Intracellular pH of Streptomyces pristinaespiralis is correlated to the sequential use of carbon sources during the pristinamycins-producing process

Corvini, Philippe F.-X.; Delaunay, Stéphane; Maujean, Frédéric; Rondags, Emmanuel; Vivier, H.; Goergen, Jean‐Louis; Germain, Pierre

doi:10.1016/j.enzmictec.2003.06.002

Cited by 20 publications

(20 citation statements)

References 42 publications

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“…It has a strong activity against methicillin-, penicillin-, and vancomycin-resistant bacteria and exhibits a prolonged postantibiotic effect [2,3]. Pristinamycin was produced by Streptomyces pristinaespiralis [4][5][6][7]. In the pristinamycin fermentation with S. pristinaespiralis, both mycelium growth and pristinamycin biosynthesis were inhibited by the pristinamycin itself [8].…”

Section: Introductionmentioning

confidence: 98%

Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis

Jin

2009

Appl Biochem Biotechnol

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Improving pristinamycin production from Streptomyces pristinaespiralis was performed by introducing the resistance gene ptr followed by selection for enhanced tolerance to pristinamycin and fermentation test. To transfer ptr into S. pristinaespiralis, an effective method was established for the first time by using the intergeneric conjugation of DNA from Escherichia coli to S. pristinaespiralis. The procedure was optimized with heat treatment, spore concentration, optimum medium used in conjugation, concentration of MgCl(2), etc. With the optimized conditions, the conjugation frequency was up to 1.36 x 10(-3) exconjugants per recipient. The procedure was used to transfer the ptr gene into S. pristinaespiralis, resulting in 146 exconjugants. These exconjugants were screened on the pristinamycin-resistant plates, and then the fermentation test subsequently. Finally, two strains (SPR1 and SPR2) were obtained with a high yield of 0.11 and 0.15 g/l, respectively, which is about six to eight times more than that of wild-strain ATCC25486. The subculture experiments indicated that the hereditary character of the high-producing S. pristinaespiralis SPR1 and SPR2 was stable. Our work suggests that introducing resistance gene ptr into S. pristinaespiralis could be the way to improve the production of pristinamycin through the enhancement of antibiotic tolerance.

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Section: Introductionmentioning

confidence: 98%

Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis

Jin

2009

Appl Biochem Biotechnol

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“…It has strong activity against methicillin-, penicillin-, and vancomycin-resistant bacteria, and exhibits a prolonged post-antibiotic effect (Qadri et al 1997;Abdel-Hamid and Phillips 2003). Pristinamycin is produced extracellularly by Streptomyces pristinaespiralis (Paquet et al 1992;Corvini et al 2000Corvini et al , 2004Voelker and Altaba 2001) but, during fermentation, it is enzymatically, degraded. Moreover, both the growth of S. pristinaespiralis and the synthesis of pristinamycin are inhibited by pristinamycin itself.…”

Section: Introductionmentioning

confidence: 99%

Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis

et al. 2006

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Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l(-1), by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l(-1), which was a 1.25-fold increase over fermentation without resin.

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“…However, there are very few reports aimed at the regulation and optimization for pristinamycins fermentation. It was reported that under conventional batch conditions, the final yield of pristinamycins was just about 100mg/L with the buffered synthetic medium even by a spontaneous mutant [14].…”

mentioning

confidence: 99%

“…In the recent decades, cloning and analysis of genes involved in pristinamycins synthesis has become the focal point [8][9][10]. Whereas, in the studies on fermentation process, research teams have mainly focused on the metabolic characteristics of carbon and nitrogen sources and the induction effect of some lactones [11][12][13][14]. However, there are very few reports aimed at the regulation and optimization for pristinamycins fermentation.…”

mentioning

confidence: 99%

Medium Optimization Based on Statistical Methodologies for Pristinamycins Production by Streptomyces pristinaespiralis

Jia

Jin

Mei

2007

Appl Biochem Biotechnol

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The optimization of nutrient levels for the production of pristinamycins by Streptomyces pristinaespiralis CGMCC 0957 in submerged fermentation was carried out using the statistical methodologies based on the Plackett-Burman design, the steepest ascent method, and the central composite design (CCD). First, the Plackett-Burman design was applied to evaluate the influence of related nutrients in the medium. Soluble starch and MgSO4 x 7H2O were then identified as the most significant nutrients with a confidence level of 99%. Subsequently, the concentrations of the two nutrients were further optimized using response surface methodology of CCD, together with the steepest ascent method. Accordingly, a second-order polynomial regression model was finally fitted to the experimental data. By solving the regression equation from the model and analyzing the response surface, the optimal levels for soluble starch and MgSO4 x 7H2O were determined as 20.95 and 5.67g/L, respectively. Under the optimized medium, the yield of pristinamycins in the shake flask and 5-L bioreactor could reach 1.30 and 1.01 g/L, respectively, which is the highest yield reported in literature to date.

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Intracellular pH of Streptomyces pristinaespiralis is correlated to the sequential use of carbon sources during the pristinamycins-producing process

Cited by 20 publications

References 42 publications

Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis

Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis

Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis

Medium Optimization Based on Statistical Methodologies for Pristinamycins Production by Streptomyces pristinaespiralis

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