Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells

Abstract

Understanding the effects of magnetic iron nanoparticles (MINPs) on the immune response is vitally important for biomedical applications such as cancer therapy, disease diagnosis and novel cancer imaging. In this study, lipid modified MINPs were designed and prepared by introducing the neutral lipid DSPE-PEG or the zwitterionic lipid DSPE-PCB into hydrophobic MINPs through hydrophobic interaction (L-MINPs and ZL-MINPs, respectively). The effect of L-MINPs and ZL-MINPs on the intracellular accumulation and immune responses of three kinds of antigen processing cells was examined. The results indicated that the high cellular uptake efficiency of surface coated MINPs was strongly related to the nature of the coating lipid, with the zwitterionic lipid being more effective than PEGylated ones. Besides, the results from flow cytometry (FCM), confocal laser scanning microscopy (CLSM) and Prussian blue staining demonstrated a time- and concentration-dependent MINP internalization. The uptake of zwitterionic lipid modified MINPs (ZL-MINPs) induced very low cytotoxicity and a strong mixed Th1/Th2 type immune response. L-MINPs could induce a strong increase in pro-inflammatory cytokines with a slight secretion of Th2 cytokines. Besides, no IL-10 was observed in both groups, indicating that MINPs with lipid modification were absence of immunosuppression. In conclusion, this study addresses an important implication of the lipid type and Fe concentration on the immune stimulation of cells and supports the potential for further development of biomedical applications.

Adjuvant-free cellulose nanofiber vaccine induces permanent humoral immune response in mouse

“…According to previous literature, the delivery efficiency of antigens has a direct effect on the ability to induce the level of immune response [32,33]. So we speculate that it is a very effective strategy to improve the delivery efficiency of antigens through controlling the releasing process of antigen, so as to avoid the metabolism loss caused by the one-time high dose injection of antigens.…”

Towards bio-compatible magnetic nanoparticles: Immune-related effects, in-vitro internalization, and in-vivo bio-distribution of zwitterionic ferrite nanoparticles with unexpected renal clearance

“…NPs coated with 3-(dimethylamino)propylamine-substituted poly(maleic anhydride-alt-1-decene) did not induce a change in cytokine or chemokine levels [24] whereas NPs coated with distearoylphosphoethanolamine-polycarboxybetaine (DSPE-PCB) induced a strong mixed Th1/Th2 type immune response but did not change the IL-10 level. [25] In both cases, NPs were not tested for bacterial endotoxin.…”

Noninvasive Brain Tumor Imaging Using Red Emissive Carbonized Polymer Dots across the Blood–Brain Barrier

“…Subsequently, C6 cells were exposed to CPDs at different concentrations (50–200 μg mL –1 ) for 1 h. The cellular uptake of CPDs would also enhance when increasing the concentration of CPDs (Figure B). These results suggested that the internalization of CPDs into C6 cells involved in time- and dose-dependent procedures was consistent with the behaviors of other nanoparticles. − …”