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Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l(-1), by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l(-1), which was a 1.25-fold increase over fermentation without resin.

Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of the EBA. A new type of TiO 2 -densified cellulose beads as matrix for EBA is exploited through the method of water-in-oil suspension thermal regeneration. The typical physical properties of beads are wet density 1.21 g/cm 3 , specific surface area 38.7 m 2 /mL, porosity 83.7%, and water content 69.5%. The results indicate that the custom-assembled matrix can be derivated to function as an anion exchanger for EBA, by possessing a significant mechanical strength, stable hydrodynamics, and comparable protein breakthrough capacities with the commercial Streamline diethylaminoethyl (DEAE) adsorbent.

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