk may assess mucoprotective activity in 5-FU-induced intestinal mucositis. In our causal-comparative study design, fifteen mice were randomized assigned into three groups (n = 5/each group): control group, 5-FU group and 5-FU+S. thermophilus ST4 group. The control group was orally administrated saline only, and the 5-FU group was followed by intraperitoneal injection of 5-FU for 3 days after 10-day saline administration, and the 5-FU+S. thermophilus ST4 group was intragastrically subjected for S. thermophilus ST4 once per day during the whole experiment, starting from the first day of the experiment, followed by 5-FU intraperitoneal injection for 3 days after 10-day S. thermophilus ST4 pretreatment. Diarrhea score, pro-inflammatory cytokines serum levels, intestinal histopathology and short chain fatty acid were assessed. Here, we demonstrated the beneficial effects of S. thermophilus ST4 derived from raw milk against 5-FU-induced intestinal mucositis, including body weight reduction, appetite loss and diarrhea. Intrinsically, S. thermophilus ST4 effectively maintained epithelium structure in small intestines and colons as well as reduced the intestinal inflammation. Besides, S. thermophilus ST4 significantly increased the expression of acetic acid, reinforcing the muco-protective effects. In conclusion, our results demonstrate that S. thermophilus ST4 supplementation ameliorates 5-FU-induced intestinal mucositis. This suggests probiotic may serve as an alternative therapeutic strategy for the prevention or management of 5-FU-induced mucositis in the future.

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“…According to the methods as described by Huang, Chu, Dai, Yu, and Chau (2012) with slight modifications, the SCFA concentrations in the fecal samples were determined. Fresh fecal sample was homogenized with cold saline (0.9% w/v) at a ratio of 1:10 (w/v), followed by centrifugation at 1,006g for 10 min.…”

Section: Determination Of Fecal Short-chain Fatty Acids (Scfas)mentioning

confidence: 99%

Changes in different fecal parameters with administration of bromelain and inulin in a rat model

Saw

Kao

Chen

et al. 2018

CyTA - Journal of Food

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The present study was to investigate the effects of administrating bromelain, inulin, or a mixture of these ingredients on different fecal parameters in a rat model. Our results showed that taking bromelain (120 CDU/kg body weight) apparently increased fecal moisture by 18% and declined fecal mucinase activity (−36.3%). The incorporation of inulin (260 mg/kg body weight) could also result in some desirable changes including increased fecal moisture by 19%, declined fecal mucinase activity (−43.9%). The feeding of same amounts of bromelain and inulin could lead to significant increases in the presumptive counts of Bifidobacterium and Lactobacilli as well as the concentrations of various fecal short chain fatty acids (by 54-95%). This study suggested that the consumption of bromelain and inulin together might exert favorable effects in improving certain fecal parameters, and provide more hints for the development of functional food formulations. Cambios en distintos parámetros fecales atribuibles a la administración de bromelina e inulina en un modelo en ratas RESUMEN El presente estudio se propuso investigar los efectos que la administración de bromelina, inulina o una mezcla de estos ingredientes tiene sobre distintos parámetros fecales en un modelo en ratas. Los resultados dan cuenta de que la administración de bromelina (120 CDU/kg de peso corporal) aparentemente aumentó la humedad fecal en 18% y disminuyó la actividad de la mucinasa fecal (−36.3%). Por otra parte, se constató que la administración de inulina (260 mg/kg de peso corporal) puede conllevar algunos cambios deseables, entre los que se incluye el incremento de humedad fecal en 19% y la reducción de la actividad de la mucinasa fecal (−43.9%). Alimentar a las ratas con cantidades iguales de bromelina e inulina puede producir aumentos significativos en los recuentos presuntivos de Bifidobacterium y Lactobacilli y en la concentración de varios ácidos grasos fecales de cadena corta (54-95%). Los resultados del estudio sugieren que el consumo de bromelina e inulina juntas puede provocar efectos favorables, mejorando ciertos parámetros fecales y proporcionando elementos para desarrollar ciertas formulaciones de alimentos funcionales.

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Intestinal Health Benefits of the Water-Soluble Carbohydrate Concentrate of Wild Grape (Vitis thunbergii) in Hamsters

Cited by 16 publications

References 21 publications

Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters

Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters

Amelioration of 5-fluorouracil-induced intestinal mucositis by Streptococcus thermophilus ST4 in a mouse model

Changes in different fecal parameters with administration of bromelain and inulin in a rat model

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