Intravaginal Administration of Human Type III Collagen-Derived Biomaterial with High Cell-Adhesion Activity to Treat Vaginal Atrophy in Rats

You, Shuang; Liu, Shuaibin; Dong, Xin; Hu, Li; Zhu, Yun; Hu, Lina

doi:10.1021/acsbiomaterials.9b01649

Cited by 31 publications

(12 citation statements)

References 61 publications

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“…This may contribute to the repair of the vaginal epithelium. [ 34 ] In addition, the results of TNF‐ α analysis showed that after 3 days of treatment, TNF‐ α secretion increased remarkably in the control group, which may trigger a downstream inflammatory response (Figure 6b). After 7 days of treatment, the CSNG and the antibiotic groups showed a recovery in TNF‐ α expression levels.…”

Section: Resultsmentioning

confidence: 99%

Controlled Cascade‐Release and High Selective Sterilization by Core–Shell Nanogels for Microenvironment Regulation of Aerobic Vaginitis

Wang

Tang

et al. 2023

Adv Healthcare Materials

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Aerobic vaginitis (AV) is a gynecological disease associated with vaginal flora imbalance. The nonselective bactericidal nature of antibiotics and low customization rate of probiotic supplementation in existing treatments lead to AV recurrence. Here, a drug delivery strategy is proposed that works with the changing dynamics of the bacterial flora. In particular, a core-shell nanogel (CSNG) is designed to encapsulate prebiotic inulin and antimicrobial peptide Cath 30. The proposed strategy allows for the sequential release of both drugs using gelatinase produced by AV pathogenic bacteria, initially selectively killing pathogenic bacteria and subsequently promoting the proliferation of beneficial bacteria in the vagina. In a simulated infection environment in vitro, the outer layer of CSNGs, Cath 30 is rapidly degraded and potently killed the pathogenic bacterium Staphylococcus aureus at 2-6 h. CSNGs enhances proliferation of the beneficial bacterium Lactobacillus crispatus by more than 50% at 24 h. In a rat AV model, the drug delivery strategy precisely regulated the bacterial microenvironment while controlling the inflammatory response of the vaginal microenvironment. This new treatment approach, configured on demand and precisely controlled, offers a new strategy for the treatment of vaginal diseases.

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Section: Resultsmentioning

confidence: 99%

Controlled Cascade‐Release and High Selective Sterilization by Core–Shell Nanogels for Microenvironment Regulation of Aerobic Vaginitis

Wang

Tang

et al. 2023

Adv Healthcare Materials

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“…Col III is generally co-exists with Col I in connective tissue and the proportion of Col III is typically greater during the wound healing and collagen development [ 13 ]. Recently, we investigated the interactions between a recombinant humanized type III collagen (rhCol III) and human fibroblasts via in vitro study to find a similar cell adhesion results as reported in the literature [ 14 , 15 ]. To further investigate the biological effect of rhCol III in vivo and provide evidence of validity to the commercialization, an animal model with damaged skin was successfully made via ultraviolet radiation, and rhCol III was implanted on schedule in an observation period of 8 weeks in this study.…”

Section: Introductionmentioning

confidence: 82%

The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study

Wang

Qiu

et al. 2022

Bioactive Materials

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The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field, such as collagen or hyaluronic acid, etc. However, the biosafety and efficacy of these implants are still challenged. In this study, a skin damage animal model was prepared by UV-photoaging and recombinant humanized type III collagen (rhCol III) was applied as a bioactive material to implant in vivo to study its biological effect, comparing with saline and uncrosslinked hyaluronic acid (HA). Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment. Histological observation, specific gene expression and other molecular biological methods were applied by the end of the animal experiment. The results indicated that rhCol III could alleviate the skin photoaging caused by UV radiation, including reduce the thickening of epidermis and dermis, increase the secretion of Collagen I (Col I) and Collagen III (Col III) and remodel of extracellular matrix (ECM). Although the cell-material interaction and mechanism need more investigation, the effect of rhCol III on damaged skin was discussed from influence on cells, reconstruction of ECM, and stimulus of small biological molecules based on current results. In conclusion, our findings provided rigorous biosafety information of rhCol III and approved its potential in skin repair and regeneration. Although enormous efforts still need to be made to achieve successful translation from bench to clinic, the recombinant humanized collagen showed superiorities from both safety and efficacy aspects.

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“…Among natural polymers, such as chitosan, gelatin, silk and cellulose, collagen is a main composite in extracellular matrix and contributes as the most abundant protein in mammals [19a] . With its good biocompatibility, high cell affinity and low antigenicity, [19b] collagen has been widely used for biomedical applications, including guided bone regeneration, corneal reconstruction, [20] wound dressing, [21] therapeutic contact lenses, [22] etc. However, pure collagen membranes usually lack desirable mechanical properties, which may bring obstacles for clinical uses.…”

Section: Methodsmentioning

confidence: 99%

Dynameric Collagen Self‐Healing Membranes with High Mechanical Strength for Effective Cell Growth Applications

Zhang

Cui

et al. 2020

Chemistry A European J

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The fabrication of biocompatible adaptive materials with high stiffness and self‐healing properties for medical applications is a challenging endeavor. Collagen is a major extracellular matrix component acting as a substrate for cell adhesion and migration. Dynamers are constitutional polymers whose monomeric components are linked through reversible bonds, able to modify their constitution through reversible exchange of their components. In the current work, we demonstrate that the rational combination of collagen and dynameric networks connected with reversible covalent imine bonds is a very important and previously unreported strategy to provide biocompatible membranes with self‐healing ability and excellent mechanical strength. The key challenge in the construction of such membranes is the required adaptive interaction between collagen chains and the dynamic cross‐linkers, preventing the formation of defects. For example, by varying structure and molecular lengths of the dynamers, the tensile strength of the dynameric membranes reach over 80 MPa, more than 400 % higher than that observed for the reference collagen membrane, and the highest value for break strain found, was 19 %. The self‐healing properties were observed when reconnecting two membrane pieces or even from crushed status of the membranes. Moreover, both MTT assay and confocal laser scanning microscopy method demonstrated the good biocompatibility of the collagen membranes, leaving more than 90 % viability for NIH 3T3 cells after 24 h co‐culture.

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Intravaginal Administration of Human Type III Collagen-Derived Biomaterial with High Cell-Adhesion Activity to Treat Vaginal Atrophy in Rats

Cited by 31 publications

References 61 publications

Controlled Cascade‐Release and High Selective Sterilization by Core–Shell Nanogels for Microenvironment Regulation of Aerobic Vaginitis

Controlled Cascade‐Release and High Selective Sterilization by Core–Shell Nanogels for Microenvironment Regulation of Aerobic Vaginitis

The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study

Dynameric Collagen Self‐Healing Membranes with High Mechanical Strength for Effective Cell Growth Applications

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