Intra-articular injections of autologous platelet concentrates in dogs with surgical reparation of cranial cruciate ligament rupture

Silva, R. F.; Carmona, JU; Rezende, C.M.F.

doi:10.3415/vcot-12-06-0075

Cited by 37 publications

(25 citation statements)

References 26 publications

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“…It has been reported that OA in rabbits treated with mesenchymal cells derived from adipose tissue showed radiographic changes at 20 weeks post infiltration [60]. Contrary to a previous study [60], here, improvement in the signs of OA was not seen, however a progression of the signs was not seen either during the six month study, similar to those results observed in human [61] and veterinary studies where Silva et al reported no significant differences in the radiographic exam between animals treated with PRP and control group after cranial cruciate ligament surgery [62]. The radiological analysis used in this study was inconclusive.…”

Section: Resultssupporting

confidence: 74%

Hip Osteoarthritis in Dogs: A Randomized Study Using Mesenchymal Stem Cells from Adipose Tissue and Plasma Rich in Growth Factors

Cuervo

Rubio

Sopena

et al. 2014

IJMS

116

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Purpose: The aim of this study was to compare the efficacy and safety of a single intra-articular injection of adipose mesenchymal stem cells (aMSCs) versus plasma rich in growth factors (PRGF) as a treatment for reducing symptoms in dogs with hip osteoarthritis (OA). Methods: This was a randomized, multicenter, blinded, parallel group. Thirty-nine dogs with symptomatic hip OA were assigned to one of the two groups, to receive aMSCs or PRGF. The primary outcome measures were pain and function subscales, including radiologic assessment, functional limitation and joint mobility. The secondary outcome measures were owners’ satisfaction questionnaire, rescue analgesic requirement and overall safety. Data was collected at baseline, then, 1, 3 and 6 months post-treatment. Results: OA degree did not vary within groups. Functional limitation, range of motion (ROM), owner’s and veterinary investigator visual analogue scale (VAS), and patient’s quality of life improved from the first month up to six months. The aMSCs group obtained better results at 6 months. There were no adverse effects during the study. Our findings show that aMSCs and PRGF are safe and effective in the functional analysis at 1, 3 and 6 months; provide a significant improvement, reducing dog’s pain, and improving physical function. With respect to basal levels for every parameter in patients with hip OA, aMSCs showed better results at 6 months.

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Section: Resultssupporting

confidence: 74%

Hip Osteoarthritis in Dogs: A Randomized Study Using Mesenchymal Stem Cells from Adipose Tissue and Plasma Rich in Growth Factors

Cuervo

Rubio

Sopena

et al. 2014

IJMS

116

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“…[7][8][9] Orthobiologic products (such as mesenchymal stem cells, bone marrow aspirate concentrate, and platelets rich products) are showing promise as safe and effective treatments for musculoskeletal diseases including osteoarthritis in people and animals. [10][11][12][13][14][15][16][17][18] Platelets possess anabolic and anti-inflammatory properties attributed to the delivery of various growth factors and cell signaling molecules that can modulate inflammation and promote tissue healing. 19,20 Because various methods exist to create different platelet-derived orthobiologics, we define platelet-rich plasma (PRP) as any product in which the concentration of platelets is increased relative to whole blood and partially or fully isolated from leukocytes and red cells within a plasma medium.…”

Section: Introductionmentioning

confidence: 99%

“…15,[20][21][22] More recently, the use of platelet-rich products has been shown to help alleviate pain and/or improve function including objective gait analysis in dogs with both naturally occurring and experimental models of OA. [13][14][15][16]23 We hypothesized that a single intra-articular injection of pure PRP (no white or red cells within the product) into the more severely affected stifle would cause substantial positive changes in kinetic gait symmetry in dogs with unilateral or bilateral chronic osteoarthritis attributed to long-term (>12 months) naturally occurring cranial cruciate ligament disease. A validated pressuresensitive walkway system was employed at baseline and every 4 weeks for 12 weeks post-treatment to determine peak vertical force (PF) and vertical impulse (VI) normalized to body weight in all limbs and calculate selected modified symmetry indices based on prior literature from PVF.…”

