Intra-Individual Coexistence of a Wolbachia Strain Required for Host Oogenesis With Two Strains Inducing Cytoplasmic Incompatibility in the Wasp Asobara Tabida

Dedeine, Franck; Vavre, Fabrice; Shoemaker, DeWayne; Boulétreau, Michel

doi:10.1111/j.0014-3820.2004.tb01595.x

Cited by 51 publications

(35 citation statements)

References 35 publications

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“…It is however only one of the three that is obligatory for successful oogenesis. The other two Wolbachia types are able to induce CI [119]. Not only is this system remarkably similar to that seen with Sxl in Drosophila [113], it is also similar to effects seen with Wolbachia infections in filarial nematodes [120].…”

Section: Other Wolbachia-induced Phenotypes?supporting

confidence: 66%

Wolbachia Symbiosis in Arthropods

Clark¹

2007

Issues in Infectious Diseases

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Wolbachia pipientis is an intracellular bacteria found within the cytoplasm of a high proportion of arthropods. Widespread in insects, Wolbachia is also commonly found in other arthropod groups, including mites, spiders and terrestrial isopods. Wolbachia are normally maternally inherited and have evolved a number of strategies to ensure transmission. These include: (1) feminization, the conversion of genetic males into females, (2) parthenogenesis, the production of diploid offspring without sexual reproduction, (3) male killing, the killing of infected males to the benefit of infected female siblings, and (4) cytoplasmic incompatibility, the inability of infected males to successfully fertilize eggs from either uninfected females or females infected with different Wolbachia types. In addition to this reproductive parasitism, Wolbachia can influence other aspects of host fitness, including host longevity, fecundity, fertility and host-parasitoid interactions. Wolbachia has been studied extensively in the context of host spermatogenesis, oogenesis and embryogenesis. These cytological studies of host-Wolbachia interactions are providing insights into the ways in which Wolbachia are able to manipulate hosts.

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Section: Other Wolbachia-induced Phenotypes?supporting

confidence: 66%

Wolbachia Symbiosis in Arthropods

Clark¹

2007

Issues in Infectious Diseases

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“…A. tabida is naturally infected by three strains of the intracellular bacterium Wolbachia ( w Atab1, w Atab2 and w Atab3): w Atab1 and w Atab2 induce cytoplasmic incompatibility, and only w Atab3 is required for oogenesis completion [6,25]. …”

Section: Methodsmentioning

confidence: 99%

“…In this study, we used the NA strain and a Pi-derived strain (Pi3). Pi3 was obtained by moderate antibiotic treatment, and contains only the obligatory Wolbachia strain w Atab3 [25]. The lines are stable, and have been maintained by regular sib-matings without antibiotic treatment for about 100 generations.…”

Section: Methodsmentioning

confidence: 99%

Influence of Wolbachia on host gene expression in an obligatory symbiosis

et al. 2012

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BackgroundWolbachia are intracellular bacteria known to be facultative reproductive parasites of numerous arthropod hosts. Apart from these reproductive manipulations, recent findings indicate that Wolbachia may also modify the host’s physiology, notably its immune function. In the parasitoid wasp, Asobara tabida, Wolbachia is necessary for oogenesis completion, and aposymbiotic females are unable to produce viable offspring. The absence of egg production is also associated with an increase in programmed cell death in the ovaries of aposymbiotic females, suggesting that a mechanism that ensures the maintenance of Wolbachia in the wasp could also be responsible for this dependence. In order to decipher the general mechanisms underlying host-Wolbachia interactions and the origin of the dependence, we developed transcriptomic approaches to compare gene expression in symbiotic and aposymbiotic individuals.ResultsAs no genetic data were available on A. tabida, we constructed several Expressed Sequence Tags (EST) libraries, and obtained 12,551 unigenes from this species. Gene expression was compared between symbiotic and aposymbiotic ovaries through in silico analysis and in vitro subtraction (SSH). As pleiotropic functions involved in immunity and development could play a major role in the establishment of dependence, the expression of genes involved in oogenesis, programmed cell death (PCD) and immunity (broad sense) was analyzed by quantitative RT-PCR. We showed that Wolbachia might interfere with these numerous biological processes, in particular some related to oxidative stress regulation. We also showed that Wolbachia may interact with immune gene expression to ensure its persistence within the host.ConclusionsThis study allowed us to constitute the first major dataset of the transcriptome of A. tabida, a species that is a model system for both host/Wolbachia and host/parasitoid interactions. More specifically, our results highlighted that symbiont infection may interfere with numerous pivotal processes at the individual level, suggesting that the impact of Wolbachia should also be investigated beyond reproductive manipulations.

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“…A. tabida is naturally infected by three strains of Wolbachia , among which one (\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$w$\end{document}Atab3) is necessary for oogenesis completion (32,33). However, when Wolbachia are removed by antibiotic treatment, the degree of oogenetic defect exhibits genetic variation within populations (34).…”

Section: Methodsmentioning

confidence: 99%

SNP calling from RNA-seq data without a reference genome: identification, quantification, differential analysis and impact on the protein sequence

Lopez-Maestre¹,

Brinza²,

Marchet³

et al. 2016

Nucleic Acids Res

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SNPs (Single Nucleotide Polymorphisms) are genetic markers whose precise identification is a prerequisite for association studies. Methods to identify them are currently well developed for model species, but rely on the availability of a (good) reference genome, and therefore cannot be applied to non-model species. They are also mostly tailored for whole genome (re-)sequencing experiments, whereas in many cases, transcriptome sequencing can be used as a cheaper alternative which already enables to identify SNPs located in transcribed regions. In this paper, we propose a method that identifies, quantifies and annotates SNPs without any reference genome, using RNA-seq data only. Individuals can be pooled prior to sequencing, if not enough material is available from one individual. Using pooled human RNA-seq data, we clarify the precision and recall of our method and discuss them with respect to other methods which use a reference genome or an assembled transcriptome. We then validate experimentally the predictions of our method using RNA-seq data from two non-model species. The method can be used for any species to annotate SNPs and predict their impact on the protein sequence. We further enable to test for the association of the identified SNPs with a phenotype of interest.

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Intra-Individual Coexistence of a Wolbachia Strain Required for Host Oogenesis With Two Strains Inducing Cytoplasmic Incompatibility in the Wasp Asobara Tabida

Cited by 51 publications

References 35 publications

Wolbachia Symbiosis in Arthropods

Wolbachia Symbiosis in Arthropods

Influence of Wolbachia on host gene expression in an obligatory symbiosis

SNP calling from RNA-seq data without a reference genome: identification, quantification, differential analysis and impact on the protein sequence

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