Intraluminal pressure triggers myogenic response via activation of calcium spark and calcium-activated chloride channel in rat renal afferent arteriole

Yip, Kay‐Pong; Balasubramanian, Lavanya; Chen, Kan; Wang, Lei; Liu, Ruisheng; Ribeiro‐Silva, Luisa; Sham, James S.K.

doi:10.1152/ajprenal.00239.2018

Cited by 13 publications

(13 citation statements)

References 57 publications

(74 reference statements)

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“…Isolated perfused afferent arteriole measurement of luminal diameters. Measurements of the afferent arteriole luminal diameter were performed on isolated perfused mouse afferent arterioles as previously described (7,11,31,34,35). In brief, the afferent arterioles with attached glomeruli were isolated and transferred to a temperature-regulated chamber mounted on an inverted microscope in media.…”

Section: Methodsmentioning

confidence: 99%

Activation of the cannabinoid receptor 2 increases renal perfusion

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Soni

Jiang

et al. 2019

Physiological Genomics

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Acute kidney injury (AKI) is an increasing clinical problem that is associated with chronic kidney disease progression. Cannabinoid receptor 2 (CB2) activation has been shown to mitigate some of the deleterious tubular effects due to AKI, but its role on the renal vasculature has not been fully described. In this study, we investigated the effects of our novel CB2 receptor agonist, SMM-295, on renal vasculature by assessing cortical perfusion with laser Doppler flowmetry and changes in luminal diameter with isolated afferent arterioles. In this study, intravenously infused SMM-295 (6 mg/kg) significantly increased cortical renal perfusion (13.8 ± 0.6%; P < 0.0001; n = 7) compared with vehicle (0.1 ± 1.5%; n = 10) normalized to baseline values in anesthetized C57BL/6J mice. This effect was not dependent upon activation of the CB1 receptor (met-anandamide; 6 mg/kg iv) and was predominantly abolished in Cnr2 knockout mice with SMM-295 (6 mg/kg iv). Ablation of the renal afferent nerves with capsaicin blocked the SMM-295-dependent increase in renal cortical perfusion, and the increased renal blood flow was not dependent upon products synthesized by cyclooxygenase or nitric oxide synthase. The increased renal perfusion by CB2 receptor activation is also attributed to a direct vascular effect, since SMM-295 (5 μM) engendered a significant 37 ± 7% increase ( P < 0.0001; n = 4) in luminal diameters of norepinephrine-preconstricted afferent arterioles. These data provide new insight into the potential benefit of SMM-295 by activating vascular and nonvascular CB2 receptors to promote renal vasodilation, and provide a new therapeutic target to treat renal injuries that impact renal blood flow dynamics.

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Section: Methodsmentioning

confidence: 99%

Activation of the cannabinoid receptor 2 increases renal perfusion

Pressly

Soni

Jiang

et al. 2019

Physiological Genomics

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“…When CaCC are activated, spontaneous transient inward currents (STICs) occur and cause depolarisation and vasoconstriction, thus playing a role in determining vasomotor tone. The balance between these two pathways varies from vessel to vessel and will determine the overall contractility of the VSM [cerebral artery ( Zhao et al, 2017 ) and renal arterioles ( Yip et al, 2018 )]. Ca 2+ sparks and associated CaCC-mediated STICs have been identified in both tonic and phasically contracting VSMs ( Wang et al, 1992 ; Large and Wang, 1996 ; Yip et al, 2018 ).…”

Section: Calcium-activated Chloride Currents In Vsmmentioning

confidence: 99%

“…The balance between these two pathways varies from vessel to vessel and will determine the overall contractility of the VSM [cerebral artery ( Zhao et al, 2017 ) and renal arterioles ( Yip et al, 2018 )]. Ca 2+ sparks and associated CaCC-mediated STICs have been identified in both tonic and phasically contracting VSMs ( Wang et al, 1992 ; Large and Wang, 1996 ; Yip et al, 2018 ).…”

Section: Calcium-activated Chloride Currents In Vsmmentioning

confidence: 99%

Calcium-Activated Chloride Channels in Myometrial and Vascular Smooth Muscle

2021

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In smooth muscle tissues, calcium-activated chloride channels (CaCC) provide the major anionic channel. Opening of these channels leads to chloride efflux and depolarization of the myocyte membrane. In this way, activation of the channels by a rise of intracellular [Ca2+], from a variety of sources, produces increased excitability and can initiate action potentials and contraction or increased tone. We now have a good mechanistic understanding of how the channels are activated and regulated, due to identification of TMEM16A (ANO1) as the molecular entity of the channel, but key questions remain. In reviewing these channels and comparing two distinct smooth muscles, myometrial and vascular, we expose the differences that occur in their activation mechanisms, properties, and control. We find that the myometrium only expresses “classical,” Ca2+-activated, and voltage sensitive channels, whereas both tonic and phasic blood vessels express classical, and non-classical, cGMP-regulated CaCC, which are voltage insensitive. This translates to more complex activation and regulation in vascular smooth muscles, irrespective of whether they are tonic or phasic. We therefore tentatively conclude that although these channels are expressed and functionally important in all smooth muscles, they are probably not part of the mechanisms governing phasic activity. Recent knockdown studies have produced unexpected functional results, e.g. no effects on labour and delivery, and tone increasing in some but decreasing in other vascular beds, strongly suggesting that there is still much to be explored concerning CaCC in smooth muscle.

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“…16,17 During in vitro experimentation, alterations in intraluminal pressure within arteries and arterioles are used to demonstrate myogenic behavior 18 following active (i.e., Ca 2+ is added to the PSS) and passive (Ca 2+ is not added to the PSS) 19 conditions. Although myogenic responsiveness has been demonstrated in arterioles 20,21 and small arteries, 20,22,23 including bone arteries, 24,25 to our knowledge the myogenic capabilities of bone marrow arterioles (i.e., the vessels encapsulated within the pressurized skeleton) are unknown.…”

mentioning

confidence: 94%

Myogenic autoregulation in bone marrow arterioles and in vivo intramedullary pressure in femora of conscious, female Long Evans rats

et al. 2021

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Blood supply to bone and marrow is critical for meeting the oxygen and nutrient requirements of the tissue, as well as for eliminating metabolic byproducts. In addition, blood supply to the skeleton ensures its fluidic nature, 1 allowing for IMP and IFF. Intramedullary pressure and IFF are critical for the maintenance of bone structure since alterations in these physiological parameters with mechanical loading elicit bone gain or loss. The generated shear stress on bone cell (e.g., osteoclasts, osteocytes, and osteoblasts) surfaces with fluctuations in IMP and IFF stimulates the production and subsequent release of a variety of factors (e.g., NO, PGE 2 , and ATP) 2-7 that

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Intraluminal pressure triggers myogenic response via activation of calcium spark and calcium-activated chloride channel in rat renal afferent arteriole

Cited by 13 publications

References 57 publications

Activation of the cannabinoid receptor 2 increases renal perfusion

Activation of the cannabinoid receptor 2 increases renal perfusion

Calcium-Activated Chloride Channels in Myometrial and Vascular Smooth Muscle

Myogenic autoregulation in bone marrow arterioles and in vivo intramedullary pressure in femora of conscious, female Long Evans rats

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