Intestinal Ischemia during Hypoxia and Experimental Sepsis as Observed by NADH Videofluorimetry and Quenching of PD-Porphine Phosphorescence

İnce, Can; Sluijs, J.P. van der; Sinaasappel, M.; Avontuur, J. A. M.; Coremans, J. M. C. C.; Bruining, H. A.

doi:10.1007/978-1-4615-1875-4_14

Cited by 14 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the assumption that tissue oxygenation can be preserved by maintaining its blood supply is derived from models that presume uniformly perfused capillaries. Conversely, septic shock is characterized by increased heterogeneity of microcirculatory blood flow, which implies the presence of zones of tissue receiving an adequate perfusion through capillaries with continuous flow in close proximity with zones with no microvascular perfusion due to capillaries with stopped flow (12), which leads to inhomogeneity of O 2 distribution and, therefore, to abnormal cellular respiration, such as has been observed using in vivo NADH videofluorimetry techniques (22). We observed a severe decline in the proportion of small vessels perfused at both the jejunal and sublingual mucosa at the time of shock with a subsequent increase in blood flow heterogeneity and decrease in functional capillary density.…”

Section: Discussionmentioning

confidence: 99%

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock

Ospina-Tascón

Marín

Echeverri

et al. 2017

Journal of Applied Physiology

View full text Add to dashboard Cite

Our observations suggest that dynamic changes in the heterogeneity of microvascular blood flow at the gut mucosa are closely related to mesenteric O2 extraction, thus supporting the role of decreasing functional capillary density and increased intercapillary distances on alterations of O2 uptake during development and resuscitation from septic shock. Addition of a low-fixed dose of dobutamine might reverse such flow heterogeneity, improving microcirculatory flow distribution and tissue O2 consumption.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock

Ospina-Tascón

Marín

Echeverri

et al. 2017

Journal of Applied Physiology

View full text Add to dashboard Cite

show abstract

“…This time-resolved technique is very suitable for use in vivo, because many of the problems encountered with intensity measurements, such as the absorption and scattering of light by tissue components, are eliminated. Although oxygen-dependent quenching of fluorescence has been used for the determination of [O 2 ] in vitro (1,2,6,8,9), such measurements have not been applied in vivo. The introduction of Pd-porphyrin phosphorescent dyes by Wilson et al (22,23) opened the way to in vivo measurements.…”

mentioning

confidence: 99%

“…The penetration depth of the excitation wavelength (532 nm) in biological tissues is only a few millimeters. On the surface of rat intestine, for example, we measured a difference in temperature of 6°C from the core temperature (6,14). If the quenching constant at 38°C is used to determine the [O 2 ] at which the temperature is in reality 6°C lower, a 17% error in the concentration is introduced for a t of 24 µs.…”

mentioning

confidence: 99%

Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo

Sinaasappel

İnce

1996

Journal of Applied Physiology

Self Cite

105

View full text Add to dashboard Cite

Quantitative measurement of oxygen concentrations in the microvasculature is of prime importance in issues related to oxygen transport to tissue. The introduction of the quenching of the Pd-porphyrin phosphorescence as oxygen sensor in vivo by Wilson et al. (J. Appl. Physiol. 74: 580-589, 1993) has provided in this context a major advance in this area of research. For in vivo application, the dye is coupled to albumin to restrict the dye to the circulation and to measure oxygen in the physiological range. In this study a phosphorimeter with a gated photomultiplier is presented and validated. Furthermore, a nonlinear-fit method using the Marquardt-Levenberg algorithm is used to calculate the decay time. With this new phosphorimeter, calibration measurements were performed to investigate the effects of pH, temperature, and diffusivity. The results present a preparation method for albumin coupling of the dye that eliminates the pH dependency of the quenching kinetics. Furthermore, the decreased oxygen diffusivity of serum was compared with that of water, and it was shown that calibration constants measured in water can be extrapolated to serum.

show abstract

“…Mapping tissue oxygenation in depth requires insertion of one or more electrodes into the organ, causing cell damage and bleeding, which severely limits the applicability of this technique. The introduction of phosphorimetry [29, 30], in contrast, has opened the field of non-invasive and online monitoring of microcirculatory oxygenation and has initiated numerous studies investigating the (patho)physiological mechanisms underlying several types of shock [8] and the effects of different treatment strategies [e.g., 11–13]. …”

Section: Discussionmentioning

confidence: 99%

Evaluation of multi-exponential curve fitting analysis of oxygen-quenched phosphorescence decay traces for recovering microvascular oxygen tension histograms

Bezemer

Faber

Almac

et al. 2010

Med Biol Eng Comput

View full text Add to dashboard Cite

Although it is generally accepted that oxygen-quenched phosphorescence decay traces can be analyzed using the exponential series method (ESM), its application until now has been limited to a few (patho)physiological studies, probably because the reliability of the recovered oxygen tension (pO2) histograms has never been extensively evaluated and lacks documentation. The aim of this study was, therefore, to evaluate the use of the ESM to adequately determine pO2 histograms from phosphorescence decay traces. For this purpose we simulated decay traces corresponding to uni- and bimodal pO2 distributions and recovered the pO2 histograms at different signal-to-noise ratios (SNRs). Ultimately, we recovered microvascular pO2 histograms measured in the rat kidney in a model of endotoxemic shock and fluid resuscitation and showed that the mean microvascular oxygen tension, 〈pO2〉, decreased after induction of endotoxemia and that after 2 h of fluid resuscitation, 〈pO2〉 remained low, but the hypoxic peak that had arisen during endotoxemia was reduced. This finding illustrates the importance of recovering pO2 histograms under (patho)physiological conditions. In conclusion, this study has characterized how noise affects the recovery of pO2 histograms using the ESM and documented the reliability of the ESM for recovering both low- and high-pO2 distributions for SNRs typically found in experiments. This study might therefore serve as a frame of reference for investigations focused on oxygen (re)distribution during health and disease and encourage researchers to (re-)analyze data obtained in (earlier) studies possibly revealing new insights into complex disease states and treatment strategies.

show abstract

Intestinal Ischemia during Hypoxia and Experimental Sepsis as Observed by NADH Videofluorimetry and Quenching of PD-Porphine Phosphorescence

Cited by 14 publications

References 8 publications

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock

Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo

Evaluation of multi-exponential curve fitting analysis of oxygen-quenched phosphorescence decay traces for recovering microvascular oxygen tension histograms

Contact Info

Product

Resources

About

Intestinal Ischemia during Hypoxia and Experimental Sepsis as Observed by NADH Videofluorimetry and Quenching of PD-Porphine Phosphorescence

Cited by 14 publications

References 8 publications

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O2 extraction during septic shock

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O2 extraction during septic shock

Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo

Evaluation of multi-exponential curve fitting analysis of oxygen-quenched phosphorescence decay traces for recovering microvascular oxygen tension histograms

Contact Info

Product

Resources

About

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock

Effects of dobutamine on intestinal microvascular blood flow heterogeneity and O₂ extraction during septic shock