Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease

Haralambus, Rhea; Burgstaller, Joerg P.; Klukowska‐Rötzler, Jolanta; Steinborn, Ralf; Buchinger, Shelley; Gerber, Vinzenz; Brandt, Sabine

doi:10.1111/j.2042-3306.2010.00078.x

Cited by 40 publications

(30 citation statements)

References 24 publications

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“…Horses were only included in the study on histological confirmation of tumour type. The lesions of horses with ES were scored according to a previously established score for clinical disease that ranged from 3 to 19 . Inclusion criteria comprised a sarcoid disease severity score of 3 to 8 (mild ES manifestation) for ESM, a score of 14 to 19 (severe ES manifestation) for ESA, presence of a SCC originating from the conjunctiva for oSCC and presence of a SCC at the vulva or glans penis for gSCC.…”

Section: Methodsmentioning

confidence: 99%

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Bogedale

Jagannathan

Gerber

et al. 2019

Vet Comparative Oncology

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The aim of this study was to investigate microRNA (miRNA) differential expression in the two most common equine skin tumours, equine sarcoid (ES) and squamous cell carcinoma (SCC), and its potential influence on the tumour microenvironment at post‐transcriptional level. We investigated miRNA fingerprints in four subgroups: mild (ESM) and aggressive (ESA) ES and ocular SCC (oSCC) and genital SCC (gSCC). Three tumours and three control samples were included in each of the four subgroups. Following next generation sequencing, miRNA differential expression analysis using DESeq2 was carried out. Pathways associated with the human mature homologues of identified dysregulated miRNAs were predicted using DIANA‐ miRPath v3.0. When comparing tumour vs control tissue, 57 miRNAs in ESM, six in ESA, 47 in oSCC and zero in gSCC were found to be differentially expressed and may thus serve as potential diagnostic tissue biomarkers. Whereas, ES lesions in general were associated with downregulation of the miR‐200 family, which may trigger epithelial‐mesenchymal transition, ESM lesions were associated with upregulation of the proposed tumour‐suppressive miRNA cluster on equine chromosome 24. In contrast, the oSCC tumours showed downregulation of this cluster as well as downregulation of the miR‐34 family, which may favour oSCC tumour cell metabolism. To further validate the proposed diagnostic miRNA fingerprints and their suggested biological effects, further miRNA studies need to be carried out in larger study cohorts.

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Section: Methodsmentioning

confidence: 99%

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Bogedale

Jagannathan

Gerber

et al. 2019

Vet Comparative Oncology

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“…Much interest has been focused on the mechanisms through which BPV-1 and -2 transform equine fibroblasts, causing equine sarcoids. Interestingly, the number of viral DNA copies has been shown to be significantly higher in more aggressive sarcoids, compared with their slowgrowing counterparts [33]. Over the years, the expression of several cancer markers has been studied in equine sarcoids, such as the tumour suppressor gene fragile histidine triad (FHIT), which was found to be down-regulated [34] as in human cervical cancer [35] although, curiously, not in BPV-associated bovine urinary tumours [36].…”

Section: Bpv-induced Cell Transformationmentioning

confidence: 99%

Bovine papillomavirus: opening new trends for comparative pathology

Costa

Medeiros

2013

Arch Virol

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“…The levels of E5 m-RNA are higher in the nodular type of sarcoids as compared with the other clinical types, although a recent report indicates that the highest levels of E5 DNA are found in the lesions of horses suffering from an aggressive form of disease i.e. multiple, rapidly growing tumours of various type [72,73]. These data provide all evidence of active BPV infection in sarcoids supporting the role of E5 in cell transformation.…”

Section: Bpv E5 and E7 In Naturally Occurring Tumoursmentioning

confidence: 65%

Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?

Corteggio

Altamura

Roperto

et al. 2013

Infect Agents Cancer

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Bovine papillomaviruses (BPVs) are oncogenic DNA viruses, which mainly induce benign lesions of cutaneous and/or mucosal epithelia in cattle. Thirteen (BPV 1–13) different viral genotypes have been characterized so far. BPVs are usually species-specific but BPV 1/2 may also infect equids as well as buffaloes and bison and cause tumors in these species. BPV-induced benign lesions usually regress, however occasionally they develop into cancer particularly in the presence of environmental carcinogenic co-factors. The major transforming protein of BPV is E5, a very short hydrophobic, transmembrane protein with many oncogenic activities. E5 contributes to cell transformation through the activation of the cellular β receptor for the platelet-derived growth factor (PDGFβ-r), it also decreases cell surface expression of major histocompatibility complex class I (MHCI) causing viral escape from immunosurveillance, and plays a role in the inhibition of the intracellular communication by means of aberrant connexin expression. E7 is considered as a weak transforming gene, it synergies with E5 in cell transformation during cancer development. E7 expression correlates in vivo with the over-expression of β1-integrin, which plays a role in the regulation of keratinocytes proliferation and differentiation. Additionally, E7 is involved in cell-mediated immune responses leading to tumour rejection, in anoikis process by direct binding to p600, and in invasion process by upregulation of Matrix metalloproteinase1 (MMP-1) expression. Studies on the role of BPV E5 and E7 oncoproteins in naturally occurring tumours are of scientific value, as they may shed new light on the biological role of these two oncogenes in cell transformation.

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Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease

Abstract: The rapid determination of BPV viral load will give a reliable marker for disease severity and may also be considered when establishing a therapeutic strategy.

Cited by 40 publications

References 24 publications

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Differentially expressed microRNAs, including a large microRNA cluster on chromosome 24, are associated with equine sarcoid and squamous cell carcinoma

Bovine papillomavirus: opening new trends for comparative pathology

Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?

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