Section: Introductionmentioning

confidence: 99%

<p>Assessment of a Single Intra-Articular Stifle Injection of Pure Platelet Rich Plasma on Symmetry Indices in Dogs with Unilateral or Bilateral Stifle Osteoarthritis from Long-Term Medically Managed Cranial Cruciate Ligament Disease</p>

Venator¹,

Frye²,

Gamble³

et al. 2020

VMRR

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These authors contributed equally to this workPurpose: The use of platelet-rich plasma (PRP) to treat canine osteoarthritis has gained support within the scientific community. PRP effects on pain control for degenerative joint disease induced by naturally occurring cranial cruciate ligament instability are limited, particularly in a cohort of dogs with chronic instability and osteoarthritis (>12 months), representing a commonly encountered clinical population that often defaults to medical management. The goal of this study was to assess the effects of a single intra-articular PRP injection into an effected stifle in this cohort, to assess response to treatment, quantitative kinetic data as it relates to percent body weight for peak vertical force (PVF) and vertical impulse (VI) were collected, and symmetry indices related to PVF were determined. Methods: Twelve dogs with unilateral or bilateral osteoarthritis with ruptured, nonstabilized cranial cruciate ligaments over 12 months duration were identified. Unilateral injections of 2.5 mL of a PRP preparation into the most severely affected stifle based on kinetic analysis was performed. Repeat pressure-sensitive walkway analysis was conducted monthly for 3 months. Peak vertical force (PVF) and vertical impulse (VI) were normalized to body weight and identified in all four limbs. Previously published symmetry indices regarding PVF were calculated, comparing the treated limb with the contralateral limb, ipsilateral forelimb, and contralateral forelimb. Results: After treatment, hind limb symmetry index (SI) regarding PVF showed improved symmetry, suggesting more weight placement at all-time points after injection of the most affected limb (p < 0.01). Further, PVF asymmetry indices assessing contralateral fore (CFL) and hind limb (CHL) as well as ipsilateral forelimb (IFL) revealing a significant decrease from baseline for CHL at week 4 (p = 0.02), but not weeks 8 and 12. The CFL showed decreased differences in symmetry from baseline at each time point (p = 0.03). There were no statistically significant changes in PVF or VI over time in treated dogs. Conclusion: A single injection of PRP improved kinetics for minimally 4 weeks with some data suggesting an effect for up to 12 weeks. Therefore, PRP might be a viable therapeutic option for instability and inflammation associated with chronic osteoarthritis due to cranial cruciate ligament disease in the non-surgical patient.

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“…PRP has been mostly used in horses [6, 8] and dogs [9, 10] for the treatment of tenodesmic lesions and joint diseases. Furthermore, there are studies about the use of several PRP-related hemoderivatives for the treatment of mastitis [11], reproductive problems [12], and sole ulcers [13] in cows with encouraging results.…”

Section: Introductionmentioning

confidence: 99%

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Gutiérrez

López

Giraldo

et al. 2017

Veterinary Medicine International

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There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.

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Intra-articular injections of autologous platelet concentrates in dogs with surgical reparation of cranial cruciate ligament rupture

Abstract: Our results indicate that autologous PC might improve functional outcome after intra-articular cranial cruciate ligament repair. The effect of PC when using other repair procedures warrants additional studies.

Cited by 37 publications

References 26 publications

Hip Osteoarthritis in Dogs: A Randomized Study Using Mesenchymal Stem Cells from Adipose Tissue and Plasma Rich in Growth Factors

Hip Osteoarthritis in Dogs: A Randomized Study Using Mesenchymal Stem Cells from Adipose Tissue and Plasma Rich in Growth Factors

<p>Assessment of a Single Intra-Articular Stifle Injection of Pure Platelet Rich Plasma on Symmetry Indices in Dogs with Unilateral or Bilateral Stifle Osteoarthritis from Long-Term Medically Managed Cranial Cruciate Ligament Disease</p>

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

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