Intracellular ROS Scavenging Activity and Downregulation of Inflammatory Mediators in RAW264.7 Macrophage by Fresh Leaf Extracts of Pseuderanthemum palatiferum

Abstract

Beneficial antioxidant phytochemicals are found in many medicinal plants. Pseuderanthemum palatiferum (PP), a well-known Vietnamese traditional medicinal plant in Thailand, has long been used in folk medicine for curing inflammatory diseases, often with limited support of scientific research. Therefore, this study aimed to determine antioxidant and modulation of inflammatory mediators of ethanol and water extracts of PP (EEP and WEP, resp.). WEP had significantly higher phenolic and flavonoid levels and DPPH radical scavenging activity than EEP. However, EEP exhibited greater reducing power than WEP. A greater decrease of tert-butyl hydroperoxide-induced oxidative stress in RAW264.7 macrophage cells was also observed with EEP. Modulation of inflammatory mediators of EEP and WEP was evaluated on LPS plus IFN-γ-stimulated RAW264.7 cells. EEP more potently suppressed LPS plus IFN-γ-induced nitric oxide (NO) production than WEP. Both EEP and WEP also suppressed the expression of iNOS and COX-2 protein levels. Collectively, these results suggest that PP possesses strong antioxidant and anti-inflammatory properties.

<i>Pseuderanthemum palatiferum</i> (Nees) Radlk extract induces apoptosis via reactive oxygen species-mediated mitochondria-dependent pathway in A549 human lung cancer cells

“…Interestingly, certain properties of the cytotoxic effect observed in this study might be attributable to the presence of high phenolic compounds within the PPA extract as indicated in Table 1 and consistent with the results of previous studies [12,15]. Various bioactivities of phenolic compounds play an important role in anticarcinogenic properties and also contribute to the induction of apoptosis by inhibiting cancer cell growth, producing reactive oxygen and DNA damage in diverse cancer cells [18,19].…”

“…The phytochemical investigation of its leaves has revealed the presence of n-pentacosan-1-ol, βsitosterol, stigmasterol, β-sitosterol 3-O-βglucoside, stigmasterol 3-O-β-glucoside, kaempferol 3-methyl ether 7-O-β-glucoside, apigenin 7-O-β-glucoside [7,8]. Studies of its leaf extracts have reported their pharmacological effects to be anti-diabetic [9,10], antihypertensive [11], anti-lypolytic and α-amylase inhibitory [12], anti-microbial [13], antiinflammatory and antioxidant [14,15]. A recent study revealed that P. paltiferum exerted an anticancer effect on colon cancer [16].…”

Effect ofPseuderanthemum palatiferum(Nees) Radlk fresh leaf ethanolic extract on human breast cancer MDA-MB-231 regulated cell death

“…Both aqueous and ethanolic extracts from PP suppress inflammation. 11 Khonsung et al 38 reported that the water extract of PP reduces both mean arterial blood pressure and pulse rate of rodents. The extract possesses vasodilatation effects via suppression of nitric oxide synthase (NOS) expression, leading to decreased nitric oxide (NO), an oxidant/vasodilator.…”

Integration of Traditional and Western Medicine in Vietnamese Populations: A Review of Health Perceptions and Therapies

“…11 (9) 2016 1413 effect in hyperglycemic rats and prevented some complications of diabetes [27]. In addition, lignins, betulin, and lupeol, isolated from the plant's roots, show cytotoxic and antimicrobial activities [28]. In addition, specifically, RAW264.7 macrophages treated with P. palatiferum showed decreased oxidative stress and reduced iNOS and COX-2 protein levels, suggesting the plant possesses strong anti-oxidant and anti-inflammatory properties [27,29].…”