Intestinal toxicity and resistance gene threat assessment of multidrug-resistant Shigella: A novel biotype pollutant

Zhao, Jiahong; Wang, Qiujie; Xu, Yanbin; Li, Xinyan; Lin, Xinhua; Lin, Zitao; Luan, Tiangang

doi:10.1016/j.envpol.2022.120551

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…The carrying of resistance genes by poultry intestinal microorganisms has attracted considerable attention [52,53]. In the present study, we investigate the prevalence and diversity of ARGs in the gut microbiota of Jianghan chickens that did not receive antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

Metagenome-assembled genome reveals species and functional composition of Jianghan chicken gut microbiota and isolation of Pediococcus acidilactic with probiotic properties

Shen,

Wang,

Dong

et al. 2024

Microbiome

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Background Chickens are one of the most widely farmed animals worldwide and play a crucial role in meat and egg production. Gut microbiota is essential for chickens’ health, disease, growth, and egg production. However, native chickens such as Jianghan chickens have better meat and egg production quality than centralized chickens, their intestinal microbial diversity is richer, and the potential gut microbial resources may bring health benefits to the host. Results The bacterial species composition in the gut microbiota of Jianghan chickens is similar to that of other chicken breeds, with Phocaeicola and Bacteroides being the most abundant bacterial genera. The LEfSe analysis revealed significant differences in species composition and functional profiles between samples from Jingzhou and the other three groups. Functional annotation indicated that the gut microbiota of Jianghan chickens were dominated by metabolic genes, with the highest number of genes related to carbohydrate metabolism. Several antibiotic resistance genes (ARGs) were found, and the composition of ARGs was similar to that of factory-farmed chickens, suggesting that antibiotics were widely present in the gut microbiota of Jianghan chickens. The resistance genes of Jianghan chickens are mainly carried by microorganisms of the Bacteroidota and Bacillota phylum. In addition, more than 829 isolates were selected from the microbiota of Jianghan chickens. Following three rounds of acid and bile tolerance experiments performed on all the isolated strains, it was determined that six strains of Pediococcus acidilactici exhibited consistent tolerance. Further experiments confirmed that three of these strains (A4, B9, and C2) held substantial probiotic potential, with P. acidilactici B9 displaying the highest probiotic potential. Conclusions This study elucidates the composition of the intestinal microbiota and functional gene repertoire in Jianghan chickens. Despite the absence of antibiotic supplementation, the intestinal microbial community of Jianghan chickens still demonstrates a profile of antibiotic resistance genes similar to that of intensively reared chickens, suggesting resistance genes are prevalent in free-ranging poultry. Moreover, Jianghan and intensively reared chickens host major resistance genes differently, an aspect seldom explored between free-range and pastured chickens. Furthermore, among the 829 isolates, three strains of P. acidilatici exhibited strong probiotic potential. These findings provide insights into the unique gut microbiota of Jianghan chickens and highlight potential probiotic strains offering benefits to the host.

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Section: Discussionmentioning

confidence: 99%

Metagenome-assembled genome reveals species and functional composition of Jianghan chicken gut microbiota and isolation of Pediococcus acidilactic with probiotic properties

Shen,

Wang,

Dong

et al. 2024

Microbiome

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“…In Shigella spp., drug resistance is mainly because of0 their adeptness to survive and replicate in the human gastrointestinal tract while incorporating exogenous genetic material, including antimicrobial resistance (AMR) genes on mobile genetic elements, from other Gram-negative bacteria [3,10]. In recent years, multiple reports have demonstrated the isolation of multidrug-resistant (MDR) Shigella spp.…”

Section: Drivers Of Bacterial Resistancementioning

confidence: 99%

Resistance in Enteric Shigella and nontyphoidal Salmonella: emerging concepts

Yang,

Xiang,

Qiu

2023

Current Opinion in Infectious Diseases

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Purpose of review The emergence of globally resistant enteric Shigella and nontyphoidal Salmonella strains (NTS) has limited the selection of effective drugs, which has become a major challenge for the treatment of infections. The purpose of this review is to provide the current opinion on the antimicrobial-resistant enteric Shigella and nontyphoidal Salmonella. Recent findings Enteric Shigella and NTS are resistant to almost all classes of antimicrobials in recent years. Those with co-resistance to ciprofloxacin, azithromycin and ceftriaxone, the first-line antibiotics for the treatment of infectious diarrhoea have emerged worldwide. Some of them have caused interregional and international spread by travel, trade, MSM, and polluted water sources. Several strains have even developed resistance to colistin, the last-resort antibiotic used for treatment of multidrug-resistant Gram-negative bacteria infections. Summary The drug resistance of enteric Shigella and NTS is largely driven by the use of antibiotics and horizontal gene transfer of mobile genetic elements. These two species show various drug resistance patterns in different regions and serotypes. Hence treatment decisions for Shigella and Salmonella infections need to take into consideration prevalent antimicrobial drug resistance patterns. It is worth noting that the resistance genes such as bla CTX, mph, ermB, qnr and mcr, which can cause resistance to ciprofloxacin, cephalosporin, azithromycin and colistin are widespread because of transmission by IncFII, IncI1, IncI2 and IncB/O/K/Z plasmids. Therefore, continuous global monitoring of resistance in Shigella and Salmonella is imperative.

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“…1,2 Antibiotic treatments are classic and effective methods for bacterial infections while accelerating the evolution of multidrug resistance and serious environmental pollution. 3,4 Therefore, developing alternative antibacterial agents and strategies to escape bacterial resistance for the highly effective treatment of bacterial infections is highly desired. In recent years, nanozymes have attracted considerable attention as promising alternatives for combating bacteria due to their broad-spectrum antibacterial activities, negligible toxicity, and less drug resistance.…”

Section: Introductionmentioning

confidence: 99%

“…(4 mice per group) for different treatments: (I) control; (II) control + NIR; (III) H 2 O 2 ; (IV) H 2 O 2 + NIR; (V) PdMo bimetallene; (VI) PdMo bimetallene + NIR; (VII) PdMo bimetallene + H 2 O 2 ; and (VIII) PdMo bimetallene + H 2 O 2 + NIR. Taking the PdMo bimetallene + H 2 O 2 + NIR group as an example, 200 μL of PdMo bimetallene (final concentration: 100 μg mL −1 ), 200 μL of H 2 O 2 (final concentration: 100 μM),…”

mentioning

confidence: 99%

PdMo bimetallene nanozymes for photothermally enhanced antibacterial therapy and accelerated wound healing

Zhou,

Li,

Wang

et al. 2024

Dalton Trans.

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Intestinal toxicity and resistance gene threat assessment of multidrug-resistant Shigella: A novel biotype pollutant

Cited by 4 publications

References 48 publications

Metagenome-assembled genome reveals species and functional composition of Jianghan chicken gut microbiota and isolation of Pediococcus acidilactic with probiotic properties

Metagenome-assembled genome reveals species and functional composition of Jianghan chicken gut microbiota and isolation of Pediococcus acidilactic with probiotic properties

Resistance in Enteric Shigella and nontyphoidal Salmonella: emerging concepts

PdMo bimetallene nanozymes for photothermally enhanced antibacterial therapy and accelerated wound healing

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