Intestinal permeability in Crohn's disease.

Murphy, M S; Eastham, E J; Nelson, Roger; Pearson, Adj; Laker, M. F.

doi:10.1136/adc.64.3.321

Cited by 56 publications

(37 citation statements)

References 19 publications

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“…Therefore, an abnormal LMR was due to higher excretion of lactulose in the urine than a reduced excretion of mannitol. Pearson et al [15] , Murphy et al [3] , Wyatt et al [7] , and Katz et al [27] have also reported similar findings. The probes used to measure IP are water soluble, which cannot penetrate the lipid cell membrane of enterocytes and thus use the paracellular route through the tight junctions (TJs) for per meation.…”

Section: Discussionsupporting

confidence: 66%

“…IP has been implicated in the pathogenesis and frequent relapses of Crohn's disease (CD) [2][3][4][5][6] . Seven to 18% higher relapse rate has been reported in patients with increased IP compared to those with normal IP [7][8][9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have reported a relationship between abnormal IP and the activity of the disease [3,5,37,38] . In some reports it has been observed that the magnitude of alterations in L/M ratio increases with the increase in disease activity index.…”

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confidence: 99%

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Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Benjamin¹,

Makharia²,

Ahuja³

et al. 2008

WJG

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INTRODUCTIONIntestinal permeability (IP) is the property of the intestinal epithelium which refers to the facility with which it allows molecules to pass through by non-mediated diffusion [1] . IP has been implicated in the pathogenesis and frequent relapses of Crohn's disease (CD) [2][3][4][5][6] . Seven to 18% higher relapse rate has been reported in patients with increased IP compared to those with normal IP [7][8][9][10][11] . Moreover, RAPID COMMUNICATIONIntestinal permeability and its association with the patient and disease characteristics in Crohn's disease Abstract AIM: To assess the intestinal permeability (IP) in patients with Crohn's disease (CD) and study the association of IP with the patient and disease characteristics.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

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Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Benjamin¹,

Makharia²,

Ahuja³

et al. 2008

WJG

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“…These either only low-Af r substances, or one containing radiohave been mannitol (1) and rhamnose (2), frequently in nuclides. A non-invasive test permitting the simultacomtynation with a disaccharide such s cellobiose (3, neous determination of human gut permeability to com-4) or lactulose (5)(6)(7). In many cases, radioactive sub-pounds in both the low and high relative molecular mass stances such s 51 Cr-EDTA (8)(9)(10), "Tc-DTPA (11), r nge not containing radioactive components would be desirable.…”

Section: Introductionmentioning

confidence: 99%

Parallel Determination of Gut Permeability in Man with M _r 400, M _r 1500, M _r 4000 and M _r 10 000 Polyethylene Glycol

Parlesak¹,

Bode

1994

CCLM

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Parlcsak and Bode: Gut pcrmeabiiity of K 400, M r 1500, M r 4000 and M r 10000 polyethylene glycol in man 813 Eur. J. Clin. Chcm. Clin. Biochem. Vol. 32, 1994, pp. 813-820 © 1994 Summary: Polyethylene glycol has been in use for a number of years for the assessment of gut permeability. The methods so far employed are usually limited to polyethylene glycols in the low relative molecular mass r nge (up to M T 1300). We developed a method for the simultaneous determination of gut permeability to M r 400, M r 1500, Af r 4000 and M r 10000 polyethylene glycol, by applying a single oral dose of an appropriate mixture of these polyethylene glycols. After extraction from 24 h-urine, M T 1500, A/ r 4000 and M T 10000 polyethylene glycol were quantified by size exclusion chromatography, while M r 400 polyethylene glycol was determined by reversed phase chromatography. The detection limit of polyethylene glycol in the relative molecular mass r nge between M r 1500 and Μ τ 10000 was found to be 0.2 mg/1 urine, and the detection limit of Af r 400 polyethylene glycol 5 mg/1 urine. Recovery of the polyethylene glycols (N = 6) were 86.6% (CV: 4.8%) for H 400, 94.1% (CV: 7.2%) for M r 1500, 97.1% (CV: 5.5%) for M r 4000 and 97.4% (CV: 5.6%) for M r 10000.

