Intramammary Challenge with Escherichia coli Following Immunization with a Curli-Producing Escherichia coli

Todhunter, D.A.; Smith, Kenneth L.; Hogan, J.S.; Nelson, Lennart

doi:10.3168/jds.s0022-0302(91)78230-1

Cited by 14 publications

(11 citation statements)

References 10 publications

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“…A previous study investigated the response of Holstein and Jersey cows to Escherichia coli intramammary infection following immunization, however, because only three Jersey cows were included in that study, breed-dependent differences were not examined (Todhunter et al 1991). aureus-induced mastitis were evaluated in the current study.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of breed-dependent differences in the innate immune responses of Holstein and Jersey cows toStaphylococcus aureusintramammary infection

Bannerman

Springer

Paape

et al. 2008

Journal of Dairy Research

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Mastitis is one of the most prevalent diseases of cattle. Various studies have reported breed-dependent differences in the risk for developing this disease. Among two major breeds, Jersey cows have been identified as having a lower prevalence of mastitis than Holstein cows. It is well established that the nature of the initial innate immune response to infection influences the ability of the host to clear harmful bacterial pathogens. Whether differences in the innate immune response to intramammary infections explain, in part, the differential prevalence of mastitis in Holstein and Jersey cows remains unknown. The objective of the current study was to evaluate several parameters of the innate immune response of Holstein and Jersey cows to intramammary infection with Staphylococcus aureus, a common mastitis-inducing pathogen. To control for non-breed related factors that could influence these parameters, all cows were of the same parity, in similar stages of milk production, housed and managed under identical conditions, and experimentally infected and sampled in parallel. The following parameters of the innate immune response were evaluated: acute phase protein synthesis of serum amyloid A and lipopolysaccharide-binding protein; total and differential circulating white blood cell counts; milk somatic cell counts; mammary vascular permeability; milk N-acetyl-beta-d-glucosaminidase (NAGase) activity; and production of the cytokines, interferon (IFN)-gamma, interleukin (IL)-12, tumour growth factor(TGF)-alpha, and TGF-beta1. The temporal response of all of these parameters following infection was similar between Holstein and Jersey cows. Further, with the exception of changes in circulating neutrophils and NAGase activity, the overall magnitude of these parameters were also comparable. Together, these data demonstrate that the innate immune response of Holstein and Jersey cows to Staph. aureus intramammary infection remains highly conserved despite previously reported differences in mastitis prevalence, as well as genotypic and phenotypic traits, that exist between the two breeds.

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Section: Discussionmentioning

confidence: 99%

Evaluation of breed-dependent differences in the innate immune responses of Holstein and Jersey cows toStaphylococcus aureusintramammary infection

Bannerman

Springer

Paape

et al. 2008

Journal of Dairy Research

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“…The role of curli in the pathogenesis of bovine mastitis is unknown. When Todhunter et al (1991) examined the outcome of experimental IMI with a curli-positive strain in cows vaccinated with either a curli-positive or a curli-negative strain of E. coli, all 12 cows developed clinical mastitis, regardless of curli expression of the vaccine strain. Our study evaluated the immediate effects of curli as measured by its effect on milk yields in a limited time frame.…”

Section: Discussionmentioning

confidence: 99%

Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis

Dyer

Sriranganathan

Nickerson

et al. 2007

Journal of Dairy Science

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Curli are adhesive surface structures produced by some Escherichia coli and Salmonella strains that bind host proteins and activate inflammatory mediators. In this study, 61 E. coli isolates from 36 clinical cases of bovine mastitis were characterized using enterobacterial repetitive intergenic consensus-PCR and screened for their ability to produce curli. Effect of curli production on case recovery, based on a return to precase milk yield, was investigated for a subset of 43 isolates from 20 quarters of 19 cows. Thirty-five (57%) of 61 isolates were curli positive. Fifty-eight of the 61 isolates clustered into 2 clonal groups at 52% genetic similarity. Genetically diverse E. coli isolates were simultaneously cultured from individual cases. Twenty-three isolates from 13 cows were clustered in clonal group I, of which 5 cases (38%) were curli positive; 35 isolates from 22 cows were clustered in clonal group II, of which 15 cases (68%) were curli positive. No association was found between genetic similarity and phenotypic curli expression of isolates from cows with clinical E. coli mastitis cases. Phenotypic curli expression in isolates did not affect recovery of cows' milk yield to premastitis production levels.

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“…Quarter foremilk samples were collected 7, 5, and 3 d before bacterial challenge, immediately before challenge, 3, 6, 9, 12 15, 18, 21, and 24 h postchallenge, and 2, 3, 4, and 7 d after challenge. Sample collection and microbiological procedures were as previously described (Todhunter et al, 1991). All gram-negative isolates were identified by motility, lactose fermentation, citrate use, and triple-sugar-iron reaction (National Mastitis Council, 1999).…”

Section: Quarter Foremilk Samplesmentioning

confidence: 99%

Effects of Adjuvants on Safety and Efficacy of an Escherichia coli J5 Bacterin

Hogan

Cannon

Smith

et al. 2005

Journal of Dairy Science

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The effects of using a water-soluble adjuvant or an emulsified oil-based adjuvant on the safety, antibody titer, and clinical responses of an Escherichia coli J5 bacterin were tested in an experimental infection trial. Fifty-one cows were assigned to 17 blocks of 3. Two cows within each block of 3 were vaccinated with a commercially prepared E. coli J5 bacterin containing either a water-soluble adjuvant or the same bacterin preparation emulsified in oil. One cow in each block was an unvaccinated control. Cows were immunized at drying off and 42 d later. The right or left front mammary quarter of each experimental cow was challenged by intramammary infusion of E. coli 727 between 14 and 35 DIM. Areas of inflammation at the primary injection site were greater 1, 2, and 3 d following primary vaccination for bacterin containing oil-in-water adjuvant compared with bacterin containing water-soluble adjuvant. Whey anti-E. coli J5 IgG titers were higher at calving for cows vaccinated with bacterin containing oil-in-water adjuvant than for cows either vaccinated with bacterin containing water-soluble adjuvant or unvaccinated controls. Serum x-E. coli J5 IgG titers were higher at calving for vaccinated cows than for unvaccinated controls. Peak bacterial counts in milk from challenged quarters were greater for unvaccinated controls than for cows vaccinated with bacterin containing water-in-oil adjuvant. Bacterial counts in milk from challenged quarters and clinical score both were greater in unvaccinated controls than cows vaccinated with bacterin containing water-in-oil adjuvant between 12 and 24 h postchallenge. Clinical responses were similar between unvaccinated controls and cows vaccinated with bacterin containing water-soluble adjuvant.

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Intramammary Challenge with Escherichia coli Following Immunization with a Curli-Producing Escherichia coli

Cited by 14 publications

References 10 publications

Evaluation of breed-dependent differences in the innate immune responses of Holstein and Jersey cows toStaphylococcus aureusintramammary infection

Evaluation of breed-dependent differences in the innate immune responses of Holstein and Jersey cows toStaphylococcus aureusintramammary infection

Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis

Effects of Adjuvants on Safety and Efficacy of an Escherichia coli J5 Bacterin

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