Intestinal parameters of oxidative imbalance in celiac adults with extraintestinal manifestations

Abstract

AIMTo evaluate selected intestinal parameters of oxidative stress, and antioxidant capacity in adult celiac disease patients with extraintestinal manifestations.METHODSThe study involved 85 adult patients divided into the following subgroups: (1) patients with newly diagnosed celiac disease (CD) (n = 7); (2) celiac patients not adhering to a gluten-free diet (GFD) (n = 22); (3) patients with CD on the GFD (n = 31); and (4) patients with functional disorders of the gastrointestinal tract, serving as controls (n = 25). Celiac patients presented with non-classic symptoms or extraintestinal manifestations. Standard blood tests including serum antioxidant levels (uric acid, bilirubin, and vitamin D), celiac antibody levels, and histopathological status of duodenal biopsy specimens have been determined. The expression of mRNA for tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 10 (IL-10), superoxide dismutase (SOD), heat-shock protein 70 (HSP-70), hypoxia-inducible factor 1 (HIF-1α), and BAX in the duodenal mucosa of patients was analyzed by reverse transcriptase-polymerase chain reaction.RESULTSThe mean plasma uric acid level in patients with active CD (newly diagnosed and nonadherent patients) and treated celiac patients was significantly higher than in controls (260.17 ± 53.65 vs 190.8 ± 22.98, P < 0.001, and 261.7 ± 51.79 vs 190.8 ± 22.98, P < 0.001, respectively). The mean bilirubin concentration in active and treated celiac patients was significantly lower than in controls (8.23 ± 5.04 vs 10.48 ± 4.08, P < 0.05 and 8.06 ± 3.31 vs 10.48 ± 4.08, P < 0.05, respectively). The mean plasma vitamin D level was significantly lower in active celiac patients than in treated celiac patients and controls (19.37 ± 9.03 vs 25.15 ± 11.2, P < 0.05 and 19.37 ± 9.03 vs 29.67 ± 5.12, P < 0.001, respectively). The expression of TNF-α, IL-10, and HSP-70 mRNAs was significantly elevated in the celiac groups regardless of the diet when compared with controls. Patients on the GFD presented a significantly lower mRNA expression of TNF-α and IL-10 than in newly diagnosed and nonadherent patients (P < 0.05). The expression of SOD mRNA was significantly elevated in celiac patients compared with controls (P < 0.05), with a significant difference between treated and untreated patients (P < 0.05). The expression of HIF-1α mRNA and BAX mRNA was significantly higher in patients with active CD compared with controls and patients on GFD, while no difference was observed between the latter two groups.CONCLUSIONIncreased intestinal expression of HSP-70 despite GFD indicates that GFD only partially reduced oxidative stress. CD patients exhibited an oxidative imbalance and inflammatory response despite GFD. Uric acid may act as an important antioxidant in CD.

Comparison of DNA methylation profiles from saliva in Coeliac disease and non-coeliac disease individuals

“…Elevated uric acid levels are markers of oxidative stress and inflammation, where uric acid, an antioxidant is produced in response to inflammation and oxidative stress [32]. Individuals with CD have elevated uric acid serum levels when compared to healthy controls which may reflect the inflammation and oxidative stress that is characteristic of CD even when following a GFD [33]. Interestingly, the direction of methylation differences between the pilot and validation cohort were in opposite directions.…”

Existe Alteração em Marcadores Inflamatórios em Estudantes de Medicina após Participação em Programa Mente-Corpo?

“…De fato, esta citocina apresenta-se elevada em diversas condições de susceptibilidade ao estresse oxidativo, como nas do-enças autoimunes e doenças inflamatórias intestinais, e exerce ação importante para a redução dos danos inflamatórios 16,17,18 .…”

“…As medianas das idades do grupo intervenção e controle foram, respectivamente, 22 (18-32) e 22 (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28), sem diferença estatística (p = 0,910).…”

Investigating intestinal epithelium metabolic dysfunction in Celiac Disease using personalized genome-scale models

“…While counterintuitive, the increased activity of these three ROS-modulating enzymes in active CeD aligns with prior studies. For example, superoxide dismutase elevated activity has been documented in active CeD patients relative to remission CeD and healthy controls (39). It was proposed that gliadin may cause an imbalance in antioxidant activity, leading to the increased odds of lesions in the intestinal mucosa (40).…”