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“…Lactulose, on the other hand, is considered unable to be transported across healthy enterocytes (14). The urinary excretion ratio between higher and lower molecular weight sugars has been shown to be altered in different disease states, including villous atrophy (14), celiac disease (15), Crohn's disease, and acute gastroenteritis (7,14,16). Use of such sugar permeability tests for testing intestinal permeability to macromolecules or food proteins is questionable.…”

Section: Discussionmentioning

confidence: 99%

Increased β-Lactoglobulin Absorption during Rotavirus Enteritis in Infants: Relationship to Sugar Permeability

et al. 1991

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ABSTRACT. We studied absorption of the potentially allergenic protein P-lactoglobulin during acute rotavirus diarrhea in infants and assessed the relationship of this macromolecular absorption with intestinal sugar permeability. After oral rehydration, 38 patients with acute gastroenteritis were given orally a 100-ml solution containing 4 g (11.7 mmol/L) of lactulose and 0.8 g (4.4 mmol/L) of mannitol, and their recovery rate as shown in urine passed during the subsequent 5 h was measured. A blood sample was taken 2 h after a milk feed for ELISA measurement of P-lactoglobulin in circulating immune complexes. Twelve nondiarrhea patients were studied after an overnight fast as controls. Immune complexes containing P-lactoglobulin were found in the serum of all, but the levels [median (range)] were significantly higher in patients with rotavirus diarrhea [686 (36-4352 Thus, a significant correlation between P-lactoglobulin absorption and sugar permeability was found; Spearman's rank correlation coefficient = 0.42, p = 0.004. This correlation was not, however, direct but was due to an inverse relationship between urinary recovery of mannitol and serum P-lactoglobulin immune complexes. These results indicate that rotavirus gastroenteritis is associated with enhanced P-lactoglobulin absorption and elevated lactulose/mannitol permeability test results, but these represent different phenomena. (Pediatr Res 30: 290-293, 1991) Abbreviations CI, confidence intervals After birth, the maturation of the human gastrointestinal tract includes development of a mucosal bamer that selectively prevents a variety of potentially antigenic or toxic substances from entering the internal environment of the body. Although at birth highly impermeable to macromolecules, the gut has specialized transport mechanisms for these substances in the Peyer's patches and the villous epithelium (1). Thus even under physiologic conditions, antigenically active food proteins or their peptide fragments are absorbed and gain access to the immune systems of the body (2). In various pathologic conditions, such as animal models of viral gastroenteritis (3, 4), antigenic macromolecular absorption is increased, which raises the question of possible local or systemic sensitization. There is no direct evidence of such increased intestinal permeability to proteins in human infants. This reflects the difficulty of measuring intestinal permeability to macromolecules by noninvasive methods. Most often, gastrointestinal permeability is measured via probe molecules like mannitol and lactulose. Such dual sugar absorption tests have been recommended in clinical practice as the most appropriate means to measure intestinal integrity (5). However, there is no direct evidence to indicate that the sugar permeability tests also measure the absorption of macromolecules. Recent studies on animal models suggest that no such simple correlation exists (6). Therefore, the applications of the sugar permeability tests remain elusive.The purpose of the present study was to evaluat...

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Intestinal permeability in Crohn's disease.

Cited by 56 publications

References 19 publications

Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Parallel Determination of Gut Permeability in Man with M _r 400, M _r 1500, M _r 4000 and M _r 10 000 Polyethylene Glycol

Increased β-Lactoglobulin Absorption during Rotavirus Enteritis in Infants: Relationship to Sugar Permeability

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Intestinal permeability in Crohn's disease.

Cited by 56 publications

References 19 publications

Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Intestinal permeability and its association with the patient and disease characteristics in Crohn’s disease

Parallel Determination of Gut Permeability in Man with M r 400, M r 1500, M r 4000 and M r 10 000 Polyethylene Glycol

Increased β-Lactoglobulin Absorption during Rotavirus Enteritis in Infants: Relationship to Sugar Permeability

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Parallel Determination of Gut Permeability in Man with M _r 400, M _r 1500, M _r 4000 and M _r 10 000 Polyethylene Glycol